Absence of MMF1 disrupts heme biosynthesis by targeting Hem1p in Saccharomyces cerevisiae

Yeast ◽  
2021 ◽  
Author(s):  
Gregory H. Whitaker ◽  
Dustin C. Ernst ◽  
Diana M. Downs
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Heme Biosynthesis

scholarly journals Novel Cell-Killing Mechanisms of Hydroxyurea and the Implication toward Combination Therapy for the Treatment of Fungal Infections

2017 ◽  
Vol 61 (11) ◽  
Author(s):  
Amanpreet Singh ◽  
Ameeta Agarwal ◽  
Yong-jie Xu
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Fungal Pathogen ◽  
Fungal Infections ◽  
Genetic Data ◽  
Heme Biosynthesis ◽  
Wild Type ◽  
Content Type ◽  
Ergosterol Synthesis ◽  
Opportunistic Fungal Pathogen ◽  
Cell Lethality

ABSTRACT We have previously reported that an erg11 mutation affecting ergosterol synthesis and a hem13 mutation in the heme synthesis pathway significantly sensitize the fission yeast Schizosaccharomyces pombe to hydroxyurea (HU) (1, 2). Here we show that treatment with inhibitors of Erg11 and heme biosynthesis phenocopies the two mutations in sensitizing wild-type cells to HU. Importantly, HU synergistically interacts with the heme biosynthesis inhibitor sampangine and several Erg11 inhibitors, the antifungal azoles, in causing cell lethality. Since the synergistic drug interactions are also observed in the phylogenetically divergent Saccharomyces cerevisiae and the opportunistic fungal pathogen Candida albicans, the synergism is likely conserved in eukaryotes. Interestingly, our genetic data for S. pombe has also led to the discovery of a robust synergism between sampangine and the azoles in C. albicans. Thus, combinations of HU, sampangine, and the azoles can be further studied as a new method for the treatment of fungal infections.

A conserved cysteine residue in yeast uroporphyrinogen decarboxylase is not essential for enzymatic activity

1997 ◽  
Vol 43 (8) ◽  
pp. 792-795 ◽  
Author(s):  
Celestino Di Flumeri ◽  
Nicholas H. Acheson ◽  
Teresa Keng
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Enzymatic Activity ◽  
Mutant Strain ◽  
Cysteine Residue ◽  
Catalytic Site ◽  
Heme Biosynthesis ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Decarboxylase Activity ◽  
Uroporphyrinogen Decarboxylase

Uroporphyrinogen decarboxylase catalyzes the fifth step of heme biosynthesis in Saccharomyces cerevisiae. Studies utilizing sulfhydryl-specific reagents suggest that the enzyme requires a cysteine residue within the catalytic site This hypothesis was tested directly by site-directed mutagenesis of highly conserved cysteine-52 to serine or alanine. Plasmids containing these mutations were able to complement a hem6 mutant strain. In addition, properties associated with decreased uroporphyrinogen decarboxylase activity were not detected in the mutant strain transformed with these mutant plasmids. These results suggest that the conserved cysteine-52 by itself is not essential for enzymatic activity.Key words: heme biosynthesis, uroporphyrinogen decarboxylase, site-directed mutagenesis.

scholarly journals Anaerobic growth of Saccharomyces cerevisiae alleviates the lethal effect of phosphotyrosyl phosphatase activators depletion.

2001 ◽  
Vol 48 (4) ◽  
pp. 1043-1049
Author(s):  
B Rempola ◽  
A Kaniak ◽  
J P Di Rago ◽  
J Rytka
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Mitochondrial Genome ◽  
Lethal Effect ◽  
Heme Biosynthesis ◽  
Anaerobic Growth ◽  
Double Mutation ◽  
Synthetic Lethal ◽  
Per Se

Saccharomyces cerevisiae homologues of phosphotyrosyl phosphatase activator (PTPA) are encoded byRRD1 and RRD2, genes whose combined deletion is synthetic lethal. Previously we have shown that the lethality of rrd1,2delta can be suppressed by increasing the osmolarity of the medium. Here we show that the lethality of rrd1,2delta is also suppressed under oxygen-limited conditions. The absence of respiration per se is not responsible for the suppression since elimination of the mitochondrial genome or a block in heme biosynthesis fail to rescue the rrd1,2delta double mutation.

scholarly journals Involvement of heme biosynthesis in control of sterol uptake by Saccharomyces cerevisiae.

1985 ◽  
Vol 163 (1) ◽  
pp. 199-207 ◽  
Author(s):  
T A Lewis ◽  
F R Taylor ◽  
L W Parks
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Heme Biosynthesis ◽  
Sterol Uptake

scholarly journals Balanced globin protein expression and heme biosynthesis improve production of human hemoglobin in Saccharomyces cerevisiae

2014 ◽  
Vol 21 ◽  
pp. 9-16 ◽  
Author(s):  
Lifang Liu ◽  
José L. Martínez ◽  
Zihe Liu ◽  
Dina Petranovic ◽  
Jens Nielsen
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Protein Expression ◽  
Heme Biosynthesis ◽  
Human Hemoglobin ◽  
Improve Production

scholarly journals EFFECTS OF GLUCOSE ON HEME BIOSYNTHESIS OF SACCHAROMYCES CEREVISIAE

1966 ◽  
Vol 15 (1) ◽  
pp. 51-53
Author(s):  
TOMOMI SAKURADA ◽  
HIROKO MATSUMURA
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Heme Biosynthesis

Anti-Saccharomyces cerevisiae Antibodies in Inflammatory Bowel Disease: a Family Study

2001 ◽  
Vol 36 (2) ◽  
pp. 196-201 ◽  
Author(s):  
F. Seibold ◽  
O. Stich ◽  
R. Hufnagl ◽  
S. Kamil ◽  
M. Scheurlen
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Inflammatory Bowel Disease ◽  
Bowel Disease ◽  
Family Study ◽  
Inflammatory Bowel

Antioxidant evaluation of Helichrysum sp. and Santolina sp. extracts on Saccharomyces cerevisiae cell model

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
N Grčić ◽  
R Oliveira ◽  
ACP Dias
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Model ◽  
Antioxidant Evaluation
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