A Chemically Defined Medium Supports in Vitro Proliferation and Maintains the Osteochondral Potential of Rat Marrow-Derived Mesenchymal Stem Cells

1995 ◽  
Vol 219 (1) ◽  
pp. 211-222 ◽  
Author(s):  
Donald P. Lennon ◽  
Stephen E. Haynesworth ◽  
Randell G. Young ◽  
James E. Dennis ◽  
Arnold I. Caplan
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Defined Medium ◽  
Chemically Defined Medium ◽  
In Vitro Proliferation

scholarly journals Effects of antithrombotic drugs fondaparinux and tinzaparin on in vitro proliferation and osteogenic and chondrogenic differentiation of bone‐derived mesenchymal stem cells

2011 ◽  
Vol 29 (9) ◽  
pp. 1327-1335 ◽  
Author(s):  
Argiris Papathanasopoulos ◽  
Dimitrios Kouroupis ◽  
Karen Henshaw ◽  
Dennis McGonagle ◽  
Elena A. Jones ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Chondrogenic Differentiation ◽  
Antithrombotic Drugs ◽  
In Vitro Proliferation

scholarly journals In vitro proliferation and differentiation of canine bone marrow derived mesenchymal stem cells over hydroxyl functionalized CNT substrates

2019 ◽  
Vol 24 ◽  
pp. e00387
Author(s):  
A.P. Madhusoodan ◽  
Kinsuk Das ◽  
Bhabesh Mili ◽  
Kuldeep Kumar ◽  
Ajay Kumar ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
In Vitro Proliferation ◽  
Proliferation And Differentiation

scholarly journals In vitro Proliferation of Human Bone Marrow Mesenchymal Stem Cells Employing Donor Serum and Basic Fibroblast Growth Factor

2003 ◽  
Vol 43 (1-3) ◽  
pp. 89-96 ◽  
Author(s):  
Mutsumi Takagi ◽  
Tetsuya Nakamura ◽  
Chikayoski Matsuda ◽  
Takako Hattori ◽  
Shigeyuki Wakitani ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Basic Fibroblast Growth Factor ◽  
Fibroblast Growth ◽  
In Vitro Proliferation ◽  
Marrow Mesenchymal Stem Cells

scholarly journals Leukocyte-reduced platelet-rich plasma stimulates the in vitro proliferation of adipose-tissue derived mesenchymal stem cells depending on PDGF signaling

2017 ◽  
Vol 67 (2) ◽  
pp. 183-196 ◽  
Author(s):  
Siegmund Lang ◽  
Marietta Herrmann ◽  
Christian Pfeifer ◽  
Gero Brockhoff ◽  
Johannes Zellner ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Platelet Rich Plasma ◽  
In Vitro Proliferation ◽  
Pdgf Signaling

Therapeutic applications of mesenchymal stem cells: A comprehensive review

2020 ◽  
Vol 15 ◽  
Author(s):  
Pouria Samadi ◽  
Sahar Saki ◽  
Hamed Manoochehri Khoshinani ◽  
Mohsen Sheykhhasan
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Mhc Class Ii ◽  
Clinical Studies ◽  
Adult Stem Cells ◽  
Embryonic Stem ◽  
In Vitro Proliferation ◽  
Induced Pluripotent ◽  
Medical Indications

: Mesenchymal stem cells (MSCs) are one of the most common types of adult stem cells. While MSCs are traditionally isolated from bone marrow, over the last few years, they have also been found in many other adult tissues such as liver, cord blood, placenta, dental pulp and adipose tissue. They have been investigated as a marvelous cell source for tissue regeneration and suggested as a therapy in non-autologous application, because of lack of MHC class II expression. For the past several decades, furthermore, MSCs show promise as a therapeutic strategy in medicine. Many advantages such as self-renewal, in vitro proliferation, rapid cell doubling capacity, easy of GMP manufacturing, antifibrotic, anti-apoptotic, anti-inflammation, immunomodulatory and immunosuppressive effects, and paracrine nature have been demonstrated in various pre-clinical studies and clinical evidences. The ability of MSCs to differentiate into different cell lineages, in addition to the lack of ethical problems in comparison with embryonic stem cells as well as induced Pluripotent Stem cells (iPSCs), have attracted much attention. Due to their unique features, various medical indications such as therapeutic medicine, tissue engineering, and cell therapy have allowed the development and flourishment of MSCs. The various different clinical trials were performed using MSCs for the treatment of a long list of diseases and disorders. Results of these clinical studies have demonstrated the capability of MSCs to be used for the treatment of dermatological, musculoskeletal, neurological, cardiovascular, respiratory, renal, gastroenterological and urological conditions, etc.

scholarly journals Attachment, Growth, and Detachment of Human Mesenchymal Stem Cells in a Chemically Defined Medium

2016 ◽  
Vol 2016 ◽  
pp. 1-10 ◽  
Author(s):  
Denise Salzig ◽  
Jasmin Leber ◽  
Katharina Merkewitz ◽  
Michaela C. Lange ◽  
Natascha Köster ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Collagen Type ◽  
Human Mesenchymal Stem Cells ◽  
Defined Medium ◽  
Growth Surface ◽  
Lag Phase ◽  
Collagen Type Iv ◽  
Chemically Defined Medium ◽  
Type Iv

The manufacture of human mesenchymal stem cells (hMSCs) for clinical applications requires an appropriate growth surface and an optimized, preferably chemically defined medium (CDM) for expansion. We investigated a new protein/peptide-free CDM that supports the adhesion, growth, and detachment of an immortalized hMSC line (hMSC-TERT) as well as primary cells derived from bone marrow (bm-hMSCs) and adipose tissue (ad-hMSCs). We observed the rapid attachment and spreading of hMSC-TERT cells and ad-hMSCs in CDM concomitant with the expression of integrin and actin fibers. Cell spreading was promoted by coating the growth surface with collagen type IV and fibronectin. The growth of hMSC-TERT cells was similar in CDM and serum-containing medium whereas the lag phase of bm-hMSCs was prolonged in CDM. FGF-2 or surface coating with collagen type IV promoted the growth of bm-hMSCs, but laminin had no effect. All three cell types retained their trilineage differentiation capability in CDM and were detached by several enzymes (but not collagenase in the case of hMSC-TERT cells). The medium and coating did not affect detachment efficiency but influenced cell survival after detachment. CDM combined with cell-specific surface coatings and/or FGF-2 supplements is therefore as effective as serum-containing medium for the manufacture of different hMSC types.

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