Scaffold 3D‐Printed from Metallic Nanoparticles‐Containing Ink Simultaneously Eradicates Tumor and Repairs Tumor‐Associated Bone Defects

BACKGROUND: An increasing number of bone graft materials are commercially available and vary in their composition, mechanism of action, costs, and indications. OBJECTIVE: A commercially available PLGA scaffold produced using 3D printing technology has been used to promote the preservation of the alveolar socket after tooth extraction. We examined its influence on bone regeneration in long bones of New Zealand White rabbits. METHODS: 5.0-mm-diameter circular defects were created on the tibia bones of eight rabbits. Two groups were studied: (1) control group, in which the bone defects were left empty; (2) scaffold group, in which the PLGA scaffolds were implanted into the bone defect. Radiography was performed every two weeks postoperatively. After sacrifice, bone specimens were isolated and examined by micro-computed tomography and histology. RESULTS: Scaffolds were not degraded by eight weeks after surgery. Micro-computed tomography and histology showed that in the region of bone defects that was occupied by scaffolds, bone regeneration was compromised and the total bone volume/total volume ratio (BV/TV) was significantly lower. CONCLUSION: The implantation of this scaffold impedes bone regeneration in a non-critical bone defect. Implantation of bone scaffolds, if unnecessary, lead to a slower rate of fracture healing.

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Evaluation of a poly(lactic-acid) scaffold filled with poly(lactide-co-glycolide)/hydroxyapatite nanofibres for reconstruction of a segmental bone defect in a canine model

Veterinární Medicína ◽

10.17221/80/2019-vetmed ◽

2019 ◽

Vol 64 (No. 12) ◽

pp. 531-538

Author(s):

JW Yun ◽

SY Heo ◽

MH Lee ◽

HB Lee

Keyword(s):

Lactic Acid ◽

Bone Defect ◽

Bone Defects ◽

Bone Morphogenetic Protein 2 ◽

Poly Lactic Acid ◽

Micro Computed Tomography ◽

Bone Parameters ◽

3D Printed ◽

Group 3 ◽

Group 2

Critical-sized bone defects are a difficult problem in both human and veterinary medicine. To address this issue, synthetic graft materials have been garnering attention. Abundant in vitro studies have proven the possibilities of poly(lactic-acid) (PLA) scaffolds and poly(lactide-co-glycolide)/hydroxyapatite (PLGA/HAp) nanofibres for treating bone defects. The present study aimed at conducting an in vivo assessment of the biological performance of a three dimensional (3D)-printed PLA scaffold filled with a PLGA/HAp nanofibrous scaffold to estimate its potential applications in bone defect reconstruction surgery. Defects were created in a 20 mm-long region of the radius bone. The defects created on the right side in six Beagle dogs (n = 6) were left untreated (Group 1). The defects on the left side (n = 6) were filled with 3D-printed PLA scaffolds incorporated with PLGA/Hap nanofibres with gelatine (Group 2). The other six Beagle dog defects were made bilaterally (n = 12) and filled with the same material as that used in Group 2 along with recombinant bone morphogenetic protein 2 (rhBMP-2) (Group 3). Both the radiological and histological examinations were performed for observing the reaction of the scaffold and the bone. Micro-computed tomography (CT) was utilised for the evaluation of the bone parameters 20 weeks after the experiment. The radiological and histological results revealed that the scaffold was biodegradable and was replaced by new bone tissue. The micro-CT revealed that the bone parameters were significantly (P < 0.05) increased in Group 3. Based on these results, our study serves as a foundation for future studies on bone defect treatment using synthetic polymeric scaffolds.

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Fabrication and Characterization of Ta–GelMA–BG Scaffolds by Chemical Crosslinking Processing for Promotion Osteointegration

Frontiers in Materials ◽

10.3389/fmats.2021.701268 ◽

2021 ◽

Vol 8 ◽

Author(s):

Fujian Zhao ◽

Xiongfa Ji ◽

Yang Yan ◽

Zhen Yang ◽

Xiaofeng Chen ◽

...

Keyword(s):

Early Stage ◽

Bone Defects ◽

Chemical Crosslinking ◽

Composite Scaffolds ◽

Load Bearing ◽

3D Printed ◽

Fabrication And Characterization ◽

Cell Adhesion And Proliferation

The repair of bone defects in load-bearing positions still faces great challenges. Tantalum (Ta) has attempted to repair bone defects based on the excellent mechanical properties. However, the osseointegration of Ta needs to be improved due to the lack of osteoinduction. Herein, tantalum–gelatin–methacryloyl–bioactive glass (Ta–GelMA–BG) scaffolds were successfully fabricated by loading BG in 3D-printed Ta scaffolds through a chemical crosslinking method. The results showed that the composite scaffolds have the ability to promote cell adhesion and proliferation. The incorporation of BG resulted in a significant increase in apatite-forming and osteogenesis differentiation abilities. In vivo results indicated that the Ta–GelMA–BG scaffolds significantly enhanced the osteointegration at the early stage after implantation. Overall, the Ta–GelMA–BG scaffolds are a promising platform for the load bearing bone regeneration field.

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Repair of critical-size porcine craniofacial bone defects using a collagen-polycaprolactone composite biomaterial

10.1101/2021.04.19.440506 ◽

2021 ◽

Author(s):

Marley J Dewey ◽

Derek J Milner ◽

Daniel Weisgerber ◽

Colleen Flanagan ◽

Marcello Rubessa ◽

...

Keyword(s):

Bone Formation ◽

Bone Regeneration ◽

Fibrous Tissue ◽

Bone Defects ◽

Large Animal ◽

Collagen Scaffolds ◽

Shape Fitting ◽

Large Animal Models ◽

3D Printed ◽

Mineralized Collagen

Regenerative medicine approaches for massive craniomaxillofacial bone defects face challenges associated with the scale of missing bone, the need for rapid graft-defect integration, and challenges related to inflammation and infection. Mineralized collagen scaffolds have been shown to promote mesenchymal stem cell osteogenesis due to their porous nature and material properties, but are mechanically weak, limiting surgical practicality. Previously, these scaffolds were combined with 3D-printed polycaprolactone mesh to form a scaffold-mesh composite to increase strength and promote bone formation in sub-critical sized porcine ramus defects. Here, we compare the performance of mineralized collagen-polycaprolactone composites to the polycaprolactone mesh in a critical-sized porcine ramus defect model. While there were no differences in overall healing response between groups, our data demonstrated broadly variable metrics of healing regarding new bone infiltration and fibrous tissue formation. Abscesses were present surrounding some implants and polycaprolactone polymer was still present after 9-10 months of implantation. Overall, while there was limited successful healing, with 2 of 22 implants showed substantial levels of bone regeneration, and others demonstrating some form of new bone formation, the results suggest targeted improvements to improve repair of large animal models to more accurately represent craniomaxillofacial bone healing. Notably, strategies to increase osteogenesis throughout the implant, modulate the immune system to support repair, and employ shape-fitting tactics to avoid implant micromotion and resultant fibrosis. Improvements to the mineralized collagen scaffolds involve changes in pore size and shape to increase cell migration and osteogenesis and inclusion or delivery of factors to aid vascular ingrowth and bone regeneration.

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Three-dimensional printed porous tantalum prosthesis for treating inflammation after total knee arthroplasty in one-stage surgery – a case report

Journal of International Medical Research ◽

10.1177/0300060519891280 ◽

2019 ◽

Vol 48 (3) ◽

pp. 030006051989128

Author(s):

Fuyou Wang ◽

Hao Chen ◽

Pengfei Yang ◽

Aikeremujiang Muheremu ◽

Peng He ◽

...

Keyword(s):

Total Knee Arthroplasty ◽

Chronic Inflammation ◽

Knee Arthroplasty ◽

Three Dimensional ◽

Bone Defects ◽

Left Knee ◽

Severe Osteoporosis ◽

Porous Tantalum ◽

3D Printed ◽

Total Knee

Chronic inflammation and bone defects after total knee arthroplasty are a challenge for the orthopedic surgeon. There have been few reports on application of a three-dimensional (3D) printed porous tantalum prosthesis in such situations. We report an 83-year-old female patient who presented to our clinic with consistent pain of the left knee for 10 years and a severe decline in mobility for 2 years. Chronic inflammation, loosening of a tibial prosthesis with a bone defect, and severe osteoporosis were diagnosed. The patient was treated with computer designed and manufactured, personalized, 3D printed porous pure tantalum pad-assisted left total knee arthroplasty. The surgery went smoothly and the patient achieved a satisfactory recovery after surgery. A 3D printed porous tantalum prosthesis can be used to reconstruct tibial bone defects in patients with chronic inflammation after joint replacement surgeries.

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Light based synthesis of metallic nanoparticles on surface-modified 3D printed substrates for high performance electronic systems

Additive Manufacturing ◽

10.1016/j.addma.2020.101367 ◽

2020 ◽

Vol 34 ◽

pp. 101367

Author(s):

Reza N. Esfahani ◽

Matthew P. Shuttleworth ◽

Viktor Doychinov ◽

Nathan J. Wilkinson ◽

Jack Hinton ◽

...

Keyword(s):

Metallic Nanoparticles ◽

High Performance ◽

Electronic Systems ◽

3D Printed ◽

Surface Modified

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3D-printed device for synthesis of magnetic and metallic nanoparticles

Journal of Flow Chemistry ◽

10.1007/s41981-020-00124-3 ◽

2020 ◽

Author(s):

Vini Singh

Keyword(s):

Metallic Nanoparticles ◽

3D Printed

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Mesoporous bioactive glass-coated 3D printed borosilicate bioactive glass scaffolds for improving repair of bone defects

International Journal of Biological Sciences ◽

10.7150/ijbs.23872 ◽

2018 ◽

Vol 14 (4) ◽

pp. 471-484 ◽

Cited By ~ 12

Author(s):

Xin Qi ◽

Hui Wang ◽

Yadong Zhang ◽

Libin Pang ◽

Wei Xiao ◽

...

Keyword(s):

Bioactive Glass ◽

Bone Defects ◽

3D Printed ◽

Mesoporous Bioactive Glass

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3D printed constructs with antibacterial or antitumor activity for surgical treatment of bone defects in cancer patients

10.1063/1.5001642 ◽

2017 ◽

Author(s):

N. S. Sergeeva ◽

I. K. Sviridova ◽

V. S. Komlev ◽

P. A. Karalkin ◽

V. A. Kirsanova ◽

...

Keyword(s):

Surgical Treatment ◽

Antitumor Activity ◽

Cancer Patients ◽

Bone Defects ◽

3D Printed

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Reconstruction for Massive Proximal Tibial Bone Defects Using Patient-customized Three-dimensional-printed Metaphyseal Cones in Revision Total Knee Arthroplasty

10.21203/rs.3.rs-84721/v1 ◽

2020 ◽

Author(s):

Yang Li ◽

Xinguang Wang ◽

Hua Tian

Keyword(s):

Total Knee Arthroplasty ◽

Knee Arthroplasty ◽

Three Dimensional ◽

Bone Defects ◽

Revision Total Knee Arthroplasty ◽

3D Printed ◽

Total Knee ◽

The Mean ◽

Highly Porous

Abstract Background: The reconstruction of massive bone defects is one of the main challenges in revision total knee arthroplasty (RTKA). Although several methods are available, each of them has its prominent shortcomings. The purpose of this study is to review the clinical outcomes of RTKA with massive proximal tibial bone defects using patient-customized three-dimensional (3D)-printed highly porous metaphyseal cones.Methods: We retrospectively reviewed seven RTKAs with Anderson Orthopaedic Research Institute (AORI) type III tibial defects using patient-customized 3D-printed highly porous metaphyseal cones, which have been performed at a single institution between 2016 and 2018. Results: The mean age at diagnosis of the patients was 68 years old (61-77). The mean length of follow-up was 25.3 months (19-36). At the latest follow-up, no aseptic loosening or prosthetic joint infection has been determined. The mean HSS increased from 49 (39-63) to 78 (70-83) (P<0.01); the mean WOMAC increased from 59 (46-73) to 26 (12-38) (P<0.01). All patients obtained the range of motion and mechanical alignment improvement postoperatively.Conclusion: The patient-customized 3D-printed metaphyseal cone could be a promising technique in addressing severe tibial defects in RTKA. Our study shows encouraging short-term clinical and radiological outcomes with no aseptic loosening, periprosthetic infection, or fracture. Nevertheless, further follow-up and the expansion of sample size are needed to demonstrate the advantage of this innovative technique fully.

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