High-performance thin-layer chromatography plate blotting for liquid microjunction surface sampling probe mass spectrometric analysis of analytes separated on a wettable phase plate

2011 ◽  
Vol 26 (1) ◽  
pp. 37-42 ◽  
Author(s):  
Matthew J. Walworth ◽  
Joseph J. Stankovich ◽  
Gary J. Van Berkel ◽  
Michael Schulz ◽  
Susanne Minarik
Keyword(s):  
High Performance ◽  
Thin Layer Chromatography Plate ◽  
Mass Spectrometric ◽  
Spectrometric Analysis ◽  
Phase Plate ◽  
Surface Sampling ◽  
Sampling Probe ◽  
Chromatography Plate ◽  
Surface Sampling Probe ◽  
Layer Chromatography

scholarly journals Planar Chromatography for the Multiclass, Multiresidue Screening of Chloramphenicol, Nitrofuran, and Sulfonamide Residues in Pork and Beef

1997 ◽  
Vol 80 (4) ◽  
pp. 737-740 ◽  
Author(s):  
Jean-Pierre Abjean
Keyword(s):  
High Performance ◽  
Thin Layer Chromatography Plate ◽  
Amino Derivative ◽  
Residue Level ◽  
Long Wave ◽  
Qualitative Detection ◽  
Animal Muscle ◽  
Chromatography Plate ◽  
Sulfonamide Residues ◽  
Layer Chromatography

Abstract A method is described for multiclass and multiresidue qualitative detection of chloramphenicol, nitrofuran, and sulfonamide residues in animal muscle. The drugs are extracted from 1 g tissue with 2 mL ethyl acetate and purified by silica solidphase extraction. After elution of the cartridge, the collected solution is evaporated, and the residue is dissolved in methanol and chromatographed on a Si60 high-performance thin-layer chromatography plate. After evaporation of solvent, nitrofurans are visualized first by their specific UV photochemical reaction with pyridine. Then chloramphenicol is reduced to its amino derivative, and this derivative and the sulfonamides are visualized by long-wave UV after reaction with fluorescamine. Chloramphenicol, nitrofurans, and sulfonamides are detected at residue level of 10, 5, and 100 μg/kg, respectively, or less in pork and beef.

Thin-Layer Chromatography and Electrospray Mass Spectrometry Coupled Using a Surface Sampling Probe

2002 ◽  
Vol 74 (24) ◽  
pp. 6216-6223 ◽  
Author(s):  
Gary J. Van Berkel ◽  
Amaury D. Sanchez ◽  
J. Martin E. Quirke
Keyword(s):  
Mass Spectrometry ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Electrospray Mass Spectrometry ◽  
Surface Sampling ◽  
Sampling Probe ◽  
Surface Sampling Probe ◽  
Layer Chromatography

scholarly journals HPTLC Fingerprint Profile and Identification of Antidiabetic and Antioxidant Leads from Bauhinia rufescens L

2020 ◽  
Vol 2020 ◽  
pp. 1-18
Author(s):  
Wadah Osman ◽  
Mona S Mohammed ◽  
Hassan S Khalid ◽  
Abdelkhalig Muddathir ◽  
Shaza W. Shantier ◽  
...  
Keyword(s):  
High Performance ◽  
Mass Spectrometric ◽  
Spectrometric Analysis ◽  
Antioxidant Compounds ◽  
Major Health ◽  
Treatment Of Diabetes ◽  
Layer Chromatography

Diabetes is one of the world’s major health problems, and many reports have supported the role of oxidative stress in the pathogenesis of both type 1 and type 2 diabetes. The present study aims to evaluate the antidiabetic and antioxidant activity of Bauhinia rufescens, a plant used in Sudanese folkloric medicine for the treatment of diabetes. It was also aimed to identify isolates and characterize the bioactive antidiabetic and antioxidant compounds using bioactivity-guided fractionation followed by high-performance thin-layer chromatography (HPTLC) autobiography, liquid chromatography-mass spectrometric analysis, and nuclear magnetic resonance (NMR). Two potential compounds were successfully isolated and identified which may provide new leads for more potent analogues in drug discovery.

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