TNF-α Stimulation of MCP-1 Expression Is Mediated by the Akt/PKB Signal Transduction Pathway in Vascular Endothelial Cells

2000 ◽  
Vol 276 (2) ◽  
pp. 791-796 ◽  
Author(s):  
Koji Murao ◽  
Tomoyo Ohyama ◽  
Hitomi Imachi ◽  
Toshihiko Ishida ◽  
Wen Ming Cao ◽  
...  
Keyword(s):  
Signal Transduction ◽  
Endothelial Cells ◽  
Vascular Endothelial Cells ◽  
Signal Transduction Pathway ◽  
Vascular Endothelial ◽  
Transduction Pathway ◽  
Tnf Α ◽  
Stimulation Of

scholarly journals Urokinase Activates the Jak/Stat Signal Transduction Pathway in Human Vascular Endothelial Cells

1999 ◽  
Vol 19 (2) ◽  
pp. 290-297 ◽  
Author(s):  
Inna Dumler ◽  
Angela Kopmann ◽  
Angelika Weis ◽  
Oleg A. Mayboroda ◽  
Kai Wagner ◽  
...  
Keyword(s):  
Signal Transduction ◽  
Endothelial Cells ◽  
Vascular Endothelial Cells ◽  
Signal Transduction Pathway ◽  
Vascular Endothelial ◽  
Transduction Pathway

Signal Transduction of Vascular Endothelial Cells

1995 ◽  
Vol 6 (2) ◽  
pp. 67-73
Author(s):  
Michihiko KUWANO ◽  
Hiroto IZUMI ◽  
Tadahisa SHONO ◽  
Sei-ichiro JIMI ◽  
Yukihiro WAKABAYASHI ◽  
...  
Keyword(s):  
Signal Transduction ◽  
Endothelial Cells ◽  
Vascular Endothelial Cells ◽  
Vascular Endothelial

Heterogeneity of the signal transduction pathways for VEGF-induced MAPKs activation in human vascular endothelial cells

2001 ◽  
Vol 188 (2) ◽  
pp. 201-210 ◽  
Author(s):  
Rei Yashima ◽  
Mayumi Abe ◽  
Katsuhiro Tanaka ◽  
Hikaru Ueno ◽  
Kenya Shitara ◽  
...  
Keyword(s):  
Signal Transduction ◽  
Endothelial Cells ◽  
Vascular Endothelial Cells ◽  
Signal Transduction Pathways ◽  
Vascular Endothelial

scholarly journals GAS6 Inhibits Granulocyte Adhesion to Endothelial Cells

Blood ◽  
1998 ◽  
Vol 91 (7) ◽  
pp. 2334-2340
Author(s):  
Gian Carlo Avanzi ◽  
Margherita Gallicchio ◽  
Flavia Bottarel ◽  
Loretta Gammaitoni ◽  
Giuliana Cavalloni ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Vascular Endothelial Cells ◽  
Platelet Activating Factor ◽  
Polymorphonuclear Cells ◽  
Vascular Endothelial ◽  
Tnf Α ◽  
High Concentrations ◽  
Adhesive Interactions ◽  
Factor Α ◽  
Dose Dependent

GAS6 is a ligand for the tyrosine kinase receptors Rse, Axl, and Mer, but its function is poorly understood. Previous studies reported that both GAS6 and Axl are expressed by vascular endothelial cells (EC), which play a key role in leukocyte extravasation into tissues during inflammation through adhesive interactions with these cells. The aim of this work was to evaluate the GAS6 effect on the adhesive function of EC. Treatment of EC with GAS6 significantly inhibited adhesion of polymorphonuclear cells (PMN) induced by phorbol 12-myristate 13-acetate (PMA), platelet-activating factor (PAF), thrombin, interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α), but not that induced by FMLP and IL-8. GAS6 did not affect adhesion to resting EC. Titration experiments showed that high concentrations of GAS6 were needed to inhibit PMN adhesion and that inhibition was dose-dependent at the concentration range of 0.1 to 1 μg/mL. One possibility was that high concentrations were needed to overwhelm the effect of endogenous GAS6 produced by EC. In line with this possibility, treatment of resting EC with soluble Axl significantly potentiated PMN adhesion. Analysis of localization of GAS6 by confocal microscopy and cytofluorimetric analysis showed that it is concentrated along the plasma membrane in resting EC and treatment with PAF induces depletion and/or redistribution of the molecule. These data suggest that GAS6 functions as a physiologic antiinflammatory agent produced by resting EC and depleted when proinflammatory stimuli turn on the proadhesive machinery of EC.

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