A rapid and simple screening method ofCronobacterspp. in cell suspension and tofu

2012 ◽  
Vol 93 (6) ◽  
pp. 1520-1524 ◽  
Author(s):  
Sun Ae Kim ◽  
Ji Hyun Yu ◽  
Min Suk Rhee
PEDIATRICS ◽  
1959 ◽  
Vol 23 (4) ◽  
pp. 731-737
Author(s):  
Louis Gluck

A simple patch test, designed as a screening test for cystic fibrosis of the pancreas, is reported in preliminary form. In trials carried out with 207 patients it has proved accurate and reliable. A brief resume of the physiology of sweating, especially as pertinent to diagnostic methods in pediatrics, is also presented. Studies are in progress aiming toward further refinement of this method.


Author(s):  
Carlos Fernando Odir Rodrigues Melo ◽  
Luiz Claudio Navarro ◽  
Diogo Noin de Oliveira ◽  
Tatiane Melina Guerreiro ◽  
Estela de Oliveira Lima ◽  
...  

2000 ◽  
Vol 46 (4) ◽  
pp. 325-332 ◽  
Author(s):  
Sanae Kato ◽  
Isao Yumoto

To examine the distribution of the Na+-translocating NADH-quinone reductase (Na+-NQR) among marine bacteria, we developed a simple screening method for the detection of this enzyme. By reference to the homologous sequences of the Na+-NQR operons from Vibrio alginolyticus and Haemophilus influenzae, a pair of primers was designed for amplification of a part of the sixth ORF (nqr6) of the Na+-NQR operon. When PCR was performed using genomic DNA from 13 marine bacteria, a 0.9-kbp fragment corresponding to nqr6 was amplified in 10 strains. Although there were three PCR-negative strains phylogenetically, based on the sequence of the 16S rRNA, these were placed far from the PCR-positive strains. No product was observed in the case of nonmarine bacteria. The nucleotide and predicted amino acid sequences of nqr6 were highly conserved among the PCR-positive marine bacteria. A phylogenetic analysis of marine bacteria, based on nqr6 sequencing, was performed.Key words: Na+-translocating, NADH-quinone reductase, marine bacteria, PCR.


1980 ◽  
Vol 28 (9) ◽  
pp. 2779-2782 ◽  
Author(s):  
SEIICHI INAYAMA ◽  
YUKIKO TOKUNAGA ◽  
EIKICHI HOSOYA ◽  
TERUO NAKADATE ◽  
TETSUKICHI NIWAGUCHI ◽  
...  

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