scholarly journals High-throughput screening of PLGA thin films utilizing hydrophobic fluorescent dyes for hydrophobic drug compounds

2011 ◽  
Vol 100 (10) ◽  
pp. 4317-4329 ◽  
Author(s):  
Terry W.J. Steele ◽  
Charlotte L. Huang ◽  
Saranya Kumar ◽  
Effendi Widjaja ◽  
Freddy Yin Chiang Boey ◽  
...  
Keyword(s):  
Thin Films ◽  
High Throughput ◽  
High Throughput Screening ◽  
Fluorescent Dyes ◽  
Hydrophobic Drug ◽  
Drug Compounds

scholarly journals High Throughput Screening of Valganciclovir in Acidic Microenvironments of Polyester Thin Films

Materials ◽  
2015 ◽  
Vol 8 (4) ◽  
pp. 1714-1728 ◽  
Author(s):  
Teilo Schaller ◽  
Tobias Wenner ◽  
Rupesh Agrawal ◽  
Stephen Teoh ◽  
Li Phua ◽  
...  
Keyword(s):  
Thin Films ◽  
High Throughput ◽  
High Throughput Screening

scholarly journals Involvement of multiple influx and efflux transporters in the accumulation of cationic fluorescent dyes byEscherichia coli

2019 ◽  
Author(s):  
Srijan Jindal ◽  
Lei Yang ◽  
Philip J. Day ◽  
Douglas B. Kell
Keyword(s):  
Steady State ◽  
High Throughput ◽  
High Throughput Screening ◽  
Fluorescent Dyes ◽  
Gene Knockout ◽  
Sybr Green ◽  
Efflux Transporters ◽  
General Strategy ◽  
Individual Gene

AbstractWe used high-throughput flow cytometry to assess the ability of individual gene knockout strains ofE colito take up two membrane-permeable, cationic fluorescent dyes, viz the carbocyanine diS-C3(5) and the DNA dye SYBR Green. Individual strains showed a large range of distributions of uptake. The range of modal steady-state uptakes for the carbocyanine between the different strains was 36-fold. Knockouts of the ATP synthase α- and β-subunits greatly inhibited uptake, implying that most uptake was ATP-driven rather than being driven by say a membrane potential. Dozens of transporters changed the steady-state uptake of the dye by more than 50% with respect to that of the wild type, in both directions (increased or decreased); knockouts in known influx and efflux transporters behaved as expected, giving confidence in the general strategy. Many of the knockouts with the most reduced uptake were transporter genes of unknown function (‘y-genes’). Similarly, several overexpression variants in the ‘ASKA’ collection had the anticipated, opposite effects. Similar findings were made with SYBR Green (the range being some 69-fold), though despite it too containing a benzimidazole motif there was negligible correlation between its uptake and that of the carbocyanine when compared across the various strains. Overall, we conclude that the uptake of these dyes may be catalysed by a great many transporters of possibly broad and presently unknown specificity. This casts serious doubt upon the use of such dyes as quantitative stains for representing either bioenergetic parameters or the amount of cellular DNA in unfixed cells (in vivo). By contrast, it opens up their potential use as transporter assay substrates in high-throughput screening.

High-Throughput Screening of Antisolvents for the Deposition of High-Quality Perovskite Thin Films

2020 ◽  
Vol 12 (23) ◽  
pp. 26026-26032
Author(s):  
Joseph G. Manion ◽  
Andrew H. Proppe ◽  
Garion E. J. Hicks ◽  
Edward H. Sargent ◽  
Dwight S. Seferos
Keyword(s):  
Thin Films ◽  
High Throughput ◽  
High Throughput Screening ◽  
High Quality

Interference: A Much-Neglected Aspect in High-Throughput Screening of Nanoparticles

2020 ◽  
Vol 39 (5) ◽  
pp. 397-421
Author(s):  
Charlene Andraos ◽  
Il Je Yu ◽  
Mary Gulumian
Keyword(s):  
Big Data ◽  
High Throughput ◽  
High Throughput Screening ◽  
Fluorescent Dyes ◽  
Membrane Integrity ◽  
Data Generation ◽  
Label Free ◽  
Predictive Toxicology ◽  
Toxicity Assay ◽  
Novel Concept

Despite several studies addressing nanoparticle (NP) interference with conventional toxicity assay systems, it appears that researchers still rely heavily on these assays, particularly for high-throughput screening (HTS) applications in order to generate “big” data for predictive toxicity approaches. Moreover, researchers often overlook investigating the different types of interference mechanisms as the type is evidently dependent on the type of assay system implemented. The approaches implemented in the literature appear to be not adequate as it often addresses only one type of interference mechanism with the exclusion of others. For example, interference of NPs that have entered cells would require intracellular assessment of their interference with fluorescent dyes, which has so far been neglected. The present study investigated the mechanisms of interference of gold NPs and silver NPs in assay systems implemented in HTS including optical interference as well as adsorption or catalysis. The conventional assays selected cover all optical read-out systems, that is, absorbance (XTT toxicity assay), fluorescence (CytoTox-ONE Homogeneous membrane integrity assay), and luminescence (CellTiter Glo luminescent assay). Furthermore, this study demonstrated NP quenching of fluorescent dyes also used in HTS (2′,7′-dichlorofluorescein, propidium iodide, and 5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethyl-benzamidazolocarbocyanin iodide). To conclude, NP interference is, as such, not a novel concept, however, ignoring this aspect in HTS may jeopardize attempts in predictive toxicology. It should be mandatory to report the assessment of all mechanisms of interference within HTS, as well as to confirm results with label-free methodologies to ensure reliable big data generation for predictive toxicology.

scholarly journals Real-Time Apoptosis and Viability High-Throughput Screening of 3D Multicellular Tumor Spheroids Using the Celigo Image Cytometer

2017 ◽  
Vol 23 (2) ◽  
pp. 202-210 ◽  
Author(s):  
Sarah Kessel ◽  
Scott Cribbes ◽  
Surekha Bonasu ◽  
Jean Qiu ◽  
Leo Li-Ying Chan
Keyword(s):  
Real Time ◽  
High Throughput ◽  
High Throughput Screening ◽  
Cancer Drug ◽  
Tumor Spheroid ◽  
Potential Drug ◽  
Multicellular Tumor Spheroid ◽  
Drug Candidates ◽  
Secondary Screen ◽  
Drug Compounds

Three-dimensional tumor spheroid models have been increasingly used to investigate and characterize cancer drug compounds. Previously, the Celigo image cytometer has demonstrated its utility in a high-throughput screening manner for evaluating potential drug candidates in a 3D multicellular tumor spheroid (MCTS) primary screen. In addition, we have developed real-time kinetic caspase 3/7 apoptosis and propidium iodide viability 3D MCTS assays, both of which can be used in a secondary screen to better characterize the hit compounds. In this work, we monitored the kinetic apoptotic and cytotoxic effects of 14 compounds in 3D MCTS produced from the glioblastoma cell line U87MG in 384-well plates for 9 days. The kinetic results allowed the categorization of the effects from 14 drug compounds into early and late cytotoxic, apoptotic, cytostatic, and no effects. The real-time apoptosis and viability screening method can serve as an improved secondary screen to better understand the mechanism of action of these potential drug candidates identified from the primary screen, allowing one to identify a more qualified drug candidate and streamline the drug discovery process of research and development.

Macro-/mesoporous titania thin films: analysing the effect of pore architecture on photocatalytic activity using high-throughput screening

2015 ◽  
Vol 3 (48) ◽  
pp. 24557-24567 ◽  
Author(s):  
Natalita M. Nursam ◽  
Xingdong Wang ◽  
Rachel A. Caruso
Keyword(s):  
Thin Films ◽  
Photocatalytic Activity ◽  
High Throughput ◽  
High Throughput Screening ◽  
Mesoporous Titania ◽  
Pore Architecture ◽  
High Throughput Method

The photocatalytic behaviour of titania thin films was studied using a high-throughput method to correlate the effect of pore and thickness modification.

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