Biocontrol ability and action mechanisms of Aureobasidium pullulans GE17 and Meyerozyma guilliermondii KL3 against Penicillium digitatum DSM2750 and Penicillium expansum DSM62841 causing postharvest diseases

Yeast ◽  
2020 ◽  
Vol 37 (9-10) ◽  
pp. 437-448
Author(s):  
Bilal Agirman ◽  
Huseyin Erten
Keyword(s):  
Aureobasidium Pullulans ◽  
Penicillium Digitatum ◽  
Penicillium Expansum ◽  
Postharvest Diseases ◽  
Meyerozyma Guilliermondii ◽  
Action Mechanisms

Infection capacities in the orange-pathogen relationship: Compatible (Penicillium digitatum) and incompatible (Penicillium expansum) interactions

2012 ◽  
Vol 29 (1) ◽  
pp. 56-66 ◽  
Author(s):  
L. Vilanova ◽  
I. Viñas ◽  
R. Torres ◽  
J. Usall ◽  
A.M. Jauset ◽  
...  
Keyword(s):  
Penicillium Digitatum ◽  
Penicillium Expansum

SUGAR VARIATION IN 'ROCHA' PEARS AND PENICILLIUM EXPANSUM REDUCTION BY AUREOBASIDIUM PULLULANS

2008 ◽  
pp. 929-934
Author(s):  
I.P. Pais ◽  
A.P. Ramos ◽  
M.G. Barreiro ◽  
M.J. Silva
Keyword(s):  
Aureobasidium Pullulans ◽  
Penicillium Expansum

scholarly journals A Novel Secreted Cysteine-Rich Anionic (Sca) Protein from the Citrus Postharvest Pathogen Penicillium digitatum Enhances Virulence and Modulates the Activity of the Antifungal Protein B (AfpB)

2020 ◽  
Vol 6 (4) ◽  
pp. 203
Author(s):  
Sandra Garrigues ◽  
Jose F. Marcos ◽  
Paloma Manzanares ◽  
Mónica Gandía
Keyword(s):  
Citrus Fruit ◽  
Penicillium Digitatum ◽  
Antifungal Protein ◽  
Penicillium Expansum ◽  
Wild Type ◽  
Phenotypic Differences ◽  
Antifungal Proteins ◽  
Ascomycete Fungi ◽  
In Vitro Antifungal Activity

Antifungal proteins (AFPs) from ascomycete fungi could help the development of antimycotics. However, little is known about their biological role or functional interactions with other fungal biomolecules. We previously reported that AfpB from the postharvest pathogen Penicillium digitatum cannot be detected in the parental fungus yet is abundantly produced biotechnologically. While aiming to detect AfpB, we identified a conserved and novel small Secreted Cysteine-rich Anionic (Sca) protein, encoded by the gene PDIG_23520 from P. digitatum CECT 20796. The sca gene is expressed during culture and early during citrus fruit infection. Both null mutant (Δsca) and Sca overproducer (Scaop) strains show no phenotypic differences from the wild type. Sca is not antimicrobial but potentiates P. digitatum growth when added in high amounts and enhances the in vitro antifungal activity of AfpB. The Scaop strain shows increased incidence of infection in citrus fruit, similar to the addition of purified Sca to the wild-type inoculum. Sca compensates and overcomes the protective effect of AfpB and the antifungal protein PeAfpA from the apple pathogen Penicillium expansum in fruit inoculations. Our study shows that Sca is a novel protein that enhances the growth and virulence of its parental fungus and modulates the activity of AFPs.

Effects of Pichia guilliermondii and Hot Air Treatment on the Postharvest Preservation of Red Fuji Apple Quality Attributes

2018 ◽  
Vol 81 (2) ◽  
pp. 186-194 ◽  
Author(s):  
Yan Zhao ◽  
Jingjing Yin
Keyword(s):  
Apple Fruit ◽  
Penicillium Expansum ◽  
Total Phenolic ◽  
Postharvest Diseases ◽  
Pichia Guilliermondii ◽  
Air Treatment ◽  
Hot Air ◽  
Antagonistic Yeast ◽  
Hot Air Treatment ◽  
Apple Quality

ABSTRACT The effects of individual and combined Pichia guilliermondii (at 1 × 108 CFU mL−1) and hot air (at 38°C for 96 h) treatments on the three major postharvest diseases Botrytis cinerea, Penicillium expansum, and Colletotrichum gloeosporioides, as well as the quality and antioxidant content of Red Fuji (Malus pumila var. domestica) apple fruit, were investigated in this work. Results suggested that the combined hot air and antagonistic yeast (P. guilliermondii) treatment effectively and completely inhibited the infection of apple fruit wounds by the three major postharvest diseases. Furthermore, apple fruit treated with antagonistic yeast or heat alone maintained better quality, which included mass loss, firmness, solid/acid ratio, and ascorbic acid content, than the control. The combination of the two treatments yielded the optimum apple quality. Moreover, the combined hot air and P. guilliermondii treatment also maintained or enhanced the antioxidative enzyme activities and total phenolic content of apple fruit. All results demonstrated that the combined antagonistic yeast and hot air treatment maintained the postharvest freshness of apple fruit.

Comparison of thiabendazole and benomyl as post-harvest fungicides for wastage control in long term lemon storage

1975 ◽  
Vol 15 (72) ◽  
pp. 108 ◽  
Author(s):  
BL Wild ◽  
LE Rippon ◽  
JA Seberry
Keyword(s):  
Penicillium Digitatum ◽  
Skin Colour ◽  
Postharvest Diseases ◽  
Ambient Temperatures ◽  
Post Harvest ◽  
Deep Yellow ◽  
Green Mould

Thiabendazole and benomyl were compared for their effectiveness in controlling major postharvest diseases of lemons, caused by green mould (Penicillium digitatum Sacc.) and stem-end rot (Diaporthe citri (Faw.) Wolf). The lemons were stored at ambient temperatures and at 12�C and examined over a period of 26 weeks. Results showed benomyl gave excellent and better control of the diseases than TBZ. Although benomyl treated fruit was sound, skin colour was deep yellow and unattractive indicating the need for further research into methods of improving fruit appearance during long periods of storage.

scholarly journals Production, formulation and antagonistic activity of the biocontrol like-yeast Aureobasidium pullulans against Penicillium expansum

2007 ◽  
Vol 29 (4) ◽  
pp. 553-559 ◽  
Author(s):  
R. Mounir ◽  
A. Durieux ◽  
E. Bodo ◽  
C. Allard ◽  
J.-P. Simon ◽  
...  
Keyword(s):  
Aureobasidium Pullulans ◽  
Antagonistic Activity ◽  
Penicillium Expansum

scholarly journals Control of Postharvest Pathogens and Colonization of the Apple Surface by Antagonistic Microorganisms in the Field

1997 ◽  
Vol 87 (11) ◽  
pp. 1103-1110 ◽  
Author(s):  
Wolfgang Leibinger ◽  
Barbara Breuker ◽  
Matthias Hahn ◽  
Kurt Mendgen
Keyword(s):  
Aureobasidium Pullulans ◽  
Random Amplified Polymorphic Dna ◽  
Rhodotorula Glutinis ◽  
Growing Season ◽  
Penicillium Expansum ◽  
Promising Alternative ◽  
Antagonistic Microorganisms ◽  
Fruit Decay ◽  
Population Sizes ◽  
Control Post

Selected isolates of Aureobasidium pullulans, Rhodotorula glutinis, and Bacillus subtilis reduced the size and number of lesions on wounded apples caused by the postharvest pathogens Penicillium expansum, Botrytis cinerea, and Pezicula malicorticis. Combinations of the antagonistic microorganisms were applied to apple trees in the field late in the growing season of two consecutive years. The population dynamics of the introduced microorganisms and the incidence of fruit decay were determined. Population sizes of introduced antagonists on apple surfaces increased in the field following application of treatments until harvest. After transfer of the fruit from the field into cold storage, the populations of the introduced antagonists remained higher than in the control treatments. Identification of the applied isolates of A. pullulans and R. glutinis during the experiments was achieved by isolate-specific DNA probes generated from random amplified polymorphic DNA. A combination of two strains of A. pullulans and one strain of R. glutinis suppressed rotting of apple to the same extent as the commonly used fungicide Euparen. Our data demonstrate that the application of antagonistic microorganisms in the field represents a promising alternative to fungicide treatments to control post-harvest diseases of apple.

ANTAGONIST ACTIVITY BETWEEN AUREOBASIDIUM PULLULANS AND PENICILLIUM EXPANSUM IN PYRUS COMMUNIS L. 'ROCHA' - IMPLICATIONS ON THE RESISTANCE TO OXIDATIVE STRESS

2012 ◽  
pp. 347-354
Author(s):  
F.J.C. Lidon ◽  
J.C. Ramalho ◽  
J.D. Arrabaça ◽  
I.P. Pais ◽  
C.S. Carvalho ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Aureobasidium Pullulans ◽  
Pyrus Communis ◽  
Penicillium Expansum ◽  
Antagonist Activity ◽  
Pyrus Communis L

Pyrimethanil Sensitivity and Resistance Mechanisms in Penicillium digitatum

Plant Disease ◽  
2020 ◽  
Author(s):  
Yuchao Zhang ◽  
Yanping Fu ◽  
Chaoxi Luo ◽  
Fuxing Zhu
Keyword(s):  
Target Genes ◽  
Sodium Dodecyl ◽  
Resistance Mechanisms ◽  
Penicillium Digitatum ◽  
Cross Resistance ◽  
Postharvest Diseases ◽  
Cell Wall Degrading Enzymes ◽  
Baseline Sensitivity ◽  
Nucleotide Mutation ◽  
Resistant Mutants

Pyrimethanil is an anilinopyrimidines (AP) fungicide and highly effective in controlling green mold caused by Penicillium digitatum but has not yet been registered in China to control postharvest diseases of citrus. In the present study, baseline sensitivity of P. digitatum to pyrimethanil was established based on the effective concentrations for 50% inhibition (EC50) values of 127 isolates collected from five major citrus-growing regions of China. The distribution of these EC50 values was unimodal but with a long right tail. The mean EC50 value was 0.137 ± 0.046 μg/mL (SD), and the minimum and maximum were 0.073 and 0.436 μg/mL, respectively. Pyrimethanil in potato dextrose agar (PDA) at 0.20 μg/mL decreased methionine production in the mycelia by 21.6% and reduced the activities of cell wall-degrading enzymes cellulase and pectinase by 9.1 and 32.8%, respectively. Twelve pyrimethanil-resistant mutants were obtained by consecutive sub-culturing of 12 arbitrarily selected sensitive isolates on pyrimethanil-amended PDA for 4 generations, and the resistance factors ranged from 69 to 3421. There was no cross-resistance between pyrimethanil and prochloraz (r = 0.377, P = 0.123). Compared with their parental isolates, pyrimethanil-resistant mutants had reduced pathogenicity to citrus fruit but higher tolerance to hydrogen peroxide. No differences were detected in tolerance to NaCl, CaCl2, Congo red, or sodium dodecyl sulfate (SDS). Exogenous addition of methionine into PDA partially alleviated the toxicity of pyrimethanil to the sensitive isolates but had no significant effect on toxicity to the resistant mutants. Sequencing of cystathionine γ-synthase encoding genes CGS1 and CGS2, the potential target genes for pyrimethanil, showed that there was no nucleotide mutation in the coding region of CGS of the pyrimethanil-resistant mutants. However, the relative expression of CGS1 and CGS2 genes of the pyrimethanil-resistant mutants was reduced by 42.5 and 57.4%, respectively. These results have important implications for applications of pyrimethanil to control P. digitatum and for understanding the modes of action and resistance mechanisms of pyrimethanil.

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