scholarly journals The ISWI ATPase Smarca5 (Snf2h) Is Required for Proliferation and Differentiation of Hematopoietic Stem and Progenitor Cells

Stem Cells ◽  
2017 ◽  
Vol 35 (6) ◽  
pp. 1614-1623 ◽  
Author(s):  
Juraj Kokavec ◽  
Tomas Zikmund ◽  
Filipp Savvulidi ◽  
Vojtech Kulvait ◽  
Winfried Edelmann ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Hematopoietic Stem ◽  
Proliferation And Differentiation ◽  
Stem And Progenitor Cells

scholarly journals Mechanism of Action of Diallyl Sulphide in Ameliorating the Hematopoietic Radiation Injury

2021 ◽  
Author(s):  
Omika Katoch ◽  
Mrinalini Tiwari ◽  
Namita Kalra ◽  
Paban K. Agrawala
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Progenitor Cells ◽  
Hematopoietic Stem ◽  
Proliferation And Differentiation ◽  
Stem And Progenitor Cells ◽  
Radiation Induced ◽  
Medicinal Properties ◽  
Marrow Cellularity

AbstractDiallyl sulphide (DAS), the pungent component of garlic, is known to have several medicinal properties and has recently been shown to have radiomitigative properties. The present study was performed to better understand its mode of action in rendering radiomitigation. Evaluation of the colonogenic ability of hematopoietic progenitor cells (HPCs) on methocult media, proliferation and differentiation of hematopoietic stem cells (HSCs), and transplantation of stem cells were performed. The supporting tissue of HSCs was also evaluated by examining the histology of bone marrow and in vitro colony-forming unit–fibroblast (CFU-F) count. Alterations in the levels of IL-5, IL-6 and COX-2 were studied as a function of radiation or DAS treatment. It was observed that an increase in proliferation and differentiation of hematopoietic stem and progenitor cells occurred by postirradiation DAS administration. It also resulted in increased circulating and bone marrow homing of transplanted stem cells. Enhancement in bone marrow cellularity, CFU-F count, and cytokine IL-5 level were also evident. All those actions of DAS that could possibly add to its radiomitigative potential and can be attributed to its HDAC inhibitory properties, as was observed by the reversal radiation induced increase in histone acetylation.

scholarly journals BLOS2 negatively regulates Notch signaling during neural and hematopoietic stem and progenitor cell development

eLife ◽  
2016 ◽  
Vol 5 ◽  
Author(s):  
Wenwen Zhou ◽  
Qiuping He ◽  
Chunxia Zhang ◽  
Xin He ◽  
Zongbin Cui ◽  
...  
Keyword(s):  
Notch Signaling ◽  
Progenitor Cells ◽  
Progenitor Cell ◽  
Neural Progenitor Cells ◽  
Hematopoietic Stem ◽  
Lysosomal Trafficking ◽  
Embryonic Lethal ◽  
Proliferation And Differentiation ◽  
Stem And Progenitor Cells ◽  
Lysosome Related Organelles

Notch signaling plays a crucial role in controling the proliferation and differentiation of stem and progenitor cells during embryogenesis or organogenesis, but its regulation is incompletely understood. BLOS2, encoded by the Bloc1s2 gene, is a shared subunit of two lysosomal trafficking complexes, biogenesis of lysosome-related organelles complex-1 (BLOC-1) and BLOC-1-related complex (BORC). Bloc1s2−/− mice were embryonic lethal and exhibited defects in cortical development and hematopoiesis. Loss of BLOS2 resulted in elevated Notch signaling, which consequently increased the proliferation of neural progenitor cells and inhibited neuronal differentiation in cortices. Likewise, ablation of bloc1s2 in zebrafish or mice led to increased hematopoietic stem and progenitor cell production in the aorta-gonad-mesonephros region. BLOS2 physically interacted with Notch1 in endo-lysosomal trafficking of Notch1. Our findings suggest that BLOS2 is a novel negative player in regulating Notch signaling through lysosomal trafficking to control multiple stem and progenitor cell homeostasis in vertebrates.

scholarly journals Hematopoietic Stem Cell Dynamics are Regulated by Progenitor Demand: Lessons from a Quantitative Modeling Approach

2018 ◽  
Author(s):  
Markus Klose ◽  
Maria Carolina Florian ◽  
Hartmut Geiger ◽  
Ingmar Glauche
Keyword(s):  
Progenitor Cells ◽  
Cell Dynamics ◽  
Hematopoietic Stem ◽  
Modeling Approach ◽  
Hematopoietic Recovery ◽  
Proliferation And Differentiation ◽  
Stem And Progenitor Cells ◽  
Quantitative Understanding ◽  
Varying Demand ◽  
Prevailing View

AbstractThe prevailing view on murine hematopoiesis and on hematopoietic stem cells (HSC) in particular derives from experiments that are related to regeneration after irradiation and HSC transplantation. However, over the past years, different experimental techniques have been developed to investigate hematopoiesis under homeostatic conditions, thereby providing access to proliferation and differentiation rates of hematopoietic stem and progenitor cells in the unperturbed situation. Moreover, it has become clear that hematopoiesis undergoes distinct changes during aging with large effects on HSC abundance, lineage contribution, asymmetry of division and self-renewal potential. However, it is currently not fully resolved how stem and progenitor cells interact to respond to varying demands and how this balance is altered by an aging-induced shift in HSC polarity.Here, we present anin-silicomodel to investigate the dynamics of HSC response to varying demand. By introducing an internal feedback between stem and progenitor cells, the model is suited to consistently describe both hematopoietic homeostasis and regeneration, including the limited regulation of HSCs in the homeostatic situation. The model further explains the age-dependent increase in phenotypic HSCs as a consequence of the cells’ inability to preserve divisional asymmetry.Our model suggests a dynamically regulated population of intrinsically asymmetrically dividing HSCs as suitable control mechanism that adheres with many qualitative and quantitative findings on hematopoietic recovery after stress and aging. The modeling approach thereby illustrates how a mathematical formalism can support the conceptual and the quantitative understanding of regulatory principles in HSC biology.

scholarly journals BCAP inhibits proliferation and differentiation of myeloid progenitors in the steady state and during demand situations

Blood ◽  
2017 ◽  
Vol 129 (11) ◽  
pp. 1503-1513 ◽  
Author(s):  
Jeffrey M. Duggan ◽  
Matthew B. Buechler ◽  
Rebecca M. Olson ◽  
Tobias M. Hohl ◽  
Jessica A. Hamerman
Keyword(s):  
Steady State ◽  
Progenitor Cells ◽  
Myeloid Cell ◽  
Cell Development ◽  
Hematopoietic Stem ◽  
Key Points ◽  
Proliferation And Differentiation ◽  
Stem And Progenitor Cells ◽  
A Cell ◽  
Myeloid Progenitors

Key Points BCAP is expressed in hematopoietic stem and progenitor cells and inhibits myeloid cell development in a cell-intrinsic manner. In the absence of BCAP, hematopoietic stem and progenitor cells are more proliferative, particularly in demand situations.

Flow cytometric enumeration and immunophenotyping of hematopoietic stem and progenitor cells

2001 ◽  
Vol 38 (2) ◽  
pp. 139-147
Author(s):  
Jan W. Gratama ◽  
D. Robert Sutherland ◽  
Michael Keeney
Keyword(s):  
Progenitor Cells ◽  
Hematopoietic Stem ◽  
Flow Cytometric ◽  
Stem And Progenitor Cells
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