Fully Reduced HMGB1‐Containing Peptide‐Based Polyurethane Scaffold with Minimal Functional Unit of Skin (MFUS) Enhances Large and Deep Wounded Skin Healing

2021 ◽  
pp. 2100403
Author(s):  
Peng Chang ◽  
Shijie Li ◽  
Qian Sun ◽  
Kai Guo ◽  
Heran Wang ◽  
...  
2019 ◽  
Vol 56 (3) ◽  
pp. 652-656
Author(s):  
Raul Chioibas ◽  
Florin Borcan ◽  
Ovidiu Mederle ◽  
Dana Stoian ◽  
Codruta Marinela Soica

Zinc oxide (ZnO) is an inorganic compound used for its antiseptic and skin healing properties. It is an excellent protective filter against UV radiation and it can be used as white pigment in pharmaceutical preparations. In this study, nano-ZnO particles were obtained by ultrasound treatment, and respectively by repeated freezing/heating process. The influence of synthesis method and of ultrasound generator parameters on the particles size and stability was observed. The results reveal that were obtained samples with a very good stability and sizes between 15 and 96 nm. It was found that synthesis based on ultrasound treatment lead to the formation of nanoparticles with lower sizes.


Function ◽  
2021 ◽  
Author(s):  
Bruce R Stevens ◽  
J Clive Ellory ◽  
Robert L Preston

Abstract The SARS-CoV-2 receptor, Angiotensin Converting Enzyme-2 (ACE2), is expressed at levels of greatest magnitude in the small intestine as compared to all other human tissues. Enterocyte ACE2 is co-expressed as the apical membrane trafficking partner obligatory for expression and activity of the B0AT1 sodium-dependent neutral amino acid transporter. These components are assembled as an [ACE2: B0AT1]2 dimer-of-heterodimers quaternary complex that putatively steers SARS-CoV-2 tropism in the gastrointestinal (GI) tract. GI clinical symptomology is reported in about half of COVID-19 patients, and can be accompanied by gut shedding of virion particles. We hypothesized that within this 4-mer structural complex, each [ACE2: B0AT1] heterodimer pair constitutes a physiological “functional unit.” This was confirmed experimentally by employing purified lyophilized enterocyte brush border membrane vesicles that were exposed to increasing doses of high-energy electron radiation from a 16 MeV linear accelerator. Based on established target theory, the results indicated the presence of Na+-dependent neutral amino acid influx transport activity functional unit with target size mw = 183.7 ± 16.8 kDa in situ in intact apical membranes. Each thermodynamically stabilized [ACE2: B0AT1] heterodimer functional unit manifests the transport activity within the whole ∼345 kDa [ACE2: B0AT1]2 dimer-of-heterodimers quaternary structural complex. The results are consistent with our prior molecular docking modeling and gut-lung axis approaches to understanding COVID-19. These findings advance the understanding of the physiology of B0AT1 interaction with ACE2 in the gut, and thereby potentially contribute to translational developments designed to treat or mitigate COVID-19 variant outbreaks and/or GI symptom persistence in long-haul Post-Acute Sequelae of SARS-CoV-2 (PASC).


Genetics ◽  
2000 ◽  
Vol 154 (3) ◽  
pp. 1115-1123 ◽  
Author(s):  
John R Halsall ◽  
Michael J Milner ◽  
Lorna A Casselton

Abstract The B mating type locus of the basidiomycete Coprinus cinereus encodes a large family of lipopeptide pheromones and their seven transmembrane domain receptors. Here we show that the B42 locus, like the previously described B6 locus, derives its unique specificity from nine multiallelic genes that are organized into three subgroups each comprising a receptor and two pheromone genes. We show that the three genes within each group are kept together as a functional unit by being embedded in an allele-specific DNA sequence. Using a combination of sequence analysis, Southern blotting, and DNA-mediated transformation with cloned genes, we demonstrate that different B loci may share alleles of one or two groups of genes. This is consistent with the prediction that the three subgroups of genes are functionally redundant and that it is the different combinations of their alleles that generate the multiple B mating specificities found in nature. The B42 locus was found to contain an additional gene, mfs1, that encodes a putative multidrug transporter belonging to the major facilitator family. In strains with other B mating specificities, this gene, whose functional significance was not established, lies in a region of shared homology flanking the B locus.


Author(s):  
Margret Weissbach ◽  
Marius Neugebauer ◽  
Anna-Christin Joel

AbstractSpider silk attracts researchers from the most diverse fields, such as material science or medicine. However, still little is known about silk aside from its molecular structure and material strength. Spiders produce many different silks and even join several silk types to one functional unit. In cribellate spiders, a complex multi-fibre system with up to six different silks affects the adherence to the prey. The assembly of these cribellate capture threads influences the mechanical properties as each fibre type absorbs forces specifically. For the interplay of fibres, spinnerets have to move spatially and come into contact with each other at specific points in time. However, spinneret kinematics are not well described though highly sophisticated movements are performed which are in no way inferior to the movements of other flexible appendages. We describe here the kinematics for the spinnerets involved in the cribellate spinning process of the grey house spider, Badumna longinqua, as an example of spinneret kinematics in general. With this information, we set a basis for understanding spinneret kinematics in other spinning processes of spiders and additionally provide inspiration for biomimetic multiple fibre spinning.


1928 ◽  
Vol 11 (5) ◽  
pp. 547-562 ◽  
Author(s):  
Ernst Wolf

The frequency of contraction of the bell of Gonionemus was studied in relation to temperature, with intact animals and also where different operations were made on the nervous system. A number of values of µ are found for intact animals namely 8,100±, 10,500±, 32,000± and 22,500±, with critical temperatures at 9.6°, 12.3°, and 14.0°. Four different classes of operations were used: (1) Animals where the nerve ring was cut on two opposite sides of the bell; the µ values found are 10,500± and 21,300±, with a critical temperature at 13.4°. (2) Animals with four cuts through the nerve ring gave µ = 10,600 ± and µ = 21,000, with a critical temperature at 13.1°. (3) In animals where the bell was cut in half the temperature characteristic was found to be 16,900. And finally (4) in the animals where the nerve ring was totally removed µ values of 8,100, 16,000±, and 29,000 were found, with critical temperatures at 15.0° and 9.4°. These results are discussed from the standpoint of the theory which supposes that definite "temperature characteristics" may be associated with the functional activity of particular elements in a complex functional unit, and that these elements may be separately studied and identified by suitable experimental procedures involving the magnitudes of the respective temperature characteristics and the locations of associated critical temperatures. The swimming bell of medusæ with its marginal sense organs permits a fairly direct approach to such questions. It is found that even slight injuries to the marginal nerve ring, for example, produce specific modifications in the temperature relations which are different from those appearing when the organism is cut in half.


2018 ◽  
Vol 26 ◽  
pp. 332-343 ◽  
Author(s):  
Weiwen Jiang ◽  
Edwin H.-M. Sha ◽  
Xianzhang Chen ◽  
Lin Wu ◽  
Qingfeng Zhuge

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