Occurrence of Keto, Hydroxy and Epoxy Fatty Acids in Argemone mexicana Seed Oil

Seed-specific expression in Arabidopsis thaliana of oleate hydroxylase enzymes from castor bean and Lesquerella fendleri resulted in the accumulation of hydroxy fatty acids in the seed oil. By using various Arabidopsis mutant lines it was shown that the endoplasmic reticulum (ER) n–-3 desaturase (FAD3) and the FAE1 condensing enzyme are involved in the synthesis of polyunsaturated and very-long-chain hydroxy fatty acids, respectively. In Arabidopsis plants with an active ER Δ12-oleate desaturase the presence of hydroxy fatty acids corresponded to an increase in the levels of 18:1 and a decrease in 18:2 levels. Expression in yeast indicates that the castor hydroxylase also has a low level of desaturase activity.

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Dietary epoxy fatty acids are absorbed in healthy women

European Journal of Clinical Investigation ◽

10.1046/j.1365-2362.2002.00951.x ◽

2002 ◽

Vol 32 (2) ◽

pp. 79-83 ◽

Cited By ~ 26

Author(s):

R. Wilson ◽

C. E. Fernie ◽

C. M. Scrimgeour ◽

K. Lyall ◽

L. Smyth ◽

...

Keyword(s):

Fatty Acids ◽

Healthy Women ◽

Epoxy Fatty Acids

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Optimization of the Ratio of ω-6 Linoleic and ω-3 α-Linolenic Fatty Acids of Hemp Seed Oil with Jackknife and Bootstrap Resampling

Chemical Product and Process Modeling ◽

10.1515/cppm-2019-0028 ◽

2019 ◽

Vol 15 (1) ◽

Cited By ~ 1

Author(s):

Vibha Devi ◽

Shabina Khanam

Keyword(s):

Fatty Acids ◽

Standard Deviation ◽

Flow Rate ◽

Seed Oil ◽

Cannabis Sativa ◽

Bootstrap Resampling ◽

Hemp Seed ◽

Solvent Flow Rate ◽

The Mean ◽

Experimental Runs

Abstract In the present work, supercritical fluid extraction (SFE) of hemp (Cannabis sativa) seed oil at various ranges of SFE parameters is performed. These parameters and respective ranges are temperature (40–80) °C, pressure (200–350) bar, solvent (CO2) flow rate (5–15) g/min, particle size (0.43–1.02) mm and amount of co-solvent (ethanol) (0–10) % of solvent flow rate. Central composite design (CCD) suggests 32 experimental runs to perform through SFE. The obtained oil is analysed through gas chromatography to identify its fatty acids concentrations. The ratio of ω-6 linoleic and ω-3 α-linolenic fatty acids (ω-6/ω-3) is optimized through CCD to obtain the desired amount of 3:1 as this ratio is highly preferred for various health benefits. Ratio of ω-6/ω-3 is obtained in the range from 2.11 to 3.06:1 for all experimental runs. The effect of SFE parameters on this ratio is investigated. Further, cross-validation is peformed on the experimental data obtained for the concentrations of both fatty acids by jackknife and bootstrap resampling to authenticate the obtained data. Small value of standard deviation (~1), less standard error of the mean (SEM) (<0.8) and less variance coefficient (<0.11) confirms the validity of the obtained data. All the estimators’ values such as standard deviation, variance coefficients and SEM are observed in 95 % of confidence intervals.

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Evaluation of wound healing potential ofBhallatakadi Ghrita– cow ghee based polyherbal formulation:in-vivoexcision and incision wound model

Journal of Complementary and Integrative Medicine ◽

10.1515/jcim-2020-0179 ◽

2021 ◽

Vol 0 (0) ◽

Author(s):

Sandesh R. Wayal ◽

Shailendra S. Gurav

Keyword(s):

Fatty Acids ◽

Wound Healing ◽

Gallic Acid ◽

Quantitative Estimation ◽

Clinical Problem ◽

Treated Group ◽

Late Complications ◽

Argemone Mexicana ◽

Semecarpus Anacardium ◽

Western Ghats Of India

AbstractObjectivesGheeis widely considered as the Indian name for clarified butterfat and processing ofgheewith therapeutic herbs i.e.,ghritais renowned for augmenting their medicinal properties. The wound is considered as a challenging clinical problem with early and late complications. To reduce the burden of wounds with the shortest period and minimum scaring, an attempt was made to prepare and evaluate the wound healing potential ofgheebased polyherbal formulation.MethodsBased on local ethnic tribal claims,Semecarpus anacardiumL.,Argemone mexicanaL.,Cocculus hirsutusL., andWoodfordia fruticosaK. were collected from Western Ghats of India. The polyherbalBhallatakadi Ghrita(BG) formulation was prepared as perAyurvedicprocedure and assessed for its wound healing potential using incision and excision wound animal models.ResultsBG treated group showed a complete contraction of wounds (99.82 ± 0.10%) (p<0.001) with 15.17 ± 0.40 days re-epithelization time and breaking strength (531.50 ± 5.89) (p<0.05). The hydroxyproline content of BG was found to be significantly higher i.e., 4.23 ± 0.21 (p<0.05). Quantitative estimation of BG exhibited 54.7 ± 3.7 mg100 g−1of polyphenols and 42.3 ± 5.4 mg.100 g−1flavonoids in terms of gallic acid and quercetin respectively. HPLC analysis revealed the presence of gallic acid and quercetin whereas the presence of fatty acids was confirmed by GC-MS analysis.ConclusionsIt may conclude that the presence of quercetin, gallic acid, and fatty acids could have accelerated the healing rate of theghritaformulation, as they have already been known for their potential wound healing properties.

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