High-Throughput Screening of Antisolvents for the Deposition of High-Quality Perovskite Thin Films

2020 ◽  
Vol 12 (23) ◽  
pp. 26026-26032
Author(s):  
Joseph G. Manion ◽  
Andrew H. Proppe ◽  
Garion E. J. Hicks ◽  
Edward H. Sargent ◽  
Dwight S. Seferos
Keyword(s):  
Thin Films ◽  
High Throughput ◽  
High Throughput Screening ◽  
High Quality

scholarly journals High Throughput Screening of Valganciclovir in Acidic Microenvironments of Polyester Thin Films

Materials ◽  
2015 ◽  
Vol 8 (4) ◽  
pp. 1714-1728 ◽  
Author(s):  
Teilo Schaller ◽  
Tobias Wenner ◽  
Rupesh Agrawal ◽  
Stephen Teoh ◽  
Li Phua ◽  
...  
Keyword(s):  
Thin Films ◽  
High Throughput ◽  
High Throughput Screening

scholarly journals Directed Evolution of Aptamer Discovery Technologies

2021 ◽  
Author(s):  
Diana Wu ◽  
Chelsea Gordon ◽  
John Shin ◽  
Michael Eisenstein ◽  
Hyongsok Tom Soh
Keyword(s):  
High Throughput ◽  
High Throughput Screening ◽  
Click Reaction ◽  
Clinical Diagnostics ◽  
High Quality ◽  
Affinity Reagents ◽  
Wide Range ◽  
Target Binding ◽  
Modified Nucleobases ◽  
Parallel Fashion

Although antibodies are a powerful tool for molecular biology and clinical diagnostics, there are many emerging applications for which nucleic acid-based aptamers can be advantageous. However, generating high-quality aptamers with sufficient affinity and specificity for biomedical applications is a challenging feat for most research laboratories. In this Account, we describe four techniques developed in our lab to accelerate the discovery of high quality aptamer reagents that can achieve robust binding even for challenging molecular targets. The first method is particle display, in which we convert solution-phase aptamers into aptamer particles that can be screened via fluorescence-activated cell sorting (FACS) to quantitatively isolate individual aptamer particles based on their affinity. This enables the efficient isolation of high-affinity aptamers in fewer selection rounds than conventional methods, thereby minimizing selection biases and reducing the emergence of artifacts in the final aptamer pool. We subsequently developed the multi-parametric particle display (MPPD) method, which employs two-color FACS to isolate aptamer particles based on both affinity and specificity, yielding aptamers that exhibit excellent target binding even in complex matrices like serum. The third method is a click chemistry-based particle display (click-PD) that enables the generation and high-throughput screening of non-nattural aptamers with a wide range of base modifications. We have shown that these base-modified aptamers can achieve robust affinity and specificity for targets that have proven challenging or inaccessible with natural nucleotide-based aptamer libraries. Lastly, we describe the non-natural aptamer array (N2A2) platform, in which a modified benchtop sequencing instrument is used to characterize base-modified aptamers in a massively parallel fashion, enabling the efficient identification of molecules with excellent affinity and specificity for their targets. This system first generates aptamer clusters on the flow-cell surface that incorporate alkyne-modified nucleobases, and then performs a click reaction to couple those nucleobases to an azide-modified chemical moiety. This yields a sequence-defined array of tens of millions of base-modified sequences, which can then be characterized in a high-throughput fashion. Collectively, we believe that these advancements are helping to make aptamer technology more accessible, efficient, and robust, thereby enabling the use of these affinity reagents for a wider range of molecular recognition and detection-based applications.

scholarly journals High-throughput screening of PLGA thin films utilizing hydrophobic fluorescent dyes for hydrophobic drug compounds

2011 ◽  
Vol 100 (10) ◽  
pp. 4317-4329 ◽  
Author(s):  
Terry W.J. Steele ◽  
Charlotte L. Huang ◽  
Saranya Kumar ◽  
Effendi Widjaja ◽  
Freddy Yin Chiang Boey ◽  
...  
Keyword(s):  
Thin Films ◽  
High Throughput ◽  
High Throughput Screening ◽  
Fluorescent Dyes ◽  
Hydrophobic Drug ◽  
Drug Compounds

scholarly journals Natural Products in High Throughput Screening: Automated High-Quality Sample Preparation

1999 ◽  
Vol 4 (1) ◽  
pp. 15-25 ◽  
Author(s):  
Ingrid Schmid ◽  
Isabel Sattler ◽  
Susanne Grabley ◽  
Ralf Thiericke
Keyword(s):  
Drug Discovery ◽  
Sample Preparation ◽  
High Throughput ◽  
High Throughput Screening ◽  
Solid Phase ◽  
Structural Diversity ◽  
High Quality ◽  
Synthetic Compound ◽  
Natural Origin ◽  
Compound Libraries

At present, compound libraries from combinatorial chemistry are the major source for high throughput screening (HTS) programs in drug discovery. On the other hand, nature has been proven to be an outstanding source for new and innovative drugs. Secondary metabolites from plants, animals, and microorganisms show a striking structural diversity that supplements chemically synthesized compounds or libraries in drug discovery programs. Unfortunately, extracts from natural sources are usually complex mixtures of compounds, often generated in time-consuming and, for the most part, manual processes. Because quality and quantity of the provided samples play a pivotal role in the success of HTS programs, this poses serious problems. In order to make samples of natural origin competitive with synthetic compound libraries, we devised a novel, automated sample preparation procedure based on solid-phase extraction (SPE). By making use of modified Zymark (Hopkinton, MA) RapidTrace® SPE workstations, we developed an easy-to-handle and effective fractionation method that generates high-quality samples from natural origin, fulfilling the requirements for an integration in high throughput drug discovery programs.

Macro-/mesoporous titania thin films: analysing the effect of pore architecture on photocatalytic activity using high-throughput screening

2015 ◽  
Vol 3 (48) ◽  
pp. 24557-24567 ◽  
Author(s):  
Natalita M. Nursam ◽  
Xingdong Wang ◽  
Rachel A. Caruso
Keyword(s):  
Thin Films ◽  
Photocatalytic Activity ◽  
High Throughput ◽  
High Throughput Screening ◽  
Mesoporous Titania ◽  
Pore Architecture ◽  
High Throughput Method

The photocatalytic behaviour of titania thin films was studied using a high-throughput method to correlate the effect of pore and thickness modification.

High Throughput Screening of the Thermal Stability of Colorants in a Polycarbonate Matrix

2003 ◽  
Vol 804 ◽  
Author(s):  
Donald W. Whisenhunt ◽  
Randall Carter ◽  
Ron Shaffer ◽  
William Bulsiewicz ◽  
William Flanagan
Keyword(s):  
Thin Films ◽  
Thermal Stability ◽  
Polymer Matrix ◽  
High Throughput ◽  
High Throughput Screening ◽  
Visible Spectrum ◽  
Reflectance Spectrometry ◽  
Relative Thermal Stability ◽  
Thermal Stability Of

ABSTRACTThe relative thermal stability of 44 colorants of interest to GE Plastics as polymer colorants was determined. The effects of common (non-colorant) additives on the thermal stability in a polymer matrix were also determined. The measurements were carried out by reflectance spectrometry on thin films of polymers that had been subjected to heating cycles. The change in the visible spectrum was used as an indicator of thermal stability. This paper discusses the method and overall results for the experiments.

Drug Discovery (Lead Identification and High Throughput Screening)

2021 ◽  
pp. 46-50
Author(s):  
Ravi Kumar
Keyword(s):  
Drug Discovery ◽  
High Throughput ◽  
High Throughput Screening ◽  
Clinical Development ◽  
High Quality ◽  
New Drug ◽  
Lead Identification ◽  
Pharmaceutical Industries

In this review we will discuss about the Lead identification, the lead identification is mostly used for the discovery of successful clinical development compound, and it is an essential site for drug discovery. Various important factors that required for discovery a quality leads, such as- Physicochemical, ADME, Biological and PK parameters. These all parameters are required for the identification of high-quality leads. The Combinational chemistry is mostly used for the generation of many compounds in only one process from a mixture. The high throughput screening is suitable for new drug in pharmaceutical industries and it’s mostly used from last two decades.

scholarly journals High-Throughput Mass Spectrometry Screening for Inhibitors of Phosphatidylserine Decarboxylase

2007 ◽  
Vol 12 (5) ◽  
pp. 628-634 ◽  
Author(s):  
Chris D. Forbes ◽  
Joshuaine G. Toth ◽  
Can C. Özbal ◽  
William A. Lamarr ◽  
Jennifer A. Pendleton ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Catalytic Activity ◽  
High Throughput ◽  
High Throughput Screening ◽  
Lipid Synthesis ◽  
High Quality ◽  
Compound Libraries ◽  
Z Value ◽  
Large Compound ◽  
Mass Spectrometry Assay

A high-throughput mass spectrometry assay to measure the catalytic activity of phosphatidylserine decarboxylase (PISD) is described. PISD converts phosphatidylserine to phosphatidylethanolamine during lipid synthesis. Traditional methods of measuring PISD activity are low throughput and unsuitable for the high-throughput screening of large compound libraries. The high-throughput mass spectrometry assay directly measures phosphatidylserine and phosphatidylethanolamine using the RapidFire™ platform at a rate of 1 sample every 7.5 s. The assay is robust, with an average Z′ value of 0.79 from a screen of 9920 compounds. Of 60 compounds selected for confirmation, 54 are active in dose-response studies. The application of high-throughput mass spectrometry permitted a high-quality screen to be performed for an otherwise intractable target. ( Journal of Biomolecular Screening 2007:628-634)

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