Characterization of the neuropilin-1 promoter; gene expression is mediated by the transcription factor Sp1

2002 ◽  
Vol 88 (4) ◽  
pp. 744-757 ◽  
Author(s):  
Mireille Rossignol ◽  
Jacques Pouysségur ◽  
Michael Klagsbrun
Keyword(s):  
Gene Expression ◽  
Transcription Factor ◽  
Transcription Factor Sp1 ◽  
Neuropilin 1 ◽  
Promoter Gene

The Drosophila dCREB-A gene is required for dorsal/ventral patterning of the larval cuticle

Development ◽  
1997 ◽  
Vol 124 (1) ◽  
pp. 181-193 ◽  
Author(s):  
D.J. Andrew ◽  
A. Baig ◽  
P. Bhanot ◽  
S.M. Smolik ◽  
K.D. Henderson
Keyword(s):  
Gene Expression ◽  
Transcription Factor ◽  
Signaling Cascades ◽  
Loss Of Function ◽  
Larval Cuticle ◽  
Extracellular Signals ◽  
Patterning Genes ◽  
Lateral Structures ◽  
Cuticular Structures

We report on the characterization of the first loss-of-function mutation in a Drosophila CREB gene, dCREB-A. In the epidermis, dCREB-A is required for patterning cuticular structures on both dorsal and ventral surfaces since dCREB-A mutant larvae have only lateral structures around the entire circumference of each segment. Based on results from epistasis tests with known dorsal/ventral patterning genes, we propose that dCREB-A encodes a transcription factor that functions near the end of both the DPP- and SPI-signaling cascades to translate the corresponding extracellular signals into changes in gene expression. The lateralizing phenotype of dCREB-A mutants reveals a much broader function for CREB proteins than previously thought.

scholarly journals Overexpression of Transcription Factor Sp1 Leads to Gene Expression Perturbations and Cell Cycle Inhibition

PLoS ONE ◽  
2009 ◽  
Vol 4 (9) ◽  
pp. e7035 ◽  
Author(s):  
Emmanuelle Deniaud ◽  
Joël Baguet ◽  
Roxane Chalard ◽  
Bariza Blanquier ◽  
Lilia Brinza ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Cycle ◽  
Transcription Factor ◽  
Transcription Factor Sp1 ◽  
Cell Cycle Inhibition

Isolation and functional characterization of Spirulina D6D gene promoter: Role of a putative GntR transcription factor in transcriptional regulation of D6D gene expression

2008 ◽  
Vol 365 (4) ◽  
pp. 643-649 ◽  
Author(s):  
Sanjukta Subudhi ◽  
Pavinee Kurdrid ◽  
Apiradee Hongsthong ◽  
Matura Sirijuntarut ◽  
Supapon Cheevadhanarak ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transcription Factor ◽  
Transcriptional Regulation ◽  
Functional Characterization ◽  
Gene Promoter

scholarly journals The Applications of Promoter-gene-Engineered Biosensors

Sensors ◽  
2018 ◽  
Vol 18 (9) ◽  
pp. 2823 ◽  
Author(s):  
Yingzhu Feng ◽  
Zhangzhang Xie ◽  
Xuanlong Jiang ◽  
Zhen Li ◽  
Yuping Shen ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transcription Factor ◽  
High Throughput Screening ◽  
Large Scale ◽  
Value Added ◽  
Site Directed Mutagenesis ◽  
Biomedical Science ◽  
Genetic Circuit ◽  
Genomic Libraries ◽  
Promoter Gene

A promoter is a small region of a DNA sequence that responds to various transcription factors, which initiates a particular gene expression. The promoter-engineered biosensor can activate or repress gene expression through a transcription factor recognizing specific molecules, such as polyamine, sugars, lactams, amino acids, organic acids, or a redox molecule; however, there are few reported applications of promoter-enhanced biosensors. This review paper highlights the strategies of construction of promoter gene-engineered biosensors with human and bacteria genetic promoter arrays with regard to high-throughput screening (HTS) molecular drugs, the study of the membrane protein’s localization and nucleocytoplasmic shuttling mechanism of regulating factors, enzyme activity, detection of the toxicity of intermediate chemicals, and probing bacteria density to improve value-added product titer. These biosensors’ sensitivity and specificity can be further improved by the proposed approaches of Mn2+ and Mg2+ added random error-prone PCR that is a technique used to generate randomized genomic libraries and site-directed mutagenesis approach, which is applied for the construction of bacteria’s “mutant library”. This is expected to establish a flexible HTS platform (biosensor array) to large-scale screen transcription factor-acting drugs, reduce the toxicity of intermediate compounds, and construct a gene-dynamic regulatory system in “push and pull” mode, in order to effectively regulate the valuable medicinal product production. These proposed novel promoter-engineered biosensors aiding in synthetic genetic circuit construction will maximize the efficiency of the bio-synthesis of medicinal compounds, which will greatly promote the development of microbial metabolic engineering and biomedical science.

Characterization of a spliced variant of human IRF-3 promoter and its regulation by the transcription factor Sp1

2012 ◽  
Vol 39 (6) ◽  
pp. 6987-6993 ◽  
Author(s):  
Wei Ren ◽  
Liang-Hua Zhu ◽  
Hua-Guo Xu ◽  
Rui Jin ◽  
Guo-Ping Zhou
Keyword(s):  
Transcription Factor ◽  
Transcription Factor Sp1 ◽  
Spliced Variant

scholarly journals Transcriptional Regulation of BACE1, the β-Amyloid Precursor Protein β-Secretase, by Sp1

2004 ◽  
Vol 24 (2) ◽  
pp. 865-874 ◽  
Author(s):  
Michelle A. Christensen ◽  
Weihui Zhou ◽  
Hong Qing ◽  
Anna Lehman ◽  
Sjaak Philipsen ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transcription Factor ◽  
Amyloid Precursor Protein ◽  
Proteolytic Processing ◽  
Precursor Protein ◽  
Transcriptional Level ◽  
Response Element ◽  
App Processing ◽  
Transcription Factor Sp1 ◽  
Β Amyloid

ABSTRACT Proteolytic processing of the β-amyloid precursor protein (APP) at the β site is essential to generate Aβ. BACE1, the major β-secretase involved in cleaving APP, has been identified as a type 1 membrane-associated aspartyl protease. We have cloned a 2.1-kb fragment upstream of the human BACE1 gene and identified key regions necessary for promoter activity. BACE1 gene expression is controlled by a TATA-less promoter. The region of bp −619 to +46 is the minimal promoter to control the transcription of the BACE1 gene. Several putative cis-acting elements, such as a GC box, HSF-1, a PU box, AP1, AP2, and lymphokine response element, are found in the 5′ flanking region of the BACE1 gene. Transcriptional activation and gel shift assays demonstrated that the BACE1 promoter contains a functional Sp1 response element, and overexpression of the transcription factor Sp1 potentiates BACE gene expression and APP processing to generate Aβ. Furthermore, Sp1 knockout reduced BACE1 expression. These results suggest that BACE1 gene expression is tightly regulated at the transcriptional level and that the transcription factor Sp1 plays an important role in regulation of BACE1 to process APP generating Aβ in Alzheimer's disease.

scholarly journals Negative Regulation of DsbA-L Gene Expression by the Transcription Factor Sp1

Diabetes ◽  
2014 ◽  
Vol 63 (12) ◽  
pp. 4165-4171 ◽  
Author(s):  
Q. Fang ◽  
W. Yang ◽  
H. Li ◽  
W. Hu ◽  
L. Chen ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transcription Factor ◽  
Negative Regulation ◽  
Transcription Factor Sp1
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