Hepatocyte growth factor stimulates cell motility in cultures of the striatal progenitor cells ST14A

Abstract Bone marrow (BM) stromal cells, which include macrophages, fibroblasts, endothelial cells, and adipocytes, have been shown to produce several factors that modulate the growth of BM progenitors. Hepatocyte growth factor (HGF) is a fibroblast-derived factor and has recently been shown to be a ligand for the c-met proto-oncogene, a member of the receptor class of tyrosine kinases. c-met messenger RNA (mRNA) is predominantly expressed in epithelial cells, but has been detected in several murine hematopoietic progenitor cell lines, suggesting that HGF and met might function during hematopoiesis. Here, BM cells were found to express both met mRNA and protein. Moreover, HGF was shown to synergize with interleukin-3 and granulocyte-macrophage colony-stimulating factor to stimulate colony formation of hematopoietic progenitor cells in vitro. These results show that, in addition to its activity on epithelial cells, HGF is a new member of the functionally related group of factors that modulate hematopoiesis.

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Comparison of biological and immunochemical properties indicates that scatter factor and hepatocyte growth factor are indistinguishable

Journal of Cell Science ◽

10.1242/jcs.100.1.173 ◽

1991 ◽

Vol 100 (1) ◽

pp. 173-177 ◽

Cited By ~ 5

Author(s):

R.A. Furlong ◽

T. Takehara ◽

W.G. Taylor ◽

T. Nakamura ◽

J.S. Rubin

Keyword(s):

Growth Factor ◽

Hepatocyte Growth Factor ◽

Cell Motility ◽

Recombinant Proteins ◽

Sequence Data ◽

Biological Activities ◽

Scatter Factor ◽

Naturally Occurring ◽

Biological Studies ◽

Hepatocyte Growth

Scatter factor, a stimulant of epithelial cell motility, and Hepatocyte Growth Factor (HGF) were compared by cross-biological studies using naturally occurring and recombinant proteins in four bioassays. Both scatter factor and HGF produced similar effects in cell motility and DNA synthesis assays. Antibodies to scatter factor or HGF neutralized the biological activities of each cytokine, and in immunoblotting reacted with species of the same Mr. These results, together with the available sequence data, suggest that scatter factor and HGF are the same protein.

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Differential regulation of hepatocyte growth factor/scatter factor by cell surface proteoglycans and free glycosaminoglycan chains

Journal of Cell Science ◽

10.1242/jcs.112.12.1999 ◽

1999 ◽

Vol 112 (12) ◽

pp. 1999-2009 ◽

Cited By ~ 1

Author(s):

J.A. Deakin ◽

M. Lyon

Keyword(s):

Growth Factor ◽

Cell Surface ◽

Hepatocyte Growth Factor ◽

Cell Motility ◽

Heparan Sulphate ◽

Mitogen Activated Protein Kinase ◽

Dermatan Sulphate ◽

Mitogen Activated Protein ◽

Hepatocyte Growth ◽

Darby Canine Kidney

Hepatocyte growth factor interacts with both heparan and dermatan sulphates, in addition to its specific signalling receptor, Met. However, the extent of glycosaminoglycan involvement in its biological activity remains uncertain. We have investigated the effects of exogenous glycosaminoglycan addition upon hepatocyte growth factor-stimulated motility of Madin-Darby canine kidney cells. Exogenous heparan/dermatan sulphate chains behave similarly as either potentiators or inhibitors of cell motility (depending upon the assay). Specific heparan sulphate oligosaccharides, of octasaccharide or larger, elicit similar effects, though with reduced potency. Additionally we have investigated the motility of cells made completely deficient in functional proteoglycans by metabolic inhibition of glycosaminoglycan sulphation, using chlorate. Such cells are completely unresponsive to hepatocyte growth factor, both in terms of downstream phosphorylation of mitogen-activated protein kinase and actual cell motility, though they do remain responsive to phorbol ester. Interestingly, although cell responsiveness to hepatocyte growth factor is not restored by exogenous heparan/dermatan sulphate chains, it is by an immobilised heparan sulphate proteoglycan substratum. These findings suggest that hepatocyte growth factor activity is not only critically dependent upon the presence of glycosaminoglycan, but specifically requires an intact proteoglycan structure located in close apposition to cell surface Met.

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Intrinsic, extrinsic and environmental regulation of muscle satellite cell motility

10.32469/10355/46091 ◽

2011 ◽

Author(s):

◽

Ashley Lynn Siegal

Keyword(s):

Growth Factor ◽

Hepatocyte Growth Factor ◽

Cell Motility ◽

Satellite Cell ◽

Satellite Cells ◽

Tibialis Anterior Muscle ◽

Repulsive Interaction ◽

Myogenic Stem Cells ◽

Hepatocyte Growth

[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT REQUEST OF AUTHOR.] Skeletal muscle repair and regeneration requires the activity of satellite cells, a population of myogenic stem cells. Previously, little data existed on the motility of satellite cells a critical component to tissue repair. Using timelapse videomicroscopy to assess satellite cell motility on the surface of single living myofibers, we have identified a requirement for the laminin-binding integrin a7b1 in satellite cell motility, as well as a role for hepatocyte growth factor in promoting directional persistence. We also observed more persistent long-term contact, potential cell-cell attractive and repulsive interaction, and migration between host myofibers. We found that satellite cells express multiple members of each of the four major families of guidance molecules. Satellite cell migration in vivo may be more extensive than currently thought, and could be regulated by combinations of signals including adhesive haptotaxis, soluble factors, and guidance cues. CXCL12/SDF-1 and hepatocyte growth factor/scatter factor (HGF) are included in these released factors satellite cell displacement and velocity and chemotaxis were quantified. Purified HGF and SDF-1a were injected into the Tibialis Anterior muscle (TA) to test the sufficiency of these factors for satellite cell movement in vivo. A better understanding of how satellite cells actually respond to an injury in a healthy muscle and if they are mobilized and motile from a distance will be critical to knowing if they can be induced to move through damaged or diseased muscle tissue.

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