3D bioprinting of photo‐crosslinkable silk methacrylate ( SilMA )‐polyethylene glycol diacrylate ( PEGDA ) bioink for cartilage tissue engineering

2021 ◽  
Author(s):  
Ashutosh Bandyopadhyay ◽  
Biman B. Mandal ◽  
Nandana Bhardwaj
Keyword(s):  
Tissue Engineering ◽  
Polyethylene Glycol ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue ◽  
3D Bioprinting ◽  
Polyethylene Glycol Diacrylate ◽  
Glycol Diacrylate

Polyethylene glycol diacrylate scaffold filled with cell-laden methacrylamide gelatin/alginate hydrogels used for cartilage repair

2021 ◽  
pp. 088532822110448
Author(s):  
Xiang Zhang ◽  
Zhenhao Yan ◽  
Guotao Guan ◽  
Zijing Lu ◽  
Shujie Yan ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Polyethylene Glycol ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue ◽  
High Concentration ◽  
Polyethylene Glycol Diacrylate ◽  
Low Concentration ◽  
Glycol Diacrylate

Natural cartilage tissue has excellent mechanical properties and has certain cellular components. At this stage, it is a great challenge to produce cartilage scaffolds with excellent mechanical properties, biocompatibility, and biodegradability. Hydrogels are commonly used in tissue engineering because of their excellent biocompatibility; however, the mechanical properties of commonly used hydrogels are difficult to meet the requirements of making cartilage scaffolds. The mechanical properties of high concentration polyethylene glycol diacrylate (PEGDA) hydrogel are similar to those of natural cartilage, but its biocompatibility is poor. Low concentration hydrogel has better biocompatibility, but its mechanical properties are poor. In this study, two different hydrogels were combined to produce cartilage scaffolds with good mechanical properties and strong biocompatibility. First, the PEGDA grid scaffold was printed with light curing 3D printing technology, and then the low concentration GelMA/Alginate hydrogel with chondral cells was filled into the PEGDA grid scaffold. After a series of cell experiments, the filling hydrogel with the best biocompatibility was screened out, and finally the filled hydrogel with cells and excellent biocompatibility was obtained. Cartilage tissue engineering scaffolds with certain mechanical properties were found to have a tendency of cartilage formation in in vitro culture. Compared with the scaffold obtained by using a single hydrogel, this molding method can produce a tissue engineering scaffold with excellent mechanical properties on the premise of ensuring biocompatibility, which has a certain potential application value in the field of cartilage tissue engineering.

scholarly journals Evaluation of Polyethylene Glycol Diacrylate-Polycaprolactone Scaffolds for Tissue Engineering Applications

2017 ◽  
Vol 8 (3) ◽  
pp. 39 ◽  
Author(s):  
Hari Kotturi ◽  
Alaeddin Abuabed ◽  
Haris Zafar ◽  
Elaine Sawyer ◽  
Bipin Pallipparambil ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Polyethylene Glycol ◽  
Engineering Applications ◽  
Polyethylene Glycol Diacrylate ◽  
Glycol Diacrylate

scholarly journals Biomimetic hydrogels designed for cartilage tissue engineering

2021 ◽  
Author(s):  
Kresanti D. Ngadimin ◽  
Alexander Stokes ◽  
Piergiorgio Gentile ◽  
Ana M. Ferreira
Keyword(s):  
Tissue Engineering ◽  
Hyaluronic Acid ◽  
Polyethylene Glycol ◽  
Chondroitin Sulfate ◽  
Cartilage Tissue Engineering ◽  
Cartilage Regeneration ◽  
Cartilage Tissue

Cartilage-like hydrogels based on materials like gelatin, chondroitin sulfate, hyaluronic acid and polyethylene glycol are reviewed and contrasted, revealing existing limitations and challenges on biomimetic hydrogels for cartilage regeneration.

scholarly journals Translational Application of 3D Bioprinting for Cartilage Tissue Engineering

2021 ◽  
Vol 8 (10) ◽  
pp. 144
Author(s):  
Sophie McGivern ◽  
Halima Boutouil ◽  
Ghayadah Al-Kharusi ◽  
Suzanne Little ◽  
Nicholas J. Dunne ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cartilage Repair ◽  
Treatment Options ◽  
Cartilage Damage ◽  
Cartilage Tissue Engineering ◽  
Cartilage Regeneration ◽  
Bioactive Molecules ◽  
Cartilage Tissue ◽  
Layer By Layer ◽  
3D Bioprinting

Cartilage is an avascular tissue with extremely limited self-regeneration capabilities. At present, there are no existing treatments that effectively stop the deterioration of cartilage or reverse its effects; current treatments merely relieve its symptoms and surgical intervention is required when the condition aggravates. Thus, cartilage damage remains an ongoing challenge in orthopaedics with an urgent need for improved treatment options. In recent years, major advances have been made in the development of three-dimensional (3D) bioprinted constructs for cartilage repair applications. 3D bioprinting is an evolutionary additive manufacturing technique that enables the precisely controlled deposition of a combination of biomaterials, cells, and bioactive molecules, collectively known as bioink, layer-by-layer to produce constructs that simulate the structure and function of native cartilage tissue. This review provides an insight into the current developments in 3D bioprinting for cartilage tissue engineering. The bioink and construct properties required for successful application in cartilage repair applications are highlighted. Furthermore, the potential for translation of 3D bioprinted constructs to the clinic is discussed. Overall, 3D bioprinting demonstrates great potential as a novel technique for the fabrication of tissue engineered constructs for cartilage regeneration, with distinct advantages over conventional techniques.

scholarly journals 3D Bioprinted Implants for Cartilage Repair in Intervertebral Discs and Knee Menisci

2021 ◽  
Vol 9 ◽  
Author(s):  
Kalindu Perera ◽  
Ryan Ivone ◽  
Evelina Natekin ◽  
Cheryl. A. Wilga ◽  
Jie Shen ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cartilage Repair ◽  
Donor Site ◽  
Cartilage Tissue Engineering ◽  
The United States ◽  
Intervertebral Discs ◽  
The Body ◽  
Cartilage Tissue ◽  
Cartilage Defects ◽  
3D Bioprinting

Cartilage defects pose a significant clinical challenge as they can lead to joint pain, swelling and stiffness, which reduces mobility and function thereby significantly affecting the quality of life of patients. More than 250,000 cartilage repair surgeries are performed in the United States every year. The current gold standard is the treatment of focal cartilage defects and bone damage with nonflexible metal or plastic prosthetics. However, these prosthetics are often made from hard and stiff materials that limits mobility and flexibility, and results in leaching of metal particles into the body, degeneration of adjacent soft bone tissues and possible failure of the implant with time. As a result, the patients may require revision surgeries to replace the worn implants or adjacent vertebrae. More recently, autograft – and allograft-based repair strategies have been studied, however these too are limited by donor site morbidity and the limited availability of tissues for surgery. There has been increasing interest in the past two decades in the area of cartilage tissue engineering where methods like 3D bioprinting may be implemented to generate functional constructs using a combination of cells, growth factors (GF) and biocompatible materials. 3D bioprinting allows for the modulation of mechanical properties of the developed constructs to maintain the required flexibility following implantation while also providing the stiffness needed to support body weight. In this review, we will provide a comprehensive overview of current advances in 3D bioprinting for cartilage tissue engineering for knee menisci and intervertebral disc repair. We will also discuss promising medical-grade materials and techniques that can be used for printing, and the future outlook of this emerging field.

scholarly journals Characterization and Application of Carboxymethyl Chitosan-Based Bioink in Cartilage Tissue Engineering

2020 ◽  
Vol 2020 ◽  
pp. 1-11 ◽  
Author(s):  
Yunfan He ◽  
Soroosh Derakhshanfar ◽  
Wen Zhong ◽  
Bingyun Li ◽  
Feng Lu ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Mechanical Performance ◽  
Ethylenediaminetetraacetic Acid ◽  
Cell Attachment ◽  
Cartilage Tissue Engineering ◽  
Mechanical Analysis ◽  
Cartilage Tissue ◽  
3D Bioprinting ◽  
Modified Chitosan

Chitosan is a promising natural biomaterial for biological application; however, the weak mechanical performance of pristine chitosan limits its further utilization in hard tissue (such as cartilage) engineering. In this study, a chitosan-based 3D printing bioink with suitable mechanical properties was developed as 3D bioprinting ink for chondrocyte support. Chitosan was first modified by ethylenediaminetetraacetic acid (EDTA) to provide more carboxyl groups followed by physical crosslinking with calcium to increase the hydrogel strength. Dynamic mechanical analysis was carried out to evaluate viscoelastic properties with the addition of modified chitosan. A bioink with a combination of modified and pristine chitosan was formulated for scaffold fabrication via 3D bioprinting technique. Furthermore, cell viability, cell proliferation, and expression of chondrogenic markers were evaluated in vitro in chondrocytes loaded on the bioink. The novel bioink exhibited a favorable mechanical property and promoted cell attachment and chondrogenic gene expression in chondrocytes. Based on these results, we can conclude that the presented bioink could qualify for use in 3D bioprinting in cartilage tissue engineering.

Sign in / Sign up

Export Citation Format

Share Document