Early and natural embryonic death in Lagostomus maximus : Association with the uterine glands, vasculature, and musculature

2020 ◽  
Vol 281 (7) ◽  
pp. 710-724
Author(s):  
Francisco Acuña ◽  
Claudio G. Barbeito ◽  
Enrique L. Portiansky ◽  
Guadalupe Ranea ◽  
Fabian Nishida ◽  
...  
Keyword(s):  
Lagostomus Maximus ◽  
Uterine Glands ◽  
Embryonic Death

Role of the uterine glands in the early embryonic death of mammals using a natural model: the plains viscacha Lagostomus maximus (Rodentia, Chinchillidae)

Placenta ◽  
2019 ◽  
Vol 83 ◽  
pp. e29
Author(s):  
Francisco Acuña ◽  
Claudio Gustavo Barbeito ◽  
Fabián Nishida ◽  
Enrique Leo Portiansky ◽  
María Angelica Miglino ◽  
...  
Keyword(s):  
Lagostomus Maximus ◽  
Uterine Glands ◽  
Embryonic Death

Prenatal development in Lagostomus maximus (Rodentia, Chinchillidae): A unique case among eutherian mammals of physiological embryonic death

2021 ◽  
Vol 282 (5) ◽  
pp. 720-732
Author(s):  
Francisco Acuña ◽  
Claudio G. Barbeito ◽  
Enrique L. Portiansky ◽  
María A. Miglino ◽  
Mirta A. Flamini
Keyword(s):  
Prenatal Development ◽  
Unique Case ◽  
Lagostomus Maximus ◽  
Embryonic Death

Natural embryonic death in the plain viscacha Lagostomus maximus: are there previous anatomo-histological variations that would intervene in the differential survival of the implantations?

Placenta ◽  
2019 ◽  
Vol 83 ◽  
pp. e25
Author(s):  
Francisco Acuña ◽  
Claudio Gustavo Barbeito ◽  
Guadalupe Ranea ◽  
Enrique Leo Portiansky ◽  
María Angélica Miglino ◽  
...  
Keyword(s):  
Differential Survival ◽  
Lagostomus Maximus ◽  
Embryonic Death

Two Novel Cases of Autosomal Translocations in the Horse: Warmblood Family Segregating t(4;30) and a Cloned Arabian with a de novo t(12;25)

2020 ◽  
Vol 160 (11-12) ◽  
pp. 688-697
Author(s):  
Sharmila Ghosh ◽  
Candice F. Carden ◽  
Rytis Juras ◽  
Mayra N. Mendoza ◽  
Matthew J. Jevit ◽  
...  
Keyword(s):  
Somatic Cell Nuclear Transfer ◽  
Nuclear Transfer ◽  
Congenital Abnormalities ◽  
Assisted Reproductive Technologies ◽  
De Novo ◽  
Reproductive Technologies ◽  
Balanced Translocation ◽  
Translocation Breakpoints ◽  
Normal Offspring ◽  
Embryonic Death

We report 2 novel autosomal translocations in the horse. In Case 1, a breeding stallion with a balanced t(4p;30) had produced normal foals and those with congenital abnormalities. Of his 9 phenotypically normal offspring, 4 had normal karyotypes, 4 had balanced t(4p;30), and 1 carried an unbalanced translocation with tertiary trisomy of 4p. We argue that unbalanced forms of t(4p;30) are more tolerated and result in viable congenital abnormalities, without causing embryonic death like all other known equine autosomal translocations. In Case 2, two stallions produced by somatic cell nuclear transfer from the same donor were karyotyped because of fertility issues. A balanced translocation t(12q;25) was found in one, but not in the other clone. The findings underscore the importance of routine cytogenetic screening of breeding animals and animals produced by assisted reproductive technologies. These cases will contribute to molecular studies of translocation breakpoints and their genetic consequences in the horse.

scholarly journals Overfeeding Extends the Period of Annual Cyclicity but Increases the Risk of Early Embryonic Death in Shetland Pony Mares

Animals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 361
Author(s):  
Nicky M. M. D’Fonseca ◽  
Charlotte M. E. Gibson ◽  
Iris Hummel ◽  
David A. van Doorn ◽  
Ellen Roelfsema ◽  
...  
Keyword(s):  
Embryo Transfer ◽  
Reproductive Activity ◽  
High Energy ◽  
Morphometric Parameters ◽  
Net Energy ◽  
Conceptus Development ◽  
And Control ◽  
Annual Duration ◽  
Embryonic Death

Obesity has been associated with altered reproductive activity in mares, and may negatively affect fertility. To examine the influence of long-term high-energy (HE) feeding on fertility, Shetland pony mares were fed a diet containing 200% of net energy (NE) requirements during a three-year study. The incidence of hemorrhagic anovulatory follicles (HAF) and annual duration of cyclicity were compared to those in control mares receiving a maintenance diet. Day-7 embryos were flushed and transferred between donor and recipient mares from both groups; the resulting conceptuses were collected 21 days after transfer to assess conceptus development. HE mares became obese, and embryos recovered from HE mares were more likely to succumb to early embryonic death. The period of annual cyclicity was extended in HE compared to control mares in all years. The incidence of HAFs did not consistently differ between HE and control mares. No differences in embryo morphometric parameters were apparent. In conclusion, consuming a HE diet extended the duration of cyclicity, and appeared to increase the likelihood of embryos undergoing early embryonic death following embryo transfer.

scholarly journals Recombinase-Aided Amplification Coupled with Lateral Flow Dipstick for Efficient and Accurate Detection of Porcine Parvovirus

Life ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 762
Author(s):  
Yihong He ◽  
Wenxian Chen ◽  
Jindai Fan ◽  
Shuangqi Fan ◽  
Hongxing Ding ◽  
...  
Keyword(s):  
Detection Method ◽  
Cross Reactivity ◽  
Lateral Flow ◽  
Porcine Parvovirus ◽  
Swine Industry ◽  
Resource Limited ◽  
Efficient Detection ◽  
Lateral Flow Dipstick ◽  
Polymerase Chain ◽  
Embryonic Death

Porcine parvovirus (PPV) infection is the primary cause of SMEDI (stillbirth; mummification; embryonic death; infertility) syndrome, which is a global burden for the swine industry. Thus, it is crucial to establish a rapid and efficient detection method against PPV infection. In the present work, we developed a recombinase-aided amplification (RAA) assay, coupled with a lateral flow dipstick (LFD), to achieve an amplification of PPV DNA at 37 °C within 15 min. The detection limits of PPV RAA-LFD assay were 102 copies/μL recombinant plasmid pMD19-T-VP1, 6.38 × 10−7 ng/μL PPV DNA, and 10−1 TCID50/mL virus, respectively. This method was highly specific for PPV detection with no cross-reactivity for other swine pathogens. In contrast to polymerase chain reaction (PCR), the PPV RAA-LFD assay is more sensitive and cost-saving. Hence, the established PPV RAA-LFD assay provided an alternative for PPV detection, especially in resource-limited regions.

scholarly journals Forkhead box a2 (FOXA2) is essential for uterine function and fertility

2017 ◽  
Vol 114 (6) ◽  
pp. E1018-E1026 ◽  
Author(s):  
Andrew M. Kelleher ◽  
Wang Peng ◽  
James K. Pru ◽  
Cindy A. Pru ◽  
Francesco J. DeMayo ◽  
...  
Keyword(s):  
Stromal Cell ◽  
Adult Mouse ◽  
Embryo Implantation ◽  
Critical Event ◽  
Mouse Uterus ◽  
Forkhead Box ◽  
Uterine Gland ◽  
Blastocyst Implantation ◽  
Uterine Function ◽  
Uterine Glands

Establishment of pregnancy is a critical event, and failure of embryo implantation and stromal decidualization in the uterus contribute to significant numbers of pregnancy losses in women. Glands of the uterus are essential for establishment of pregnancy in mice and likely in humans. Forkhead box a2 (FOXA2) is a transcription factor expressed specifically in the glands of the uterus and is a critical regulator of postnatal uterine gland differentiation in mice. In this study, we conditionally deleted FOXA2 in the adult mouse uterus using the lactotransferrin Cre (Ltf-Cre) model and in the neonatal mouse uterus using the progesterone receptor Cre (Pgr-Cre) model. The uteri of adult FOXA2-deleted mice were morphologically normal and contained glands, whereas the uteri of neonatal FOXA2-deleted mice were completely aglandular. Notably, adult FOXA2-deleted mice are completely infertile because of defects in blastocyst implantation and stromal cell decidualization. Leukemia inhibitory factor (LIF), a critical implantation factor of uterine gland origin, was not expressed during early pregnancy in adult FOXA2-deleted mice. Intriguingly, i.p. injections of LIF initiated blastocyst implantation in the uteri of both gland-containing and glandless adult FOXA2-deleted mice. Although pregnancy was rescued by LIF and was maintained to term in uterine gland-containing adult FOXA2-deleted mice, pregnancy failed by day 10 in neonatal FOXA2-deleted mice lacking uterine glands. These studies reveal a previously unrecognized role for FOXA2 in regulation of adult uterine function and fertility and provide original evidence that uterine glands and, by inference, their secretions play important roles in blastocyst implantation and stromal cell decidualization.

scholarly journals First record of an infection by tissue cyst-forming coccidia in wild vizcachas (Lagostomus maximus, Rodentia) of Argentina

2021 ◽  
Author(s):  
Ruth Cwirenbaum ◽  
Alejandro R. Schmidt ◽  
Santiago A. Cortasa ◽  
María C. Corso ◽  
Alfredo D. Vitullo ◽  
...  
Keyword(s):  
First Record ◽  
Tissue Cyst ◽  
Lagostomus Maximus
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