RGS1 silencing inhibits the inflammatory response and angiogenesis in rheumatoid arthritis rats through the inactivation of Toll‐like receptor signaling pathway

2019 ◽  
Vol 234 (11) ◽  
pp. 20432-20442 ◽  
Author(s):  
Xumin Hu ◽  
Jianhua Tang ◽  
Gang Zeng ◽  
Xuyun Hu ◽  
Peng Bao ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Inflammatory Response ◽  
Signaling Pathway ◽  
Receptor Signaling ◽  
Toll Like Receptor ◽  
Receptor Signaling Pathway

scholarly journals Network Pharmacology-Based Study of Active Ingredients and Mechanisms of Compound Xuanju for Rheumatoid Arthritis

2020 ◽  
Vol 3 (10) ◽  
pp. 712-723
Author(s):  
Fuxue Meng ◽  
Xiaomai Tao ◽  
Longkuan Li ◽  
Xin Yang
Keyword(s):  
Rheumatoid Arthritis ◽  
Signaling Pathway ◽  
Akt Signaling ◽  
Venn Diagram ◽  
Receptor Signaling ◽  
Toll Like Receptor ◽  
Akt Signaling Pathway ◽  
Active Ingredients ◽  
Receptor Signaling Pathway ◽  
Target Database

Objective: Compound Xuanju has good effects in treating rheumatoid arthritis (RA), but its composition is complex, and its active ingredients and mechanism have not been fully defined. In this study, the active ingredients and mechanism of compound Xuanju for the treatment of RA were explored through network pharmacological methods. Methods: TCMSP and TCMID, Pubmed, CNKI, Wanfang, and VIP databases were used to screen, select active pharmaceutical ingredients and targets; Drugbank disease target screening database, GeneCards database, Therapeutic The Target Database (TTD) database and DisGeNET database were used to collect RA  targets, and OmicShare was used to screen compound Xuanju and RA for common targets and construct a Venn diagram. A protein target database String was used to construct a common target interaction network. OmicShare mapping software builds a "drug-active ingredient-target" network and analyzes their associations. DAVID online software performs gene annotation (GO) and KEGG pathway enrichment analysis on key targets. Results: A total of 73 effective ingredients of compound Xuanju were obtained, and corresponding to 229 targets; 2337 targets for RA. 155 key targets for potential active ingredients of compound Xuanju predicted therapeutic effect of RA, the key targets map 55 active ingredients of compound Xuanju capsules. These targets mainly involve signaling pathways such as Toll-like receptor signaling pathway, PI3K-Akt signaling pathway, HIF-1 signaling pathway, and TNF signaling pathway acting on RA. Conclusion: Compound Xuanju may via its potential 55 active ingredients act on 155 targets to treat RA through Toll-like receptor signaling pathway, PI3K-Akt signaling pathway, NF-κB signaling pathway, HIF-1 signaling pathway and TNF signaling pathway. This study lays the theoretical basis for the widespread application of compound Xuanju in clinical practice.

scholarly journals Transcriptome Analysis for Genes Associated with Small Ruminant Lentiviruses Infection in Goats of Carpathian Breed

Viruses ◽  
2021 ◽  
Vol 13 (10) ◽  
pp. 2054
Author(s):  
Monika Olech ◽  
Katarzyna Ropka-Molik ◽  
Tomasz Szmatoła ◽  
Katarzyna Piórkowska ◽  
Jacek Kuźmak
Keyword(s):  
Gene Expression ◽  
Immune Response ◽  
Signaling Pathways ◽  
Signaling Pathway ◽  
Proviral Load ◽  
Cytokine Production ◽  
Small Ruminant ◽  
Receptor Signaling ◽  
Toll Like Receptor ◽  
Receptor Signaling Pathway

Small ruminant lentiviruses (SRLV) are economically important viral pathogens of sheep and goats. SRLV infection may interfere in the innate and adaptive immunity of the host, and genes associated with resistance or susceptibility to infection with SRLV have not been fully recognized. The presence of animals with relatively high and low proviral load suggests that some host factors are involved in the control of virus replication. To better understand the role of the genes involved in the host response to SRLV infection, RNA sequencing (RNA-seq) method was used to compare whole gene expression profiles in goats carrying both a high (HPL) and low (LPL) proviral load of SRLV and uninfected animals. Data enabled the identification of 1130 significant differentially expressed genes (DEGs) between control and LPL groups: 411 between control and HPL groups and 1434 DEGs between HPL and LPL groups. DEGs detected between the control group and groups with a proviral load were found to be significantly enriched in several gene ontology (GO) terms, including an integral component of membrane, extracellular region, response to growth factor, inflammatory and innate immune response, transmembrane signaling receptor activity, myeloid differentiation primary response gene 88 (MyD88)-dependent toll-like receptor signaling pathway as well as regulation of cytokine secretion. Our results also demonstrated significant deregulation of selected pathways in response to viral infection. The presence of SRLV proviral load in blood resulted in the modification of gene expression belonging to the toll-like receptor signaling pathway, the tumor necrosis factor (TNF) signaling pathway, the cytokine-cytokine receptor interaction, the phagosome, the Ras signaling pathway, the phosphatidylinositol 3-kinase (PI3K)/protein kinase B (AKT) (PI3K-Akt) signaling pathway and rheumatoid arthritis. It is worth mentioning that the most predominant in all pathways were genes represented by toll-like receptors, tubulins, growth factors as well as interferon gamma receptors. DEGs detected between LPL and HPL groups were found to have significantly enriched regulation of signaling receptor activity, the response to toxic substances, nicotinamide adenine dinucleotide (NADH) dehydrogenase complex assembly, cytokine production, vesicle, and vacuole organization. In turn, the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway tool classified DEGs that enrich molecular processes such as B and T-cell receptor signaling pathways, natural killer cell-mediated cytotoxicity, Fc gamma R-mediated phagocytosis, toll-like receptor signaling pathways, TNF, mammalian target of rapamycin (mTOR) signaling and forkhead box O (Foxo) signaling pathways, etc. Our data indicate that changes in SRLV proviral load induced altered expression of genes related to different biological processes such as immune response, inflammation, cell locomotion, and cytokine production. These findings provide significant insights into defense mechanisms against SRLV infection. Furthermore, these data can be useful to develop strategies against SRLV infection by selection of animals with reduced SRLV proviral concentration that may lead to a reduction in the spread of the virus.

Negative regulation of Toll-like receptor signaling pathway

2009 ◽  
Vol 11 (3) ◽  
pp. 321-327 ◽  
Author(s):  
Jie Wang ◽  
Yu Hu ◽  
Wei Wen Deng ◽  
Bing Sun
Keyword(s):  
Signaling Pathway ◽  
Negative Regulation ◽  
Receptor Signaling ◽  
Toll Like Receptor ◽  
Receptor Signaling Pathway

Distinct evolution of toll-like receptor signaling pathway genes in cetaceans

2019 ◽  
Vol 41 (12) ◽  
pp. 1417-1430
Author(s):  
Ran Tian ◽  
Inge Seim ◽  
Zepeng Zhang ◽  
Ying Yang ◽  
Wenhua Ren ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Receptor Signaling ◽  
Toll Like Receptor ◽  
Receptor Signaling Pathway

scholarly journals S6K1 Negatively Regulates TAK1 Activity in the Toll-Like Receptor Signaling Pathway

2013 ◽  
Vol 34 (3) ◽  
pp. 510-521 ◽  
Author(s):  
S. Y. Kim ◽  
K.-H. Baik ◽  
K.-H. Baek ◽  
K.-H. Chah ◽  
K. A. Kim ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Receptor Signaling ◽  
Toll Like Receptor ◽  
Receptor Signaling Pathway
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