Characterization of neutral and cationic amino acid transport inXenopus oocytes

1989 ◽  
Vol 141 (3) ◽  
pp. 645-652 ◽  
Author(s):  
Michael J. Campa ◽  
Michael S. Kilberg
Keyword(s):  
Amino Acid ◽  
Amino Acid Transport ◽  
Acid Transport ◽  
Cationic Amino Acid

Multiple pathways for cationic amino acid transport in rat thyroid epithelial cell line PC Cl3

2005 ◽  
Vol 288 (2) ◽  
pp. C290-C303 ◽  
Author(s):  
Tiziano Verri ◽  
Cinzia Dimitri ◽  
Sonia Treglia ◽  
Fabio Storelli ◽  
Stefania De Micheli ◽  
...  
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Cell Line ◽  
Amino Acid Transport ◽  
Transport Systems ◽  
Residual Fraction ◽  
Acid Transport ◽  
Thyroid Cells ◽  
Cationic Amino Acid ◽  
Rat Thyroid

Information regarding cationic amino acid transport systems in thyroid is limited to Northern blot detection of y+LAT1 mRNA in the mouse. This study investigated cationic amino acid transport in PC cell line clone 3 (PC Cl3 cells), a thyroid follicular cell line derived from a normal Fisher rat retaining many features of normal differentiated follicular thyroid cells. We provide evidence that in PC Cl3 cells plasmalemmal transport of cationic amino acids is Na+ independent and occurs, besides diffusion, with the contribution of high-affinity, carrier-mediated processes. Carrier-mediated transport is via y+, y+L, and b0,+ systems, as assessed by l-arginine uptake and kinetics, inhibition of l-arginine transport by N-ethylmaleimide and neutral amino acids, and l-cystine transport studies. y+L and y+ systems account for the highest transport rate (with y+L > y+) and b0,+ for a residual fraction of the transport. Uptake data correlate to expression of the genes encoding for CAT-1, CAT-2B, 4F2hc, y+LAT1, y+LAT2, rBAT, and b0,+AT, an expression profile that is also shown by the rat thyroid gland. In PC Cl3 cells cationic amino acid uptake is under TSH and/or cAMP control (with transport increasing with increasing TSH concentration), and upregulation of CAT-1, CAT-2B, 4F2hc/y+LAT1, and rBAT/b0,+AT occurs at the mRNA level under TSH stimulation. Our results provide the first description of an expression pattern of cationic amino acid transport systems in thyroid cells. Furthermore, we provide evidence that extracellular l-arginine is a crucial requirement for normal PC Cl3 cell growth and that long-term l-arginine deprivation negatively influences CAT-2B expression, as it correlates to reduction of CAT-2B mRNA levels.

Amino acid transport in thermophiles: characterization of an arginine-binding protein in Thermotoga maritima

2009 ◽  
Vol 6 (1) ◽  
pp. 142-151 ◽  
Author(s):  
Matthew S. Luchansky ◽  
Bryan S. Der ◽  
Sabato D’Auria ◽  
Gabriella Pocsfalvi ◽  
Luisa Iozzino ◽  
...  
Keyword(s):  
Amino Acid ◽  
Amino Acid Transport ◽  
Thermotoga Maritima ◽  
Binding Protein ◽  
Acid Transport

Regulation and characterization of two inducible amino-acid transport systems in Chlorella vulgaris

Planta ◽  
1983 ◽  
Vol 159 (5) ◽  
pp. 404-410 ◽  
Author(s):  
Norbert Sauer ◽  
Ewald Komor ◽  
Widmar Tanner
Keyword(s):  
Amino Acid ◽  
Chlorella Vulgaris ◽  
Amino Acid Transport ◽  
Transport Systems ◽  
Acid Transport

scholarly journals Analysis of Cationic Amino Acid Transport Activity in Canine Lens Epithelial Cells

2013 ◽  
Vol 62 (4) ◽  
pp. 311-317 ◽  
Author(s):  
Hideharu Ochiai ◽  
Jun Moriyama ◽  
Nobuyuki Kanemaki ◽  
Reiichiro Sato ◽  
Ken Onda
Keyword(s):  
Amino Acid ◽  
Epithelial Cells ◽  
Amino Acid Transport ◽  
Lens Epithelial Cells ◽  
Acid Transport ◽  
Transport Activity ◽  
Cationic Amino Acid

The Characterization of Intestinal Acidic Amino-Acid Transport

1985 ◽  
Vol 456 (1 Membrane Tran) ◽  
pp. 80-82 ◽  
Author(s):  
THERESA G. WINGROVE ◽  
GEORGE A. KIMMICH
Keyword(s):  
Amino Acid ◽  
Amino Acid Transport ◽  
Acid Transport ◽  
Acidic Amino Acid
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