Induction of stress proteins in chicken embryo cells by low-level zinc contamination in amino acid-free media

1985 ◽  
Vol 122 (2) ◽  
pp. 205-209 ◽  
Author(s):  
Sandra A. Whelan ◽  
Lawrence E. Hightower
Keyword(s):  
Amino Acid ◽  
Chicken Embryo ◽  
Stress Proteins ◽  
Low Level ◽  
Embryo Cells ◽  
Free Media ◽  
Zinc Contamination

Effectiveness of exogenous DNA transfer to chicken embryo cells in vitro and in vivo using retroviral vectors

2007 ◽  
Vol 33 (3) ◽  
pp. 180-182 ◽  
Author(s):  
N. A. Volkova ◽  
A. O. Tulyakova ◽  
L. A. Volkova ◽  
N. A. Zinov’eva ◽  
L. K. Ernst ◽  
...  
Keyword(s):  
Chicken Embryo ◽  
Retroviral Vectors ◽  
Dna Transfer ◽  
Exogenous Dna ◽  
Embryo Cells

scholarly journals Cytoplasmic and Periplasmic Proteomic Signatures of Exponentially Growing Cells of the Psychrophilic BacteriumPseudoalteromonas haloplanktisTAC125

2010 ◽  
Vol 77 (4) ◽  
pp. 1276-1283 ◽  
Author(s):  
Boris Wilmes ◽  
Holger Kock ◽  
Susanne Glagla ◽  
Dirk Albrecht ◽  
Birgit Voigt ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Amino Acid ◽  
Growth Rates ◽  
Stress Proteins ◽  
High Capacity ◽  
Tca Cycle ◽  
Fast Growth ◽  
Natural Environments ◽  
Permissive Temperature ◽  
Moderate Growth

ABSTRACTThe psychrophilic model bacteriumPseudoalteromonas haloplanktisis characterized by remarkably fast growth rates under low-temperature conditions in a range from 5°C to 20°C. In this study the proteome of cellular compartments, the cytoplasm and periplasm, ofP. haloplanktisstrain TAC125 was analyzed under exponential growth conditions at a permissive temperature of 16°C. By means of two-dimensional protein gel electrophoresis and mass spectrometry, a first inventory of the most abundant cytoplasmic and periplasmic proteins expressed in a peptone-supplemented minimal medium was established. By this approach major enzymes of the amino acid catabolism of this marine bacterium could be functionally deduced. The cytoplasmic proteome showed a predominance of amino acid degradation pathways and tricarboxylic acid (TCA) cycle enzymes but also the protein synthesis machinery. Furthermore, high levels of cold acclimation and oxidative stress proteins could be detected at this moderate growth temperature. The periplasmic proteome was characterized by a significant abundance of transporters, especially of highly expressed putative TonB-dependent receptors. This high capacity for protein synthesis, efficient amino acid utilization, and substrate transport may contribute to the fast growth rates of the copiotrophic bacteriumP. haloplanktisin its natural environments.

scholarly journals Preparation of genetically engineered A/H5N1 and A/H7N1 pandemic vaccine viruses by reverse genetics in a mixture of Vero and chicken embryo cells

2007 ◽  
Vol 1 (3) ◽  
pp. 95-104 ◽  
Author(s):  
Isabelle Legastelois ◽  
Adolfo Garcia-Sastre ◽  
Peter Palese ◽  
Terrence M. Tumpey ◽  
Taronna R. Maines ◽  
...  
Keyword(s):  
Chicken Embryo ◽  
Reverse Genetics ◽  
Genetically Engineered ◽  
Embryo Cells ◽  
Pandemic Vaccine
Sign in / Sign up

Export Citation Format

Share Document