Effects of Extracellular Matrix on Phenotype Modulation and MAPK Transduction of Rat Aortic Smooth Muscle Cells in Vitro

2000 ◽  
Vol 69 (2) ◽  
pp. 79-90 ◽  
Author(s):  
Hanjuan Qin ◽  
Toshiyuki Ishiwata ◽  
Roujiao Wang ◽  
Mitsuhiro Kudo ◽  
Munehiro Yokoyama ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Aortic Smooth Muscle Cells ◽  
Aortic Smooth Muscle

Density-dependent hyperpolarization in cultured aortic smooth muscle cells

1989 ◽  
Vol 256 (3) ◽  
pp. C644-C651 ◽  
Author(s):  
M. G. Blennerhassett ◽  
M. S. Kannan ◽  
R. E. Garfield
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Continuous Process ◽  
Primary Cultures ◽  
Muscle Cells ◽  
Sprague Dawley ◽  
Aortic Smooth Muscle Cells ◽  
Aortic Smooth Muscle ◽  
Wistar Kyoto

The membrane potential (Em) of cultured aortic smooth muscle cells from Sprague-Dawley (SD), Wistar-Kyoto (WKY), and spontaneously hypertensive (SHR) rats was measured in proliferating primary cultures. Em of SD cells in high-density cultures was -51 to -58 mV, whereas that of low-density cultures (1-2 days) was -30 mV. This difference was due to a continuous process of hyperpolarization during proliferation in culture. Em of WKY and SHR hyperpolarized similarly, from -12 to -42 and -38 mV, respectively. Hyperpolarization of Em of SD, WKY, and SHR cells was related to cell density rather than time in culture. Em may be a sensitive and significant indicator of the changes in the differentiated state expressed by proliferating smooth muscle in vitro.

scholarly journals Unusual lysosomes in aortic smooth muscle cells: presence in living and rapidly frozen cells.

1986 ◽  
Vol 102 (5) ◽  
pp. 1615-1622 ◽  
Author(s):  
J M Robinson ◽  
T Okada ◽  
J J Castellot ◽  
M J Karnovsky
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Lucifer Yellow ◽  
Muscle Cells ◽  
Rapid Freezing ◽  
Tubular Structures ◽  
Aortic Smooth Muscle Cells ◽  
Aortic Smooth Muscle ◽  
Ultrastructural Appearance

Unusual tubular structures have been observed in rat aortic smooth muscle cells (SMC) grown in culture. These tubular structures have several characteristics that strongly suggest that they are lysosomes: they are bounded by a single membrane bilayer, contain densely staining material, and acid phosphatase activity. Furthermore, these structures are present in living cells, as demonstrated by their ability to accumulate the membrane-impermeable fluorescent dye lucifer yellow CH. In ultrastructural preparations they are best seen in samples that are cryofixed by rapid freezing and then freeze-substituted in osmium-acetone solutions. Conventional chemical fixation did not appear to preserve these structures to as great an extent as did rapid freezing. Comparison of SMC in vitro to the same cells in situ revealed differences in lysosome number as well as morphological appearance. Thus, the culturing of rat SMC leads to the formation of unusual tubular lysosomes whose ultrastructural appearance is particularly sensitive to the methods employed for examination.

scholarly journals Angiotensin II modulates calponin mRNA expression in aortic smooth muscle cells in vitro and in vivo

1998 ◽  
Vol 31 ◽  
pp. 104
Author(s):  
W.M.M. van de Greef ◽  
G. Castoldi ◽  
C.R.T. di Gioia ◽  
F. Clementi ◽  
M.-R. Camellitti ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Angiotensin Ii ◽  
Smooth Muscle Cells ◽  
Mrna Expression ◽  
Muscle Cells ◽  
Aortic Smooth Muscle Cells ◽  
Aortic Smooth Muscle

The Effects Of Thrombospondin-2 Silencing In Human Aortic Smooth Muscle Cells In-Vitro

2011 ◽  
Vol 165 (2) ◽  
pp. 253
Author(s):  
S. Yoshida ◽  
C.S. Nabzdyk ◽  
M.C. Chun ◽  
L. Pradhan ◽  
F.W. LoGerfo
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Aortic Smooth Muscle Cells ◽  
Aortic Smooth Muscle
Sign in / Sign up

Export Citation Format

Share Document