A Simple and Environmentally Friendly Field Method for Fecal Analysis of Herbivore Diet

2020 ◽  
Vol 44 (4) ◽  
pp. 807-817
Author(s):  
Marie Fidele Tuyisenge ◽  
Winnie Eckardt ◽  
Samuel Nshutiyayesu ◽  
Melanie Devore
Keyword(s):  
Environmentally Friendly ◽  
Field Method ◽  
Fecal Analysis

Evaluation of the dark field method for large association of spread DNA

1978 ◽  
Vol 36 (2) ◽  
pp. 190-191
Author(s):  
Douglas C. Barker
Keyword(s):  
Electron Microscopy ◽  
Molecular Weight ◽  
Mitochondrial Dna ◽  
Extraction Method ◽  
Field Method ◽  
Dark Field ◽  
Kinetoplast Dna ◽  
Field Electron ◽  
Field Electron Microscopy ◽  
Dark Field Electron

A number of satisfactory methods are available for the electron microscopy of nicleic acids. These methods concentrated on fragments of nuclear, viral and mitochondrial DNA less than 50 megadaltons, on denaturation and heteroduplex mapping (Davies et al 1971) or on the interaction between proteins and DNA (Brack and Delain 1975). Less attention has been paid to the experimental criteria necessary for spreading and visualisation by dark field electron microscopy of large intact issociations of DNA. This communication will report on those criteria in relation to the ultrastructure of the (approx. 1 x 10-14g) DNA component of the kinetoplast from Trypanosomes. An extraction method has been developed to eliminate native endonucleases and nuclear contamination and to isolate the kinetoplast DNA (KDNA) as a compact network of high molecular weight. In collaboration with Dr. Ch. Brack (Basel [nstitute of Immunology), we studied the conditions necessary to prepare this KDNA Tor dark field electron microscopy using the microdrop spreading technique.

A Many-Beam Technique for Enhanced Resolution of Partial Dislocations

1972 ◽  
Vol 30 ◽  
pp. 646-647
Author(s):  
J. M. Oblak ◽  
B. H. Kear
Keyword(s):  
Phase Shift ◽  
Field Method ◽  
Dark Field ◽  
Bright Field ◽  
Field Technique ◽  
Weak Beam ◽  
Partial Dislocations ◽  
Enhanced Resolution ◽  
Nickel Base ◽  
Beam Technique

The “weak-beam” and systematic many-beam techniques are the currently available methods for resolution of closely spaced dislocations or other inhomogeneities imaged through strain contrast. The former is a dark field technique and image intensities are usually very weak. The latter is a bright field technique, but generally use of a high voltage instrument is required. In what follows a bright field method for obtaining enhanced resolution of partial dislocations at 100 KV accelerating potential will be described.A brief discussion of an application will first be given. A study of intermediate temperature creep processes in commercial nickel-base alloys strengthened by the Ll2 Ni3 Al γ precipitate has suggested that partial dislocations such as those labelled 1 and 2 in Fig. 1(a) are in reality composed of two closely spaced a/6 <112> Shockley partials. Stacking fault contrast, when present, tends to obscure resolution of the partials; thus, conditions for resolution must be chosen such that the phase shift at the fault is 0 or a multiple of 2π.

New Bronchodilator Joins Group of Environmentally Friendly Agents

2005 ◽  
Vol 38 (6) ◽  
pp. 67
Author(s):  
DAMIAN MCNAMARA
Keyword(s):  
Environmentally Friendly

CRITICAL PROPERTIES OF T. G. S. AND R. S. AS DETERMINED BY a d. c. ELECTRIC FIELD METHOD

1972 ◽  
Vol 33 (C2) ◽  
pp. C2-235-C2-236
Author(s):  
A. LEVSTIK ◽  
M. BURGAR ◽  
R. BLINC
Keyword(s):  
Electric Field ◽  
Field Method ◽  
Critical Properties ◽  
Electric Field Method

Are BMP Criteria Really Environmentally Friendly

WRPMD'99 ◽  
1999 ◽  
Author(s):  
Larry A. Roesner ◽  
Robert W. Brashear
Keyword(s):  
Environmentally Friendly

Environmentally Friendly Preparation of Magnetic Composites Featuring Proteolytic Properties

INEOS OPEN ◽  
2020 ◽  
Author(s):  
N. A. Samoilova ◽  
Keyword(s):  
Enzyme Immobilization ◽  
Maleic Acid ◽  
Environmentally Friendly ◽  
Synthetic Polymer ◽  
Magnetic Composites ◽  
Interpolyelectrolyte Complex ◽  
Hydrolysis Rates ◽  
Poly Ethylene ◽  
Noncovalent Binding

The enzyme-containing magnetic composites are presented. The magnetic matrix for enzyme immobilization is obtained by sequential application of an amine-containing polysaccharide—chitosan and a synthetic polymer—poly(ethylene-alt-maleic acid) to the magnetite microparticles to form the interpolyelectrolyte complex shell. Then, the enzyme (trypsin) is immobilized by covalent or noncovalent binding. Thus, the suggested composites can be readily obtained in the environmentally friendly manner. The enzyme capacity of the resulting composites reaches 28.0–32.6 mg/g. The maximum hydrolysis rates of the H-Val-Leu-Lys-pNA substrate provided by these composites range within 0.60·10–7–0.77·10–7 M/min.

Method of high frequency therapy at cows' mastitis during machine milking

2020 ◽  
pp. 9-12
Author(s):  
Viktor E. Lyubimov ◽  
Keyword(s):  
Dairy Cows ◽  
Electromagnetic Fields ◽  
High Frequency ◽  
High Efficiency ◽  
Environmentally Friendly ◽  
Ultrahigh Frequency ◽  
Machine Milking ◽  
High Frequency Electromagnetic Field ◽  
Milking Machine ◽  
Methods Of Treatment

Health of dairy cows ensures human health, so it is important that dairy products do not contain antibiotics that are used to treat any inflammation, including mastitis. In Russia at present, the problem of mastitis in cows exists with both attached and loose housing of cows. Mastitis of dairy cows are the great problem in milking husbandry. Losses from mastitis in milk yield can reach 15-20%. The main reasons for the occurrence of nonspecific mastitis of cows are the shortcomings of the working components of the milking machine: the degree of deterioration of the nipple rubber and the violation of machine milking technology (the reduction in pre-milking time and vacuum fluctuations account for 70% of all causes). Treatment of mastitis with antibiotics is effective, but not environmentally friendly and unacceptable. For the treatment of inflammation of the udder, it is necessary to use more environmentally friendly methods of treatment, one of which is the exposure to ultra-high frequency electromagnetic field tested by medicine. Use of three types of medical-mobile milking machines with the same method of exposure to ultrahigh-frequency electromagnetic fields on cow's nipples through electrodes in milking cups: LPDA-1-UHF, LPDA-2-UHF and LPDA-UHF-30 M, is described in the article. Author proved that cows with subclinical forms of mastitis recovered faster during milking with exposure to the ultrahigh frequency than when treated by antibiotics, and milking with the ultrahigh frequency device helps to recover 82% of the affected quarters with clinical forms and 100% of cows with subclinical forms of mastitis or with udder irritation. The high efficiency of the method of exposure to electromagnetic fields of the ultrahigh frequency during machine milking by means of medical-mobile milking machines LPDA-UHF for the prevention and treatment of mastitis of cows was shown.

Environmentally friendly polymer nanocomposites

2013 ◽  
Author(s):  
Suprakas Sinha Ray
Keyword(s):  
Polymer Nanocomposites ◽  
Environmentally Friendly
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