A Simple Method of Generating 3D Brain Organoids Using Standard Laboratory Equipment

2017 ◽  
pp. 1-12 ◽  
Author(s):  
Magdalena Sutcliffe ◽  
Madeline A. Lancaster
Keyword(s):  
Standard Laboratory ◽  
Laboratory Equipment ◽  
Simple Method

Disinfection Capability in Water for Swimming and Bathing Pools: A Simple Method for Their Evaluation in Practice

1991 ◽  
Vol 24 (2) ◽  
pp. 359-362 ◽  
Author(s):  
K. M. Seidel ◽  
J. M. Lopez Pila ◽  
A. Grohmann
Keyword(s):  
Escherichia Coli ◽  
Legionella Pneumophila ◽  
Stopped Flow ◽  
Standard Laboratory ◽  
Human Pathogens ◽  
Laboratory Equipment ◽  
Simple Method ◽  
Flow Apparatus ◽  
Pool Area ◽  
Good Agreement

Prevention of infectious diseases transmission in swimming and bathing pools requires that sufficient disinfection capability is constantly maintained in the bathing water. Accordingly, German regulations make mandatory that any procedure for the treatment and disinfection of those waters must guarantee a certain minimum capability to inactivate human pathogens in the water. We describe here a simple method to evaluate the disinfection capability of pool water, expressed as the inactivation rate of selected microorganisms (Pseudomonas aeruginosa,Escherichia coli and Legionella pneumophila). The test might be carried out right onsite in the pool area using simple standard laboratory equipment normally present in microbiological laboratories and might be used for the evaluation of standard disinfectants and of others to be developed in the future. Results achieved with this test show good agreement with those obtained using a sophisticated stopped flow apparatus, developed exclusively for the evaluation of disinfection capability.

scholarly journals Enrichment of Pachytene Spermatocytes and Spermatids from Mouse Testes Using Standard Laboratory Equipment

10.3791/60271 ◽  
2019 ◽  
Author(s):  
Matteo Da Ros ◽  
Tiina Lehtiniemi ◽  
Opeyemi Olotu ◽  
Oliver Meikar ◽  
Noora Kotaja
Keyword(s):  
Standard Laboratory ◽  
Laboratory Equipment ◽  
Pachytene Spermatocytes

scholarly journals Application of retrotransposon-based Inter-SINE Amplified Polymorphism (ISAP) markers for the differentiation of common poplar genotypes

2021 ◽  
Author(s):  
Birgit Reiche ◽  
Anja Koegler ◽  
Kristin Morgenstern ◽  
Marie Brueckner ◽  
Beatrice Weber ◽  
...  
Keyword(s):  
Simple Sequence Repeat ◽  
Genetic Background ◽  
Plant Material ◽  
Standard Laboratory ◽  
Chain Reaction ◽  
Laboratory Equipment ◽  
High Identity ◽  
Poplar Trees ◽  
Polymerase Chain ◽  
Simple Sequence

On the basis of retrotransposon-insertion polymorphisms, molecular markers were developed for the identification and differentiation of poplar (<i>Populus</i> spp.) genotypes. For this purpose, short interspersed nuclear elements (SINEs) in the genome sequence of <i>Populus tremula</i> were identified and assigned to different SINE-families. For families with high copy number and high identity values, primers were derived to amplify Inter- SINE Amplified Polymorphisms (ISAPs) with polymerase chain reaction (PCR). The resulting fragments produce genotype-specific fingerprints. This molecular approach utilizes standard laboratory equipment and is therefore easy to use for the verification of plant material. We demonstrate the functionality of three distinct ISAP primer combinations by comparison to ten simple sequence repeat (SSR) markers to differentiate 23 poplar genotypes. Already by using a single ISAP primer combination all genotypes can be clearly discriminated. Furthermore, the cluster analysis based on three primer combinations divides clones according to their genetic background into two subclusters (by a bootstrap value of 98). Our results clearly demonstrate the usability of ISAP markers to differentiate genotypes and trace progenies of poplar trees.

scholarly journals Asymmetric depth-filtration - a versatile and scalable approach for isolation and purification of extracellular vesicles

2021 ◽  
Author(s):  
Vasiliy S. Chernyshev ◽  
Roman N. Chuprov-Netochin ◽  
Ekaterina Tsydenzhapova ◽  
Elena Victorovna Svirshchevskaya ◽  
Rimma A. Poltavtseva ◽  
...  
Keyword(s):  
Extracellular Vesicles ◽  
Biological Samples ◽  
Biological Fluids ◽  
Scale Up ◽  
Size Exclusion ◽  
Standard Laboratory ◽  
Laboratory Equipment ◽  
Isolation And Purification ◽  
Depth Filtration ◽  
Exclusion Chromatography

A novel asymmetric depth filtration (DF) approach for isolation of extracellular vesicles (EVs) from biological fluids is presented, and its performance is compared with established methods. The developed workflow is simple, inexpensive, and relatively fast. Compared with ultracentrifugation and size-exclusion chromatography, the developed method isolates EVs with higher purity and yield. Only standard laboratory equipment is needed for its implementation, which makes it suitable for low-resource locations. The described implementation of the method is suitable for EV isolation from small biological samples in diagnostic and treatment guidance applications. Following the scale-up routes adopted in the biomanufacturing of therapeutics, which routinely rely on DF as one of the product purification steps, the developed method may be scaled up to harvesting therapeutic EVs from large volumes of growth medium.

scholarly journals Use of the water-swellable polymers (WSP) for wellbore stabilization in intensely fractured rock intervals

2021 ◽  
Vol 266 ◽  
pp. 01013
Author(s):  
M.I. Sadykov ◽  
P.A. Blinov ◽  
M.V. Nutskova
Keyword(s):  
Fractured Rocks ◽  
Fractured Rock ◽  
Drilling Fluid ◽  
Standard Laboratory ◽  
Laboratory Equipment ◽  
Stability Coefficient ◽  
Complex Effect ◽  
Fluid Properties ◽  
The Stability ◽  
The One

Current research on the stability of well walls when drilling in fractured rocks and also when drilling inclined sections of the well profile are based on the assessment of the effect of drilling fluid filtrate. The novelty of this work lies in studying the complex effect of a water-swellable polymer on the stability of well wall, on the one hand, and on the controlled loss of circulation on the other. For preparing rock samples, a method was used based on standard laboratory equipment fordrilling fluid properties. The well wall stability tests are performedon a direct shear apparatus.The WSP composition based on alcohol, ether, and surfactant (Polyecanol Flora) showed its efficiency by increasing the stability coefficient with the engagement of less and more internal friction angle.PHPA-based viscoelastic composition showed the best result by increasing the stability coefficient in the hazardous areas by 50-60%.

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