scholarly journals Mesenchymal stromal cells pretreated with pro‐inflammatory cytokines promote skin wound healing through VEGFC ‐mediated angiogenesis

2020 ◽  
Vol 9 (10) ◽  
pp. 1218-1232 ◽  
Author(s):  
Mengting Zhu ◽  
Yunpeng Chu ◽  
Qianwen Shang ◽  
Zhiyuan Zheng ◽  
Yanan Li ◽  
...  
Keyword(s):  
Wound Healing ◽  
Inflammatory Cytokines ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Skin Wound ◽  
Skin Wound Healing ◽  
Pro Inflammatory Cytokines

scholarly journals Mesenchymal stromal cells-exosomes: a promising cell-free therapeutic tool for wound healing and cutaneous regeneration

2019 ◽  
Vol 7 ◽  
Author(s):  
Peng Hu ◽  
Qinxin Yang ◽  
Qi Wang ◽  
Chenshuo Shi ◽  
Dali Wang ◽  
...  
Keyword(s):  
Wound Healing ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Messenger Rna ◽  
Cell Types ◽  
Skin Wound ◽  
Effector Proteins ◽  
Skin Wound Healing ◽  
New Research

Abstact Cutaneous regeneration at the wound site involves several intricate and dynamic processes which require a series of coordinated interactions implicating various cell types, growth factors, extracellular matrix (ECM), nerves, and blood vessels. Mesenchymal stromal cells (MSCs) take part in all the skin wound healing stages playing active and beneficial roles in animal models and humans. Exosomes, which are among the key products MSCs release, mimic the effects of parental MSCs. They can shuttle various effector proteins, messenger RNA (mRNA) and microRNAs (miRNAs) to modulate the activity of recipient cells, playing important roles in wound healing. Moreover, using exosomes avoids many risks associated with cell transplantation. Therefore, as a novel type of cell-free therapy, MSC-exosome -mediated administration may be safer and more efficient than whole cell. In this review, we provide a comprehensive understanding of the latest studies and observations on the role of MSC-exosome therapy in wound healing and cutaneous regeneration. In addition, we address the hypothesis of MSCs microenvironment extracellular vesicles (MSCs-MEVs) or MSCs microenvironment exosomes (MSCs-MExos) that need to take stock of and solved urgently in the related research about MSC-exosomes therapeutic applications. This review can inspire investigators to explore new research directions of MSC-exosome therapy in cutaneous repair and regeneration.

Modulated mesenchymal stromal cells improve skin wound healing

Biologicals ◽  
2020 ◽  
Vol 67 ◽  
pp. 1-8
Author(s):  
Ausra Liubaviciute ◽  
Tatjana Ivaskiene ◽  
Gene Biziuleviciene
Keyword(s):  
Wound Healing ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Skin Wound ◽  
Skin Wound Healing

In vitro comparative study of human mesenchymal stromal cells from dermis and adipose tissue for application in skin wound healing

2019 ◽  
Vol 13 (5) ◽  
pp. 729-741 ◽  
Author(s):  
Helena Debiazi Zomer ◽  
Gisele Kristina dos Santos Varela ◽  
Priscilla Barros Delben ◽  
Diana Heck ◽  
Talita da Silva Jeremias ◽  
...  
Keyword(s):  
Wound Healing ◽  
Adipose Tissue ◽  
Comparative Study ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Skin Wound ◽  
Skin Wound Healing ◽  
Human Mesenchymal Stromal Cells

Bioimaging assessment and effect of skin wound healing using bone-marrow-derived mesenchymal stromal cells with the artificial dermis in diabetic rats

2008 ◽  
Vol 13 (6) ◽  
pp. 064036 ◽  
Author(s):  
Hirokazu Inoue ◽  
Takashi Murakami ◽  
Takashi Ajiki ◽  
Mayumi Hara ◽  
Yuichi Hoshino ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Wound Healing ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Skin Wound ◽  
Diabetic Rats ◽  
Skin Wound Healing ◽  
Artificial Dermis

scholarly journals Mesenchymal Stromal Cells and Cutaneous Wound Healing: A Comprehensive Review of the Background, Role, and Therapeutic Potential

2018 ◽  
Vol 2018 ◽  
pp. 1-13 ◽  
Author(s):  
Michael S. Hu ◽  
Mimi R. Borrelli ◽  
H. Peter Lorenz ◽  
Michael T. Longaker ◽  
Derrick C. Wan
Keyword(s):  
Wound Healing ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Wound Repair ◽  
Therapeutic Potential ◽  
Skin Wound ◽  
Skin Wound Healing ◽  
Differentiation Potential ◽  
Cutaneous Wound ◽  
Aging Populations

Cutaneous wound repair is a highly coordinated cascade of cellular responses to injury which restores the epidermal integrity and its barrier functions. Even under optimal healing conditions, normal wound repair of adult human skin is imperfect and delayed healing and scarring are frequent occurrences. Dysregulated wound healing is a major concern for global healthcare, and, given the rise in diabetic and aging populations, this medicoeconomic disease burden will continue to rise. Therapies to reliably improve nonhealing wounds and reduce scarring are currently unavailable. Mesenchymal stromal cells (MSCs) have emerged as a powerful technique to improve skin wound healing. Their differentiation potential, ease of harvest, low immunogenicity, and integral role in native wound healing physiology make MSCs an attractive therapeutic remedy. MSCs promote cell migration, angiogenesis, epithelialization, and granulation tissue formation, which result in accelerated wound closure. MSCs encourage a regenerative, rather than fibrotic, wound healing microenvironment. Recent translational research efforts using modern bioengineering approaches have made progress in creating novel techniques for stromal cell delivery into healing wounds. This paper discusses experimental applications of various stromal cells to promote wound healing and discusses the novel methods used to increase MSC delivery and efficacy.

O014 Critical role of Th17 pro-inflammatory cytokines to delay skin wound healing

Cytokine ◽  
2012 ◽  
Vol 59 (3) ◽  
pp. 503 ◽  
Author(s):  
I. Paris ◽  
S. Charreau ◽  
E. Guignouard ◽  
M. Garnier ◽  
L. Favot-Laforge ◽  
...  
Keyword(s):  
Wound Healing ◽  
Inflammatory Cytokines ◽  
Critical Role ◽  
Skin Wound ◽  
Skin Wound Healing ◽  
Pro Inflammatory Cytokines

Lipidomics of Mesenchymal Stromal Cells: Understanding the Adaptation of Phospholipid Profile in Response to Pro-Inflammatory Cytokines

2015 ◽  
Vol 231 (5) ◽  
pp. 1024-1032 ◽  
Author(s):  
Ana Margarida Campos ◽  
Elisabete Maciel ◽  
Ana S. P. Moreira ◽  
Bebiana Sousa ◽  
Tânia Melo ◽  
...  
Keyword(s):  
Inflammatory Cytokines ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Phospholipid Profile ◽  
Pro Inflammatory Cytokines

scholarly journals Extracellular Vesicles from Adipose-Derived Mesenchymal Stem/Stromal Cells Accelerate Migration and Activate AKT Pathway in Human Keratinocytes and Fibroblasts Independently of miR-205 Activity

2017 ◽  
Vol 2017 ◽  
pp. 1-14 ◽  
Author(s):  
Andrea da Fonseca Ferreira ◽  
Pricila da Silva Cunha ◽  
Virgínia Mendes Carregal ◽  
Priscila de Cássia da Silva ◽  
Marcelo Coutinho de Miranda ◽  
...  
Keyword(s):  
Wound Healing ◽  
Extracellular Vesicles ◽  
Stromal Cells ◽  
Average Diameter ◽  
Skin Wound ◽  
Akt Pathway ◽  
Skin Wound Healing ◽  
Akt Phosphorylation ◽  
Akt Activation

Mesenchymal stem/stromal cells (MSCs) are promising tools in cell therapy. They secrete extracellular vesicles (EVs) that carry different classes of molecules that can promote skin repair, but the mechanisms are poorly understood. Skin wound healing is a complex process that requires the activity of several signaling pathways and cell types, including keratinocytes and fibroblasts. In this study, we explored whether adipose tissue MSC-derived EVs could accelerate migration and proliferation of keratinocytes and fibroblasts, activate the AKT pathway, and promote wound healing in vivo. Furthermore, we evaluated if EV effects are miR-205 dependent. We found that MSC EVs had an average diameter of 135 nm. Keratinocytes and fibroblasts exposed to EVs exhibited higher levels of proliferation, migration, and AKT activation. Topical administration of EVs accelerated skin wound closure. Knockdown of miR-205 decreased AKT phosphorylation in fibroblasts and keratinocytes, whereas migration was decreased only in keratinocytes. Moreover, knockdown of miR-205 failed to inhibit AKT phosphorylation in fibroblasts and keratinocytes exposed to EVs. About the mechanism of EV effects, we found that incubation with EVs prevented inhibition of AKT activation by miR-205 knockdown, suggesting that EVs activate AKT independently of miR-205. In conclusion, we demonstrated that EVs are a promising tool for wound healing.

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