Assessment of iron bioavailability from twenty elite late-maturing tropical maize varieties using anin vitro digestion/Caco-2 cell model

2004 ◽  
Vol 84 (10) ◽  
pp. 1202-1206 ◽  
Author(s):  
Sylvester O Oikeh ◽  
Abebe Menkir ◽  
Bussie Maziya-Dixon ◽  
Ross M Welch ◽  
Raymond P Glahn
Keyword(s):  
Cell Model ◽  
Iron Bioavailability ◽  
Tropical Maize ◽  
Maize Varieties

scholarly journals Investigating the Type of Gene Action Conditioning Tolerance to Aluminum (Al) Toxicity in Tropical Maize

2019 ◽  
pp. 1-8
Author(s):  
Victoria Ndeke ◽  
Langa Tembo
Keyword(s):  
Root Length ◽  
Aluminum Toxicity ◽  
Gene Action ◽  
Abiotic Factors ◽  
Al Toxicity ◽  
High Yield ◽  
Small Scale ◽  
Tropical Maize ◽  
Maize Varieties ◽  
Small Scale Farmers

Maize is a third important cereal crop in the world after wheat and rice. In Zambia, it is an important staple crop. Its production is however hampered by both biotic and abiotic factors. Among the abiotic factors, Aluminum (Al) toxicity causes high yield losses and is directly linked to acidic soils. Application of lime can ameliorate this problem, but it is expensive for small scale farmers. Developing maize varieties that are tolerant to Al toxicity is cheaper and feasible for small scale farmers. The purpose of this research was to investigate the type of gene action conditioning tolerance to aluminum toxicity in tropical maize.  Eleven inbred lines were mated in an 8 male (4 moderately tolerant and 4 susceptible) x 3 female (resistant) North Carolina Design II. Results revealed that general combining ability (GCA) effects due to both males and females were highly significant (P≤ 0.001) for root biomass. The shoot length GCA effects due to both male and female respectively were significant (P≤ 0.01). Similarly, the GCA effects due to females and males for root length were significant, P≤ 0.01 and P≤ 0.05 respectively. The genotype CML 511 had the most desirable significant GCA effect value (1.40) for root length among the male lines while CML 538 had the most desirable significant GCA effect value (0.92) among the female lines. The baker’s ratio for root length was found to be 0.49 implying that both additive and non-additive gene action were important in conditioning aluminum toxicity tolerance in tropical maize.

scholarly journals Mapping-by-Sequencing via MutMap Identifies a Mutation in ZmCLE7 Underlying Fasciation in a Newly Developed EMS Mutant Population in an Elite Tropical Maize Inbred

Genes ◽  
2020 ◽  
Vol 11 (3) ◽  
pp. 281 ◽  
Author(s):  
Quan Hong Tran ◽  
Ngoc Hong Bui ◽  
Christian Kappel ◽  
Nga Thi Ngoc Dau ◽  
Loan Thi Nguyen ◽  
...  
Keyword(s):  
Point Mutations ◽  
Gene Discovery ◽  
Forward Genetics ◽  
Tropical Maize ◽  
Mapping Procedure ◽  
The Public ◽  
Rapid Mapping ◽  
Maize Varieties ◽  
Comparable Quality ◽  
Mapping By Sequencing

Induced point mutations are important genetic resources for their ability to create hypo- and hypermorphic alleles that are useful for understanding gene functions and breeding. However, such mutant populations have only been developed for a few temperate maize varieties, mainly B73 and W22, yet no tropical maize inbred lines have been mutagenized and made available to the public to date. We developed a novel Ethyl Methanesulfonate (EMS) induced mutation resource in maize comprising 2050 independent M2 mutant families in the elite tropical maize inbred ML10. By phenotypic screening, we showed that this population is of comparable quality with other mutagenized populations in maize. To illustrate the usefulness of this population for gene discovery, we performed rapid mapping-by-sequencing to clone a fasciated-ear mutant and identify a causal promoter deletion in ZmCLE7 (CLE7). Our mapping procedure does not require crossing to an unrelated parent, thus is suitable for mapping subtle traits and ones affected by heterosis. This first EMS population in tropical maize is expected to be very useful for the maize research community. Also, the EMS mutagenesis and rapid mapping-by-sequencing pipeline described here illustrate the power of performing forward genetics in diverse maize germplasms of choice, which can lead to novel gene discovery due to divergent genetic backgrounds.

scholarly journals Negative Effect of Camu-Camu (Myrciaria dubia) Despite High Vitamin C Content on Iron Bioavailability, Using a Caco-2 Cell Model

2014 ◽  
Vol 64 (1) ◽  
pp. 45-48 ◽  
Author(s):  
Yael Nemirovsky ◽  
Nelly Zavaleta ◽  
Maria Villanueva ◽  
Seth Armah ◽  
Sixto Iman ◽  
...  
Keyword(s):  
Vitamin C ◽  
Cell Model ◽  
Iron Bioavailability ◽  
High Vitamin ◽  
Myrciaria Dubia ◽  
Negative Effect

The Use of Caco-2 Cells to Estimate Fe Absorption in Humans - a Critical Appraisal

2010 ◽  
Vol 80 (45) ◽  
pp. 307-313 ◽  
Author(s):  
Ann-Sofie Sandberg
Keyword(s):  
Iron Uptake ◽  
Iron Absorption ◽  
Cell Model ◽  
Iron Bioavailability ◽  
Dietary Factors ◽  
Cell Uptake ◽  
Epithelial Cell Line ◽  
Cellular Iron ◽  
Cellular Iron Uptake

The Caco-2 cell model is widely used to assess the bioaccessibility/availability of iron from foods and diets. Analysis of iron uptake in this human epithelial cell line is usually preceded by a two-step digestion to simulate the conditions in the stomach and small intestine. Moreover, culturing the cells on inserts permits the measurement of iron transport. The cellular iron uptake is determined by direct measurements using radioisotopes, or indirectly by measurement of ferritin, the intracellular storage form of iron. There is a good correlation between Caco-2 cell uptake and human iron bioavailability for a number of dietary factors known to affect iron absorption. However, recent data suggest that in some cases there is no correlation. Possible reasons for such discrepancies, the benefits, and limitations of the Caco-2 cell model are discussed. In conclusion, in vitro experiments with Caco-2 cells are important tools for ranking foods with respect to bioavailability, for mechanistic studies of iron absorption, and for studies of dietary factors influencing absorption. The results need to be confirmed in humans.

scholarly journals Sodium pyrophosphate enhances iron bioavailability from bouillon cubes fortified with ferric pyrophosphate

2016 ◽  
Vol 116 (3) ◽  
pp. 496-503 ◽  
Author(s):  
Colin I. Cercamondi ◽  
Guus S. M. J. E. Duchateau ◽  
Rajwinder K. Harika ◽  
Robin van den Berg ◽  
Peter Murray ◽  
...  
Keyword(s):  
Cell Model ◽  
Ferrous Sulphate ◽  
Human Study ◽  
Iron Bioavailability ◽  
Sodium Pyrophosphate ◽  
Ferric Pyrophosphate ◽  
Simulated Digestion ◽  
Promising Strategy ◽  
Fe Isotopes

AbstractFe fortification of centrally manufactured and frequently consumed condiments such as bouillon cubes could help prevent Fe deficiency in developing countries. However, Fe compounds that do not cause sensory changes in the fortified product, such as ferric pyrophosphate (FePP), exhibit low absorption in humans. Tetra sodium pyrophosphate (NaPP) can form soluble complexes with Fe, which could increase Fe bioavailability. Therefore, the aim of this study was to investigate Fe bioavailability from bouillon cubes fortified with either FePP only, FePP+NaPP, ferrous sulphate (FeSO4) only, or FeSO4+NaPP. We first conducted in vitro studies using a protocol of simulated digestion to assess the dialysable and ionic Fe, and the cellular ferritin response in a Caco-2 cell model. Second, Fe absorption from bouillon prepared from intrinsically labelled cubes (2·5 mg stable Fe isotopes/cube) was assessed in twenty-four Fe-deficient women, by measuring Fe incorporation into erythrocytes 2 weeks after consumption. Fe bioavailability in humans increased by 46 % (P<0·005) when comparing bouillons fortified with FePP only (4·4 %) and bouillons fortified with FePP+NaPP (6·4 %). Fe absorption from bouillons fortified with FeSO4 only and with FeSO4+NaPP was 33·8 and 27·8 %, respectively (NS). The outcome from the human study is in agreement with the dialysable Fe from the in vitro experiments. Our findings suggest that the addition of NaPP could be a promising strategy to increase Fe absorption from FePP-fortified bouillon cubes, and if confirmed by further research, for other fortified foods with complex food matrices as well.

Kaempferol in Red and Pinto Bean Seed (Phaseolus vulgarisL.) Coats Inhibits Iron Bioavailability Using an in Vitro Digestion/Human Caco-2 Cell Model

2006 ◽  
Vol 54 (24) ◽  
pp. 9254-9261 ◽  
Author(s):  
Ying Hu ◽  
Zhiqiang Cheng ◽  
Larry I. Heller ◽  
Stuart B. Krasnoff ◽  
Raymond P. Glahn ◽  
...  
Keyword(s):  
Cell Model ◽  
In Vitro Digestion ◽  
Iron Bioavailability ◽  
Bean Seed ◽  
Pinto Bean

Assessment of nitrogen uptake and utilization in drought tolerant andStrigaresistant tropical maize varieties

2013 ◽  
Vol 60 (2) ◽  
pp. 195-207 ◽  
Author(s):  
Alpha Y. Kamara ◽  
Sylvester U. Ewansiha ◽  
Abebe Menkir
Keyword(s):  
Nitrogen Uptake ◽  
Tropical Maize ◽  
Drought Tolerant ◽  
Maize Varieties
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