Mutations at sterol 14α‐demethylases ( CYP51A &B) confer the DMI resistance in Colletotrichum gloeosporioides from grape

2020 ◽  
Vol 76 (12) ◽  
pp. 4093-4103
Author(s):  
Jin Wang ◽  
Dongya Shi ◽  
Lingling Wei ◽  
Wenchan Chen ◽  
Weiwei Ma ◽  
...  
Keyword(s):  
Colletotrichum Gloeosporioides ◽  
Dmi Resistance

scholarly journals Mutations and Overexpression of CYP51 Associated with DMI-Resistance in Colletotrichum gloeosporioides from Chili

Plant Disease ◽  
2020 ◽  
Vol 104 (3) ◽  
pp. 668-676 ◽  
Author(s):  
Ling-ling Wei ◽  
Wen-chan Chen ◽  
Wei-cheng Zhao ◽  
Jin Wang ◽  
Bing-ran Wang ◽  
...  
Keyword(s):  
Binding Site ◽  
Colletotrichum Gloeosporioides ◽  
Resistance Mechanism ◽  
Resistance Management ◽  
Amino Acid Sequences ◽  
Cross Resistance ◽  
Single Spore ◽  
Genotype I ◽  
Genotype Iii ◽  
Dmi Resistance

Chili anthracnose caused by Colletotrichum spp. is an annual production concern for growers in China. Sterol C14-demethylation inhibitors (DMIs, such as tebuconazole) have been widely used to control this disease for more than three decades. In the current study, of 48 isolates collected from commercial chili farms in Jiangsu Province of China during 2018 and 2019, 8 single-spore isolates were identified as Colletotrichum gloeosporioides and the rest were identified as C. acutatum. To determine whether the DMI resistance of isolates develops in the field, mycelial growth of the 48 isolates was measured in culture medium with and without tebuconazole. In all, 6 of the 8 C. gloeosporioides isolates were resistant to tebuconazole, but all 40 of the C. acutatum isolates were sensitive to tebuconazole. The fitness cost of resistance was low based on a comparison of fitness parameters between the sensitive and resistant isolates of C. gloeosporioides. Positive cross-resistance was observed between tebuconazole and difenconazole or propiconazole, but not prochloraz. Alignment results of the CgCYP51 amino acid sequences from the sensitive and resistant isolates indicated that mutations can be divided into three genotypes. Genotype I possessed four substitutions (V18F, L58V, S175P, and P341A) at the CgCYP51A gene but no substitutions at CgCYP51B, while genotype II had five substitutions (L58V, S175P, A340S, T379A, and N476T) at CgCYP51A, concomitant with three substitutions (D121N, T132A, and F391Y) at CgCYP51B. In addition, genotype III contained two substitutions (L58V and S175P) at CgCYP51A, concomitant with one substitution (T262A) at CgCYP51B. Molecular docking models illustrated that the affinity of tebuconazole to the binding site of the CgCYP51 protein from the resistant isolates was decreased when compared with binding site affinity of the sensitive isolates. Our findings provide not only novel insights into understanding the resistance mechanism to DMIs, but also some important references for resistance management of C. gloeosporioides on chili.

Colletotrichum Gloeosporioides Species Complex: Pathogen Causing Anthracnose, Gummosis and Die-Back Diseases of Cashew (Anacardium Occidentale L.) In Ghana

2020 ◽  
Vol 2 (6) ◽  
Author(s):  
A. Muntala ◽  
P. M. Norshie ◽  
K. G. Santo ◽  
C. K. S. Saba
Keyword(s):  
Species Complex ◽  
Colletotrichum Gloeosporioides ◽  
Potato Dextrose Agar ◽  
Causal Agent ◽  
Anacardium Occidentale ◽  
Causative Organism ◽  
Pathogenicity Test ◽  
Small Water ◽  
Colletotrichum Gloeosporioides Species Complex

A survey was conducted in twenty-five cashew (Anacardium occidentale) orchards in five communities in the Dormaa-Central Municipality of Bono Region of Ghana to assess the incidence and severity of anthracnose, gummosis and die-back diseases on cashew. Cashew diseased samples of leaves, stem, inflorescences, twigs, flowers, nuts and apples showing symptoms (e. g. small, water-soaked, circular or irregular yellow, dark or brown spots or lesions on leaves, fruits and flowers, sunken surface, especially on the apples, blight, gum exudates) were collected for isolation of presumptive causative organism. The pathogen was isolated after disinfecting the excised diseased pieces in 70% ethanol, plated on potato dextrose agar (PDA) and incubated at 28 oC for 3 to 7 days. The identity of the putative pathogen was morphologically and culturally confirmed as belonging to Colletotrichum gloeosporioides species complex using standard mycological identification protocols. The pathogen had varied conidia sizes of between 9-15 up to 20 μm in length and diameter of 3-6 μm. The conidia were straight and cylindrically shaped with rounded or obtuse ends. The septate mycelium was whitish-grey, velvety and cotton-like in appearance from the top. The results confirmed the presence of the pathogen in the orchards with incidence ranging from 6.9% and 14.0% for gummosis and averaged 22.9% for anthracnose infected orchards. The result of the pathogenicity test confirmed the isolates to be pathogenic on inoculated cashew seedlings and were consistently re-isolated, thereby establishing the pathogen as the true causal agent of the said diseases in cashew trees and thus completed the Koch’s postulate.

METODOLOGIA DE INOCULAÇÃO DE Colletotrichum gloeosporioides EM FRUTOS DE MAMÃO

2012 ◽  
Vol 24 (3) ◽  
pp. 183-188
Author(s):  
Lahyre Izaete Silveira Gomes ◽  
Eduardo Alves ◽  
Enilton Nascimento de Santana ◽  
Hilário Antônio de Castro
Keyword(s):  
Colletotrichum Gloeosporioides

Control of Winged Waterprimrose (Jussiaea decurrens) and Northern Jointvetch (Aeschynomene virginica) with Fungal Pathogens

Weed Science ◽  
1979 ◽  
Vol 27 (5) ◽  
pp. 497-501 ◽  
Author(s):  
C. D. Boyette ◽  
G. E. Templeton ◽  
R. J. Smith
Keyword(s):  
Oryza Sativa ◽  
Glycine Max ◽  
Fungal Pathogens ◽  
Liquid Culture ◽  
Field Experiments ◽  
Colletotrichum Gloeosporioides ◽  
Pathogenic Fungus ◽  
Field Tests ◽  
Wide Range ◽  
Growing Region

An indigenous, host-specific, pathogenic fungus that parasitizes winged waterprimrose [Jussiaea decurrens(Walt.) DC.] is endemic in the rice growing region of Arkansas. The fungus was isolated and identified asColletotrichum gloeosporioides(Penz.) Sacc. f.sp. jussiaeae(CGJ). It is highly specific for parasitism of winged waterprimrose and not parasitic on creeping waterprimrose (J. repensL. var.glabrescensKtze.), rice (Oryza sativaL.), soybeans [Glycine max(L.) Merr.], cotton (Gossypium hirsutumL.), or 4 other crops and 13 other weeds. The fungus was physiologically distinct from C.gloeosporioides(Penz.) Sacc. f. sp.aeschynomene(CGA), an endemic anthracnose pathogen of northern jointvetch[Aeschynomene virginica(L.) B.S.P.], as indicated by cross inoculations of both weeds. Culture in the laboratory and inoculation of winged waterprimrose in greenhouse, growth chamber and field experiments indicated that the pathogen was stable, specific, and virulent in a wide range of environments. The pathogen yielded large quantities of spores in liquid culture. It is suitable for control of winged waterprimrose. Winged waterprimrose and northern jointvetch were controlled in greenhouse and field tests by application of spore mixtures of CGJ and CGA at concentrations of 1 to 2 million spores/ml of each fungus in 94 L/ha of water; the fungi did not damage rice or nontarget crops.

Molecular and physiological characterization of Arabidopsis‐Colletotrichum gloeosporioides pathosystem

2021 ◽  
Author(s):  
Mengze Gao ◽  
Miting Wan ◽  
Liyun Yang ◽  
Meng Zhao ◽  
Xiaojin Liu ◽  
...  
Keyword(s):  
Colletotrichum Gloeosporioides ◽  
Physiological Characterization

scholarly journals Publisher Correction: Bioactivities of Allium longicuspis Regel against anthracnose of mango caused by Colletotrichum gloeosporioides (Penz.)

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Dionisio de Guzman Alvindia ◽  
Mark Anthony Angeles Mangoba
Keyword(s):  
Colletotrichum Gloeosporioides ◽  
Allium Longicuspis

An amendment to this paper has been published and can be accessed via a link at the top of the paper.

Inherent tolerance of Colletotrichum gloeosporioides to fludioxonil

2020 ◽  
pp. 104767
Author(s):  
Guido Schnabel ◽  
Qin Tan ◽  
Verena Schneider ◽  
Hideo Ishii
Keyword(s):  
Colletotrichum Gloeosporioides

scholarly journals Antifungal Activity of Propyl Disulfide from Neem (Azadirachta indica) in Vapor and Agar Diffusion Assays against Anthracnose Pathogens (Colletotrichum gloeosporioides and Colletotrichum acutatum) in Mango Fruit

2021 ◽  
Vol 9 (4) ◽  
pp. 839
Author(s):  
Muhammad Rafiullah Khan ◽  
Vanee Chonhenchob ◽  
Chongxing Huang ◽  
Panitee Suwanamornlert
Keyword(s):  
Antifungal Activity ◽  
Mycelial Growth ◽  
Colletotrichum Gloeosporioides ◽  
Colletotrichum Acutatum ◽  
Model Parameters ◽  
Pathogenicity Test ◽  
Agar Diffusion ◽  
Wide Range ◽  
Significant Difference ◽  
Diffusion Assay

Microorganisms causing anthracnose diseases have a medium to a high level of resistance to the existing fungicides. This study aimed to investigate neem plant extract (propyl disulfide, PD) as an alternative to the current fungicides against mango’s anthracnose. Microorganisms were isolated from decayed mango and identified as Colletotrichum gloeosporioides and Colletotrichum acutatum. Next, a pathogenicity test was conducted and after fulfilling Koch’s postulates, fungi were reisolated from these symptomatic fruits and we thus obtained pure cultures. Then, different concentrations of PD were used against these fungi in vapor and agar diffusion assays. Ethanol and distilled water were served as control treatments. PD significantly (p ≤ 0.05) inhibited more of the mycelial growth of these fungi than both controls. The antifungal activity of PD increased with increasing concentrations. The vapor diffusion assay was more effective in inhibiting the mycelial growth of these fungi than the agar diffusion assay. A good fit (R2, 0.950) of the experimental data in the Gompertz growth model and a significant difference in the model parameters, i.e., lag phase (λ), stationary phase (A) and mycelial growth rate, further showed the antifungal efficacy of PD. Therefore, PD could be the best antimicrobial compound against a wide range of microorganisms.

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