scholarly journals Establishment of a new protein C detection system based on chromogenic substrate assay and its clinical diagnostic value for deep vein thrombosis

2021 ◽  
Author(s):  
Wenfei Lu ◽  
Anshan Ge ◽  
Jinxia Zhao ◽  
Hui Rong ◽  
Chao Zhu ◽  
...  
Keyword(s):  
Deep Vein Thrombosis ◽  
Protein C ◽  
Detection System ◽  
Diagnostic Value ◽  
Chromogenic Substrate ◽  
Vein Thrombosis ◽  
Clinical Diagnostic ◽  
Deep Vein ◽  
New Protein

D-Dimers, Thrombin Antithrombin III Complexes and Prothrombin Fragments 1 + 2: Diagnostic value in Clinically Suspected Deep Vein Thrombosis

1991 ◽  
Vol 65 (01) ◽  
pp. 028-032 ◽  
Author(s):  
B Boneu ◽  
G Bes ◽  
H Pelzer ◽  
P Sié ◽  
H Boccalon
Keyword(s):  
Deep Vein Thrombosis ◽  
Antithrombin Iii ◽  
High Sensitivity ◽  
Diagnostic Value ◽  
Predictive Values ◽  
Vein Thrombosis ◽  
High Negative Predictive Value ◽  
Deep Vein ◽  
D Dimer ◽  
Mercury Strain Gauge

SummaryThis study was performed to determine the accuracy of D-Dimer fibrin derivatives, thrombin-antithrombin III (TAT) complexes and prothrombin fragments 1 + 2 (F 1 + 2) determinations for the diagnosis of deep vein thrombosis (DVT). One hundred and sixteen consecutive patients referred to the angiology unit of our hospital for a clinically suspected DVT were investigated. They were submitted to mercury strain gauge plethysmography and to ultrasonic duplex scanning examination; in cases of inconclusive results or of proximal DVT (n = 35), an ascending phlebography was performed. After these investigations were completed, the diagnosis of DVT was confirmed in 34 and excluded in 82. One half of the patients were already under anticoagulant therapy at the time of investigation. The 3 biological markers were assayed using commercially available ELISA techniques and the D-Dimer was also assayed with a fast latex method. The normal distribution of these markers was established in 40 healthy blood donors. The most accurate assay for the diagnosis of DVT was the D-Dimer ELISA which had both a high sensitivity (94%) and a high negative predictive value (95%). The D-Dirner latex, TAT complexes and F 1 + 2 were far less sensitive and provided negative predictive values which ranged between 78 and 85%. In spite of positive and significant correlations between the levels of ihe 3 markers, their association did not improve their overall accuracy for detecting D\/L Therefore, with the exception of the D-Dimer ELISA, these markers were of little value for the diagnosis of DVT in this specific population.

Diagnostic Value of Plasma Thrombin-Antithrombin III Complex and D-Dimer Concentration in Patients with Varicose Veins for Exclusion of Deep-Vein Thrombosis

1998 ◽  
Vol 91 (2) ◽  
pp. 101-104 ◽  
Author(s):  
Tomio Kawasaki ◽  
Nobutoshi Shinoki ◽  
Shin-ichi Iwamoto ◽  
Hironobu Fujimura ◽  
Norihide Yoshikawa ◽  
...  
Keyword(s):  
Deep Vein Thrombosis ◽  
Antithrombin Iii ◽  
Varicose Veins ◽  
Diagnostic Value ◽  
Vein Thrombosis ◽  
Deep Vein ◽  
Thrombin Antithrombin Iii Complex ◽  
D Dimer

Homozygous deficiency of protein c with neonatal deep vein thrombosis and new symptoms at young age

1993 ◽  
Vol 70 ◽  
pp. S107
Author(s):  
V. De Stefano ◽  
S. Mastrangelo ◽  
M.S. Iovino ◽  
P. Pola ◽  
R. Flore ◽  
...  
Keyword(s):  
Deep Vein Thrombosis ◽  
Protein C ◽  
Young Age ◽  
Vein Thrombosis ◽  
Deep Vein

scholarly journals Frequency of Thrombophilic Gene Mutations in Patients with Deep Vein Thrombosis and in Women with Recurrent Pregnancy Loss

2017 ◽  
Vol 12 (1) ◽  
pp. 162-166 ◽  
Author(s):  
Mahmoud Mohamed Elgari ◽  
Nadir Ahmed Ibrahim ◽  
Abdel Rahim Mahmoud Muddathir ◽  
Faris Mergheni Eltoom ◽  
Ibrahim M Ibrahim
Keyword(s):  
Deep Vein Thrombosis ◽  
Protein C ◽  
Pregnancy Loss ◽  
Recurrent Pregnancy Loss ◽  
Factor V Leiden ◽  
Protein S ◽  
Prothrombin G20210a ◽  
Factor V ◽  
Vein Thrombosis ◽  
Deep Vein

AbstractThrombophilia may be anticipated by single or combined hereditary defects in encoding genes factor V, Prothrombin, and MTHFR. The aim of this study was to determine the prevalence and associated risks of V Leiden (G1691A), Prothrombin (G20210A), and MTHFR (C677T) mutations in Saudi women with Deep Vein Thrombosis (DVT) and women with recurrent pregnancy loss (RPL). Protein C and protein S activity were measured to determine combined effects, if any. We examined 60 women with a history of DVT and 60 with RPL, extracted DNA from EDTA blood and determined three mutations by using multiplex PCR reactions followed by Strip Assay KIT. Pro C Global assay was used to determine the cutoff value [PCATNR = 0.80]. Protein C/S chromogenic assay was used to estimate protein C and S percentages. Frequency of Factor V Leiden G/A genotype in patients with DVT 7 (11.6%) had a significant association for DVT χ2 (OR = 5.1, P = 0.03). In women with RPL the three mutations did not show any significant association, levels of Protein C, protein S and PCAT-NR in patient groups not different from controls (P > 0.05). In conclusion, we recommend expanding on these data to provide larger-scale studies.

scholarly journals Diagnostic Value of Neutrophil Lymphocyte Ratio and D-Dimer as Biological Markers of Deep Vein Thrombosis in Patients Presenting with Unilateral Limb Edema

2021 ◽  
Vol Volume 12 ◽  
pp. 313-325
Author(s):  
Ikhwan Rinaldi ◽  
Rachmat Hamonangan ◽  
Mohamad Syahrir Azizi ◽  
Rahmat Cahyanur ◽  
Fadila Wirawan ◽  
...  
Keyword(s):  
Deep Vein Thrombosis ◽  
Biological Markers ◽  
Diagnostic Value ◽  
Neutrophil Lymphocyte Ratio ◽  
Lymphocyte Ratio ◽  
Vein Thrombosis ◽  
Limb Edema ◽  
Deep Vein ◽  
D Dimer

Protein C Inhibitor and Other Components of the Protein C Pathway in Patients with Acute Deep Vein Thrombosis during Heparin Treatment

1990 ◽  
Vol 63 (03) ◽  
pp. 380-382 ◽  
Author(s):  
D Tabernero ◽  
F España ◽  
V Vicente ◽  
A Estellés ◽  
J Gilabert ◽  
...  
Keyword(s):  
Deep Vein Thrombosis ◽  
Protein C ◽  
Normal Values ◽  
Protein S ◽  
Mean Value ◽  
Vein Thrombosis ◽  
Heparin Treatment ◽  
Protein C Inhibitor ◽  
Acute Deep Vein Thrombosis ◽  
Deep Vein

SummaryThe protein C inhibitor (PCI) concentration and other parameters of the protein C pathway were studied in 19 patients with symptomatic acute deep vein thrombosis before and during the first 5 days of heparin treatment. The mean levels of PCI antigen and activity decreased rapidly and significantly during the first day of heparin therapy from 83 and 81% to 60 and 59% of the pooled normal human plasma (p <0.01), respectively, and to 56 and 54% after 5 days of treatment (p <0.01). In contrast, antithrom-bin III decreased progressively 25% during 5 days of heparin treatment. Protein C antigen and activity and total protein S remained unchanged during heparin treatment. Free protein S was decreased before heparin treatment (83%, p <0.05) and increased to normal values after 5 days of treatment. C4b-binding protein was significantly increased before and during heparin treatment (p <0.01). Activated protein C (APC) complexed to its two major plasma inhibitors, PCI and α1-antitrypsin (α1AT) were measured by specific ELISA’s. Before treatment, 18 of the 19 patients studied had increased levels of APC: α1AT complexes with a mean value of 27 ± 22 ng/ml (range, 6−86 ng/ml) compared to normal values (8 ± 2 ng/ml) and 12 of the patients also had detectable APC:PCI complex levels with a mean value of 11 ±17 ng/ml (range, 5−68 ng/ml). Both APC:inhibitor complexes decreased significantly during heparin treatment.

On the Significance of Antithrombin-III, α2-Macroglobulin, α2-Antiplasmin, Histidine-Rich Glycoprotein, and Protein C in Patients with Acute Myocardial Infarction and Deep Vein Thrombosis

1985 ◽  
Vol 54 (02) ◽  
pp. 503-505 ◽  
Author(s):  
Jørgen Gram ◽  
Jørgen Jespersen
Keyword(s):  
Myocardial Infarction ◽  
Acute Myocardial Infarction ◽  
Deep Vein Thrombosis ◽  
Longitudinal Study ◽  
Protein C ◽  
Antithrombin Iii ◽  
Clinical Signs ◽  
Vein Thrombosis ◽  
Α2 Macroglobulin ◽  
Deep Vein

SummaryIn a longitudinal study the plasma levels of antithrombin-III, α2-macroglobulin, α2-antiplasmin, histidine-rich glycoprotein, and protein C were followed in two groups of patients with acute myocardial infarction (AMI), one with and one without deep vein thrombosis (DVT). None of the sequentially studied periods revealed significant differences between the two groups of patients. However, small but consistently higher levels of histidine-rich glycoprotein in patients with DVT suggested the existence among patients submitted for myocardial infarction of a subgroup with increased thrombophilic potential. It was concluded that the inhibitors studied are of little value as possible indicators of the presence of DVT at early stages of the disease when clinical signs are absent and when antithrombotic prophylaxis should preferably be initiated.

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