Total Fatty Acid Content Determination of Whole Microalgal Biomass Using In Situ Transesterification

Author(s):  
S. Van Wychen ◽  
L. M. L. Laurens
Crustaceana ◽  
2021 ◽  
Vol 94 (1) ◽  
pp. 29-43
Author(s):  
Yu Wang ◽  
Guangguang Li ◽  
Zhen Han ◽  
Guangwei Hu ◽  
Binlun Yan ◽  
...  

Abstract Prawns of the species Exopalaemon carinicauda were fed with Artemia that had been enriched by either 1, or 3, or 5 g/l DHA powder. The feeding dose of Artemia offered to E. carinicauda was calculated according to the water volume, and maintained at 1-3 individuals/ml. After nutritional enrichment, the ratio of DHA content to total fatty acid content in Artemia was the highest (3.32%) after being fed 3 g/l DHA powder. The ratio of DHA content to total fatty acid content in the muscle tissue of E. carinicauda prawns fed with Artemia enhanced with 3 g/l DHA powder was also the highest (4.40%) achieved. Furthermore, when the concentration of fish oil DHA powder was 3 g/l, the survival rate of the prawns was significantly higher than in the control group, with the specific growth rate and weight gain reaching 11.39%/day, and 95.74%, respectively. In addition, the DHA-enhanced Artemia, at different concentrations, increased the non-specific immunity of individuals. The enzyme activity of T-AOC, SOD and AKP in the respective feeding groups of prawns was significantly higher, compared to the control group. Thus, Artemia enriched by 3 g/l DHA powder is proposed as an optimal choice for the offspring breeding of E. carinicauda.


2012 ◽  
Vol 41 (2) ◽  
pp. 161-171 ◽  
Author(s):  
N. Parunović ◽  
M. Petrović ◽  
V. Matekalo-Sverak ◽  
D. Radojković ◽  
D. Vranić ◽  
...  

1971 ◽  
Vol 125 (4) ◽  
pp. 963-969 ◽  
Author(s):  
T. Nurminen ◽  
H. Suomalainen

1. The total yield of fatty acids from the whole envelopes was markedly higher than that obtained from the ordinary cell walls. In both samples the major fatty acids were C16 and C18 acids. 2. The whole envelopes contained C18 acids and long-chain (C19–C26) fatty acids, in a higher proportion than did the ordinary cell walls. Fifteen fatty acids with more than 18 carbon atoms were identified, among which 2-hydroxy-C26:0 and C26:0 acids predominated. 3. A complex sphingolipid containing inositol, phosphorus and mannose was isolated from the whole cell envelopes. The main fatty acids of this lipid were 2-hydroxy-C26:0 and C26:0 acids. It was concluded that this sphingolipid is present both in the ordinary cell wall and in the plasma membrane of baker's yeast. 4. The neutral lipids amounted to over 50% and the glycerophosphatides to about 30% of the total fatty acid content of the whole envelope. The major fatty acids in these lipids were C16:1, C18:1 and C16:0 acids. The proportion of fatty acids with more than 18 carbon atoms was lowest in the neutral lipids, whereas the neutral glycolipids contained the highest percentage of these fatty acids. Acidic glycolipids amounted to 14% of the total fatty acid content of the whole envelope. The presence of a cerebroside sulphate in this lipid fraction was demonstrated, whereas the high content of 2-hydroxy-C26:0 acid found is caused by the complex inositol- and mannose-containing sphingolipid.


2017 ◽  
Vol 119 (10) ◽  
pp. 1700043 ◽  
Author(s):  
Caroline Mariana de Aguiar ◽  
Silvio Cesar Sampaio ◽  
Kátia Andressa Santos ◽  
Edson Antônio da Silva ◽  
Pitágoras Augusto Piana ◽  
...  

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