Sarcolipin knockout mice fed a high-fat diet exhibit altered indices of adipose tissue inflammation and remodeling

Obesity ◽  
2016 ◽  
Vol 24 (7) ◽  
pp. 1499-1505 ◽  
Author(s):  
Rebecca E.K. MacPherson ◽  
Daniel Gamu ◽  
Scott Frendo-Cumbo ◽  
Laura Castellani ◽  
Frenk Kwon ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Knockout Mice ◽  
High Fat Diet ◽  
Adipose Tissue Inflammation ◽  
High Fat ◽  
Tissue Inflammation

scholarly journals Spred2 Regulates High Fat Diet-Induced Adipose Tissue Inflammation, and Metabolic Abnormalities in Mice

2019 ◽  
Vol 10 ◽  
Author(s):  
Takahiro Ohkura ◽  
Teizo Yoshimura ◽  
Masayoshi Fujisawa ◽  
Toshiaki Ohara ◽  
Rie Marutani ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
High Fat Diet ◽  
Metabolic Abnormalities ◽  
Adipose Tissue Inflammation ◽  
High Fat ◽  
Tissue Inflammation

scholarly journals Luteolin Improves Insulin Resistance in Postmenopausal Obese Mice by Altering Macrophage Polarization (FS12-01-19)

2019 ◽  
Vol 3 (Supplement_1) ◽  
Author(s):  
Yunjung Baek ◽  
Mi Nam Lee ◽  
Dayong Wu ◽  
Munkyong Pae
Keyword(s):  
Insulin Resistance ◽  
Body Weight ◽  
Adipose Tissue ◽  
High Fat Diet ◽  
Ovarian Function ◽  
Body Weight Gain ◽  
Adipose Tissue Inflammation ◽  
High Fat ◽  
Tissue Inflammation ◽  
Ovariectomized Mice

Abstract Objectives Previously, we showed that loss of ovarian function in mice fed high-fat diet exacerbated insulin resistance and adipose tissue inflammation. In the current study, we tested whether consumption of luteolin, an anti-inflammatory flavonoid, could mitigate adipose tissue inflammation and insulin resistance in obese ovariectomized mice. Methods Nine-week-old ovariectomized C57BL/6 mice were fed a low-fat diet (LFD), high-fat diet (HFD), or HFD supplemented with 0.005% luteolin (HFD + L) for 16 weeks. The anti-inflammatory drug salicylate was used as a positive control. Fasting blood glucose, insulin, and insulin resistance index HOMA-IR were measured every 4 weeks. Adipose tissue and spleen were characterized for tissue inflammation by real-time PCR and immune cell populations by flow cytometry after 16 weeks of feeding. Results HFD resulted in more body weight gain than LFD in ovariectomized mice and supplementing HFD with 0.005% luteolin did not affect the body weight gain. In addition, HFD elicited a significant elevation in fat mass, which were comparable between HFD and HFD + L groups. However, luteolin supplementation resulted in a significant decrease in CD11c+ macrophages in gonadal adipose tissue, as well as a trend of decrease in macrophage infiltration. Luteolin supplementation also significantly decreased mRNA expression of inflammatory and M1 markers MCP-1, CD11c, TNF-a, and IL-6, while maintaining expression of M2 marker MGL1. We further found that luteolin treatment protected mice from insulin resistance induced by HFD consumption; this improved insulin resistance was correlated with reductions in CD11c+ adipose tissue macrophages. Conclusions Our findings indicate that dietary luteolin supplementation attenuates adipose tissue inflammation and insulin resistance found in mice with loss of ovarian function coupled with a HFD intake, and this effect may be partly mediated through suppressing M1-like polarization of macrophages in adipose tissue. These results have clinical implication in implementing dietary intervention for prevention of metabolic syndrome associated with postmenopause and obesity. Funding Sources Supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIP) (NRF-2018R1A1A1A05078886).

scholarly journals Lack of Interleukin-1 Receptor I (IL-1RI) Protects Mice From High-Fat Diet-Induced Adipose Tissue Inflammation Coincident With Improved Glucose Homeostasis

Diabetes ◽  
2011 ◽  
Vol 60 (6) ◽  
pp. 1688-1698 ◽  
Author(s):  
F. C. McGillicuddy ◽  
K. A. Harford ◽  
C. M. Reynolds ◽  
E. Oliver ◽  
M. Claessens ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Glucose Homeostasis ◽  
High Fat Diet ◽  
Interleukin 1 ◽  
Adipose Tissue Inflammation ◽  
High Fat ◽  
Tissue Inflammation

scholarly journals Blockade of VEGF-C and VEGF-D modulates adipose tissue inflammation and improves metabolic parameters under high-fat diet

2015 ◽  
Vol 4 (2) ◽  
pp. 93-105 ◽  
Author(s):  
Sinem Karaman ◽  
Maija Hollmén ◽  
Marius R. Robciuc ◽  
Annamari Alitalo ◽  
Harri Nurmi ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
High Fat Diet ◽  
Metabolic Parameters ◽  
Adipose Tissue Inflammation ◽  
High Fat ◽  
Tissue Inflammation

The Early Effects of High Fat Diet and Exercise on Markers of Adipose Tissue Inflammation in Mice

2011 ◽  
Vol 43 (Suppl 1) ◽  
pp. 685-686
Author(s):  
Yair Pincu ◽  
Melissa A. Linden ◽  
Stephen A. Martin ◽  
Jeffrey A. Woods ◽  
Tracy Baynard
Keyword(s):  
Adipose Tissue ◽  
High Fat Diet ◽  
Adipose Tissue Inflammation ◽  
High Fat ◽  
Tissue Inflammation ◽  
Diet And Exercise ◽  
Early Effects

scholarly journals The Absence of Adiponectin Alters Niacin’s Effects on Adipose Tissue Inflammation in Mice

Nutrients ◽  
2020 ◽  
Vol 12 (8) ◽  
pp. 2427
Author(s):  
Emily C. Graff ◽  
Han Fang ◽  
Desiree Wanders ◽  
Robert L. Judd
Keyword(s):  
Adipose Tissue ◽  
High Fat Diet ◽  
Inflammatory Markers ◽  
Adipose Tissue Inflammation ◽  
High Fat ◽  
Tissue Inflammation ◽  
Low Fat Diet ◽  
Expression Of Genes ◽  
Ccl2 Expression ◽  
Anti Inflammatory

Obesity is an immunometabolic disease associated with chronic inflammation and the dysregulation of pro- and anti-inflammatory cytokines. One hallmark of obesity is reduced concentrations of the anti-inflammatory adipokine, adiponectin. Pharmacologic doses of niacin produce multiple metabolic benefits, including attenuating high-fat diet (HFD)-induced adipose tissue inflammation and increasing adiponectin concentrations. To determine if adiponectin mediates the anti-inflammatory effects of niacin, male C57BL/6J (WT) and adiponectin null (Adipoq-/-) mice were maintained on a low-fat diet (LFD) or HFD for 6 weeks, before being administered either vehicle or niacin (360 mg/kg/day) for 5 weeks. HFD-fed mice had increased expression of genes associated with macrophage recruitment (Ccl2) and number (Cd68), and increased crown-like structure (CLS) number in adipose tissue. While niacin attenuated Ccl2 expression, there were no effects on Cd68 or CLS number. The absence of adiponectin did not hinder the ability of niacin to reduce Ccl2 expression. HFD feeding increased gene expression of inflammatory markers in the adipose tissue of WT and Adipoq-/- mice. While niacin tended to decrease the expression of inflammatory markers in WT mice, niacin increased their expression in HFD-fed Adipoq-/- mice. Therefore, our results indicate that the absence of adiponectin alters the effects of niacin on markers of adipose tissue inflammation in HFD-fed mice, suggesting that the effects of niacin on tissue cytokines may involve adiponectin.

scholarly journals Chia Seed (Salvia hispanica L.) Digested Total Protein Prevented Adipose Tissue Inflammation and Reduce Obesity Complications in Mice Fed a High-Fat Diet

2020 ◽  
Vol 4 (Supplement_2) ◽  
pp. 436-436
Author(s):  
Hercia Martino ◽  
Mariana Grancieri ◽  
Renata Toledo ◽  
Thaisa Veridiano ◽  
Cintia Tomaz Sant'Ana ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Waist Circumference ◽  
Total Cholesterol ◽  
High Fat Diet ◽  
Negative Control ◽  
Standard Diet ◽  
Adipose Tissue Inflammation ◽  
High Fat ◽  
Tissue Inflammation ◽  
Chia Seed

Abstract Objectives To evaluate the effects of digested total proteins (DTP) from chia seed to prevent adipogenesis and adipose tissue inflammation in mice fed a high-fat diet. Methods C57Bl/6 black mice (n = 44; 8 weeks old) where divided in 4 groups (n = 12 each): negative control (NC; standard diet AIN-93M); positive control (PC; high fat diet- HFD- 60% of lipids); normal diet + DTP (NH; standard diet + 400 mg/kg of body/day of DTP); high-fat diet plus DTP (HFH; HFD + 400 mg/kg of body/day of DTP). After 9 weeks of treatment, the animals were euthanized and the blood and the adipose tissue (total) were collected. Plasma was used to analyze total cholesterol, high-density (HDL) and low-density (LDL) lipoprotein cholesterol, triacylglycerides (TGL), aspartate (AST) and alanine (ALT) aminotransferase levels by colorimetry. Waist circumference was measured by metric tape in the middle portion between the anus and mouth and the quantity of p-p65-NF-κB and PPAR-Y ELISA test. Histomorphometric analysis was determined in adipose tissue staining with hematoxylin/eosin to determined adipocytes area and foci of inflammation by the average of 1000 cells/group. Data were analyzed by ANOVA and post-hoc of Newman-Kews (P < 0.05). The study protocol was approved by the Ethics Committee of the Federal University of Viçosa (Protocol 01/2019). Results DTP from chia seed reduced the plasmatic levels of total cholesterol (−17.5%), LDL (−42.8%), TGL (−12.3%), and waist circumference (−5.5%) in obesity mice DTP-treated (P < 0.05). The treatment with DTP reduced the adipocytes area in HFH group by −15.1% and the foci of inflammation in −78.1% in comparison with PC (P < 0.05). The levels of p-p65-NF-κB in adipose tissue were reduced by DTP in mice fed a HFD in −41.1% (P < 0.05). However, PPAR-γ levels, body fat (%), Lee index, and HDL levels were not changed by DTP (P > 0.05). The levels of AST and ALT were not affected by HFD or DTP (P > 0.05). Conclusions DTP from chia seed had an anti-inflammatory and even an anti-adipogenic effect. These results show the effectiveness of digested proteins from chia seed against obesity and its associated inflammation. Funding Sources CNPq and CAPES (Brazil), and ACES (USA).

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