Metabolic Study of Ginsenoside Rg3 and Glimepiride in Type 2 Diabetic Rats by Liquid Chromatography Coupled with Quadrupole‐Orbitrap Mass Spectrometry

2021 ◽  
Author(s):  
Di Yang ◽  
Xue Li ◽  
Yunhua Fu ◽  
Xingyu Tao ◽  
Fei Zheng ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Diabetic Rats ◽  
Ginsenoside Rg3 ◽  
Metabolic Study ◽  
Orbitrap Mass Spectrometry ◽  
Type 2 Diabetic

scholarly journals Ethanol extract of mulberry leaves partially restores the composition of intestinal microbiota and strengthens liver glycogen fragility in type 2 diabetic rats

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Zhan-Zhong Liu ◽  
Qing-Hua Liu ◽  
Zhao Liu ◽  
Jia-Wei Tang ◽  
Eng-Guan Chua ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Intestinal Microbiota ◽  
Ethanol Extract ◽  
Liver Glycogen ◽  
Diabetic Rats ◽  
Size Exclusion ◽  
Mulberry Leaves ◽  
Positive Effects ◽  
Type 2 Diabetic

Abstract Background Mulberry leaf as a traditional Chinese medicine is able to treat obesity, diabetes, and dyslipidemia. It is well known that diabetes leads to intestinal microbiota dysbiosis. It is also recently discovered that liver glycogen structure is impaired in diabetic animals. Since mulberry leaves are able to improve the diabetic conditions through reducing blood glucose level, it would be interesting to investigate whether they have any positive effects on intestinal microbiota and liver glycogen structure. Methods In this study, we first determined the bioactive components of ethanol extract of mulberry leaves via high-performance liquid chromatography (HPLC) and liquid chromatography/mass spectrometry (LC/MS). Murine animal models were divided into three groups, normal Sprague-Dawley (SD) rats, high-fat diet (HFD) and streptozotocin (STZ) induced type 2 diabetic rats, and HFD/STZ-induced rats administered with ethanol extract of mulberry leaves (200 mg/kg/day). Composition of intestinal microbiota was analyzed via metagenomics by sequencing the V3-V4 region of 16S rDNAs. Liver glycogen structure was characterized through size exclusion chromatography (SEC). Both Student’s t-test and Tukey’s test were used for statistical analysis. Results A group of type 2 diabetic rat models were successfully established. Intestinal microbiota analysis showed that ethanol extract of mulberry leaves could partially change intestinal microbiota back to normal conditions. In addition, liver glycogen was restored from fragile state to stable state through administration of ethanol extract of mulberry leaves. Conclusions This study confirms that the ethanol extract of mulberry leaves (MLE) ameliorates intestinal microbiota dysbiosis and strengthens liver glycogen fragility in diabetic rats. These finding can be helpful in discovering the novel therapeutic targets with the help of further investigations.

Fecal Metabolomics of Type 2 Diabetic Rats and Treatment with Gardenia jasminoides Ellis Based on Mass Spectrometry Technique

2018 ◽  
Vol 66 (6) ◽  
pp. 1591-1599 ◽  
Author(s):  
Yuan Zhou ◽  
Lihui Men ◽  
Zifeng Pi ◽  
Mengying Wei ◽  
Fengrui Song ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Diabetic Rats ◽  
Gardenia Jasminoides ◽  
Mass Spectrometry Technique ◽  
Spectrometry Technique ◽  
Gardenia Jasminoides Ellis ◽  
Type 2 Diabetic
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