Temporal declines in Wadden Sea phytoplankton cell volumes observed within and across species

To comprehend structural changes in cardiac myocytes accompanying adrenergic innervation, it is essential that a three dimensional analysis be performed. To date, biological studies which utilize stereological methods have been limited to cells in tissue and in organs. Our laboratory has utilized current stereological techniques for measuring absolute volumes of individual myocytes in primary culture. Cell volumes are calculated for two distinct groups of cells at 96 hours in culture: isolated myocytes and myocytes innervated with adrenergic neurons (Figure 1).Cardiac myocytes are cultured from the ventricular apices of newborn rats. Cells are plated directly onto tissue culture dishes with or without preplated explants from the paravertebral thoracolumbar sympathetic chain. On day four cultures are photographed and marked for one-to-one cell location. Following conventional fixation and embeddment in eponate-12, the cells are relocated and mounted for microtomy. The cells are completely sectioned at 120nm in their parallel orientation to the surface of the dish (Figure 2). Serial sections are collected on formvar coated slotted grids and are recorded in sequence.

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La perception sociale des derniers endiguements de la Mer des Wadden : résultats d'un sondage d'opinion réalisé en Frise du Nord (Social perception of the latest dyking up on the Wadden Sea coast : results of an opinion poll in Northern Friesland)

Bulletin de l Association de géographes français ◽

10.3406/bagf.1995.1862 ◽

1995 ◽

Vol 72 (5) ◽

pp. 466-481 ◽

Cited By ~ 2

Author(s):

Lydie Goeldner

Keyword(s):

Social Perception ◽

Wadden Sea ◽

Opinion Poll ◽

Sea Coast

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Determinants of Erythrocyte Size in Chronic Liver Disease

Blood ◽

10.1182/blood.v34.6.739.739 ◽

1969 ◽

Vol 34 (6) ◽

pp. 739-746 ◽

Cited By ~ 10

Author(s):

THOMAS M. KILBRIDGE ◽

PAUL HELLER

Keyword(s):

Bone Marrow ◽

Liver Disease ◽

Chronic Liver Disease ◽

Peripheral Blood ◽

Hepatic Cirrhosis ◽

Alcoholic Cirrhosis ◽

Clinical Findings ◽

Red Cells ◽

Red Cell ◽

Cell Volumes

Abstract Serial determinations of red cell volumes were made with an electronic sizing device in 30 patients with hepatic cirrhosis. Variations in red cell volumes were correlated with other hematologic and clinical findings. The results of these studies suggest that volume macrocytosis in patients with alcoholic cirrhosis is either due to megaloblastosis of the bone marrow or to an accelerated influx of young red cells into the peripheral blood.

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The Wadden Sea: Denmark, The Netherlands, and Germany

Coastal World Heritage Sites - Coastal Research Library ◽

10.1007/978-94-024-1528-5_76 ◽

2018 ◽

pp. 521-526

Author(s):

Vanda Claudino-Sales

Keyword(s):

The Netherlands ◽

Wadden Sea

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4 Protecting the natural and cultural values of the Wadden Sea coast in the Anthropocene

Waddenland Outstanding ◽

10.1515/9789048537884-005 ◽

2018 ◽

pp. 67-80

Keyword(s):

Cultural Values ◽

Wadden Sea ◽

Sea Coast

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Phylogeography in an “oyster” shell provides first insights into the genetic structure of an extinct Ostrea edulis population

Scientific Reports ◽

10.1038/s41598-021-82020-x ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Sarah Hayer ◽

Dirk Brandis ◽

Alexander Immel ◽

Julian Susat ◽

Montserrat Torres-Oliva ◽

...

Keyword(s):

Large Scale ◽

Wadden Sea ◽

Atlantic Coast ◽

Phylogenetic Reconstruction ◽

Oyster Shell ◽

Glacial Refugia ◽

Late Nineteenth Century ◽

Ostrea Edulis ◽

Geographic Ranges ◽

The North

AbstractThe historical phylogeography of Ostrea edulis was successfully depicted in its native range for the first time using ancient DNA methods on dry shells from museum collections. This research reconstructed the historical population structure of the European flat oyster across Europe in the 1870s—including the now extinct population in the Wadden Sea. In total, four haplogroups were identified with one haplogroup having a patchy distribution from the North Sea to the Atlantic coast of France. This irregular distribution could be the result of translocations. The other three haplogroups are restricted to narrow geographic ranges, which may indicate adaptation to local environmental conditions or geographical barriers to gene flow. The phylogenetic reconstruction of the four haplogroups suggests the signatures of glacial refugia and postglacial expansion. The comparison with present-day O. edulis populations revealed a temporally stable population genetic pattern over the past 150 years despite large-scale translocations. This historical phylogeographic reconstruction was able to discover an autochthonous population in the German and Danish Wadden Sea in the late nineteenth century, where O. edulis is extinct today. The genetic distinctiveness of a now-extinct population hints at a connection between the genetic background of O. edulis in the Wadden Sea and for its absence until today.

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The Wadden Sea as a National Park and UNESCO World Heritage Site: Students’ Word Associations with These Two Conservation Designations

Sustainability ◽

10.3390/su13148006 ◽

2021 ◽

Vol 13 (14) ◽

pp. 8006

Author(s):

Till Schmäing ◽

Norbert Grotjohann

Keyword(s):

National Parks ◽

National Park ◽

Wadden Sea ◽

World Heritage ◽

World Heritage Site ◽

Unesco World Heritage ◽

Word Associations ◽

Heritage Site ◽

Unesco World Heritage Site ◽

Significant Difference

The Wadden Sea ecosystem is unique in many respects from a biological perspective. This is one reason why it is protected by national parks in Germany and by its designation as a UNESCO World Heritage Site. In biology didactics, there are only a few studies that focus on the Wadden Sea. This work investigates students’ word associations with the two stimulus words “national park” and “UNESCO World Heritage Site”. The survey was conducted among students living directly at the Wadden Sea and among students from the inland. The analysis of the identified associations (n = 8345) was carried out within the framework of a quantitative content analysis to be able to present and discuss the results on a group level. A statistically significant difference was found between the two groups. Overall, results showed that the students made subject-related associations as well as a large number of associations to both stimulus words that could be judged as non-subject-related. In some cases, a connection with the region of residence could be found, but this was not generally the case. Even students’ immediate residential proximity to the Wadden Sea is no guarantee that they have knowledge of the two considered protection terms.

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A Classification Scheme for Sediments and Habitats on Exposed Intertidal Flats with Multi-Frequency Polarimetric SAR

Remote Sensing ◽

10.3390/rs13030360 ◽

2021 ◽

Vol 13 (3) ◽

pp. 360

Author(s):

Wensheng Wang ◽

Martin Gade ◽

Kerstin Stelzer ◽

Jörn Kohlus ◽

Xinyu Zhao ◽

...

Keyword(s):

Classification Scheme ◽

Wadden Sea ◽

Relative Difference ◽

Polarimetric Synthetic Aperture Radar ◽

Intertidal Flats ◽

Sediment Types ◽

X Band ◽

Good Potential ◽

L Band ◽

Sar Data

We developed an extension of a previously proposed classification scheme that is based upon Freeman–Durden and Cloude–Pottier decompositions of polarimetric Synthetic Aperture Radar (SAR) data, along with a Double-Bounce Eigenvalue Relative Difference (DERD) parameter, and a Random Forest (RF) classifier. The extension was done, firstly, by using dual-copolarization SAR data acquired at shorter wavelengths (C- and X-band, in addition to the previously used L-band) and, secondly, by adding indicators derived from the (polarimetric) Kennaugh elements. The performance of the newly developed classification scheme, herein abbreviated as FCDK-RF, was tested using SAR data of exposed intertidal flats. We demonstrate that the FCDK-RF scheme is capable of distinguishing between different sediment types, namely mud and sand, at high spatial accuracies. Moreover, the classification scheme shows good potential in the detection of bivalve beds on the exposed flats. Our results show that the developed FCDK-RF scheme can be applied for the mapping of sediments and habitats in the Wadden Sea on the German North Sea coast using multi-frequency and multi-polarization SAR from ALOS-2 (L-band), Radarsat-2 (C-band) and TerraSAR-X (X-band).

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