Treating mouse skull defects with 3D‐printed fatty acid and tricalcium phosphate implants

3D printed biomaterials have been extensively investigated and developed in the field of bone regeneration related to clinical issues. However, specific applications of 3D printed biomaterials in different dental areas have seldom been reported. In this study, we aimed to and successfully fabricated 3D poly (lactic-co-glycolic acid)/β-tricalcium phosphate (3D-PLGA/TCP) and 3D β-tricalcium phosphate (3D-TCP) scaffolds using two relatively distinct 3D printing (3DP) technologies. Conjunctively, we compared and investigated mechanical and biological responses on human dental pulp stem cells (hDPSCs). Physicochemical properties of the scaffolds, including pore structure, chemical elements, and compression modulus, were characterized. hDPSCs were cultured on scaffolds for subsequent investigations of biocompatibility and osteoconductivity. Our findings indicate that 3D printed PLGA/TCP and β-tricalcium phosphate (β-TCP) scaffolds possessed a highly interconnected and porous structure. 3D-TCP scaffolds exhibited better compressive strength than 3D-PLGA/TCP scaffolds, while the 3D-PLGA/TCP scaffolds revealed a flexible mechanical performance. The introduction of 3D structure and β-TCP components increased the adhesion and proliferation of hDPSCs and promoted osteogenic differentiation. In conclusion, 3D-PLGA/TCP and 3D-TCP scaffolds, with the incorporation of hDPSCs as a personalized restoration approach, has a prospective potential to repair minor and critical bone defects in oral and maxillofacial surgery, respectively.

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Physical characterization and in vitro evaluation of 3D printed hydroxyapatite, tricalcium phosphate, zirconia, alumina, and SiAlON structures made by lithographic ceramic manufacturing

MRS Advances ◽

10.1557/adv.2020.229 ◽

2020 ◽

Vol 5 (46-47) ◽

pp. 2419-2428

Author(s):

Alexander K. Nguyen ◽

Peter L. Goering ◽

Shelby A. Skoog ◽

Roger J. Narayan

Keyword(s):

Tricalcium Phosphate ◽

Force Microscopy ◽

Atomic Force ◽

3D Printed ◽

Ceramic Manufacturing ◽

Surface Morphologies ◽

Combination Of Material ◽

Amorphous Character ◽

Cells Cultured

AbstractIn this study, lithographic ceramic manufacturing was used to create solid chips out of hydroxyapatite, tricalcium phosphate, zirconia, alumina, and SiAlON ceramic. X-ray powder diffraction of each material confirmed that the chips were crystalline, with little amorphous character that could result from remaining polymeric binder, and were composed entirely out of the ceramic feedstock. Surface morphologies and roughnesses were characterized using atomic force microscopy. Human bone marrow stem cells cultured with osteogenic supplements on each material type expressed alkaline phosphatase levels, an early marker of osteogenic differentiation, on par with cells cultured on a glass control. However, cells cultured on the tricalcium phosphate-containing material expressed lower levels of ALP suggesting that osteoinduction was impaired on this material. Further analyses should be conducted with these materials to identify underlying issues of the combination of material and analysis method.

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Implantation of green tea catechin α-tricalcium phosphate combination enhances bone repair in rat skull defects

Journal of Biomedical Materials Research Part B Applied Biomaterials ◽

10.1002/jbm.b.31848 ◽

2011 ◽

Vol 98B (2) ◽

pp. 263-271 ◽

Cited By ~ 23

Author(s):

Reena Rodriguez ◽

Hisamoto Kondo ◽

Myat Nyan ◽

Jia Hao ◽

Takayuki Miyahara ◽

...

Keyword(s):

Green Tea ◽

Tricalcium Phosphate ◽

Bone Repair ◽

Skull Defects ◽

Green Tea Catechin

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Enhanced In Vivo Bone and Blood Vessel Formation by Iron Oxide and Silica Doped 3D Printed Tricalcium Phosphate Scaffolds

Annals of Biomedical Engineering ◽

10.1007/s10439-018-2040-8 ◽

2018 ◽

Vol 46 (9) ◽

pp. 1241-1253 ◽

Cited By ~ 22

Author(s):

Susmita Bose ◽

Dishary Banerjee ◽

Samuel Robertson ◽

Sahar Vahabzadeh

Keyword(s):

Iron Oxide ◽

Blood Vessel ◽

Tricalcium Phosphate ◽

Blood Vessel Formation ◽

Vessel Formation ◽

3D Printed

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A Comparative Study of Granular Agglomeration between 3D Printed Hydroxyapatite and Commercial Bone Graft Granules

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.798.83 ◽

2019 ◽

Vol 798 ◽

pp. 83-87

Author(s):

Faungchat Thammarakcharoen ◽

Ariya Yampakdee ◽

Bovornwut Buranawat ◽

Jintamai Suwanprateeb

Keyword(s):

Phase Composition ◽

Comparative Study ◽

Bone Graft ◽

Water Absorption ◽

Tricalcium Phosphate ◽

Water Film ◽

Xrd Analysis ◽

High Porosity ◽

Capillary Action ◽

3D Printed

Granule characteristics and the agglomeration ability of 3D printed hydroxyapatite granules (3DP HA) when contacting water were compared to those of commercial bone graft granules based on hydroxyapatite/β-tricalcium phosphate/collagen mixture (Sunmax). Microstructure, phase composition, water absorption and granular agglomeration of the granules were characterized. SEM showed that the granule sizes of Sunmax were in the range of 0.8-1.5 mm whereas that of 3DP HA was relatively more uniform at about 1 mm. 3DP HA granules comprised the weaving of numerous minute crystals containing large pores and having high porosity while Sunmax granules were crushed granules and having low porosity. XRD analysis confirmed that Sunmax granules were biphasic hydroxyapatite and β-tricalcium phosphate while 3DP HA granules were monophasic hydroxyapatite. Sunmax granules exhibited greater agglomeration volume than that of 3DP HA granules. However, the water absorption of 3DP HA granules was greater than that of Sunmax granules. The greater agglomeration ability of Sunmax granules was likely due to the collagen constituent of the granules which could act as adhesive to bind granules together in addition to water capillary action. In contrast, 3DP HA granules formed the agglomeration by the water film due to the capillary action only so the efficiency was lower although the water absorption was greater.

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