Methods for Establishing and Using a Stable Cell Line Expressing Both Gaussia Luciferase and Firefly Luciferase to Screen for Endoplasmic Reticulum Stress

2020 ◽  
pp. 531-555
Author(s):  
Zhen Ren ◽  
Si Chen ◽  
Lei Guo
Keyword(s):  
Endoplasmic Reticulum ◽  
Endoplasmic Reticulum Stress ◽  
Cell Line ◽  
Firefly Luciferase ◽  
Stable Cell Line ◽  
Gaussia Luciferase

scholarly journals Propofol elicits autophagy via endoplasmic reticulum stress and calcium exchange in C2C12 myoblast cell line

PLoS ONE ◽  
2018 ◽  
Vol 13 (5) ◽  
pp. e0197934 ◽  
Author(s):  
Xi Chen ◽  
Long-Yun Li ◽  
Jin-Lan Jiang ◽  
Kai Li ◽  
Zhen-Bo Su ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Endoplasmic Reticulum Stress ◽  
Cell Line ◽  
C2c12 Myoblast ◽  
Myoblast Cell Line ◽  
Calcium Exchange ◽  
Myoblast Cell

scholarly journals Induction of Apoptosis in Human Pancreatic Cancer Stem Cells by the Endoplasmic Reticulum-Targeted Alkylphospholipid Analog Edelfosine and Potentiation by Autophagy Inhibition

Cancers ◽  
2021 ◽  
Vol 13 (23) ◽  
pp. 6124
Author(s):  
Consuelo Gajate ◽  
Odile Gayet ◽  
Nicolas A. Fraunhoffer ◽  
Juan Iovanna ◽  
Nelson Dusetti ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Stem Cells ◽  
Endoplasmic Reticulum ◽  
Cancer Stem Cells ◽  
Endoplasmic Reticulum Stress ◽  
Cell Line ◽  
Cancer Patients ◽  
Caspase 3 ◽  
Human Fibroblasts ◽  
Human Pancreatic Cancer

Pancreatic cancer is one of the most lethal malignancies with a poor and gloomy prognosis and the highest mortality-to-incidence ratio. Pancreatic cancer remains an incurable malignancy, and current therapies are ineffective. We isolated cancer stem cells (CSCs) from the human PANC-1 pancreatic cancer cell line as CD44+CD24+EpCAM+ cells. These CSCs form pancreatic cancer spheres or spheroids and develop tumors in SCID mice after subcutaneous injection of as few as 100 cells per mouse. Here, we found that the alkylphospholipid analog edelfosine inhibited CSC pancreatic cancer spheroid formation and induced cell death, as assessed by an increase in the percentage of cells in the sub-G0/G1 region by means of flow cytometry, indicative of DNA breakdown and apoptosis. This correlated with an increase in caspase-3 activity and PARP breakdown, as a major substrate of caspase-3, following PANC-1 CSC treatment with edelfosine. The antitumor ether lipid edelfosine colocalized with the endoplasmic reticulum in both PANC-1 cells as well as PANC-1 CSCs by using a fluorescent edelfosine analog, and induced an endoplasmic reticulum stress response in both PANC-1 cells and PANC-1 CSCs, with a potent CHOP/GADD153 upregulation. Edelfosine elicited a strong autophagy response in both PANC-1 cells and PANC-1 CSCs, and preincubation of CSCs with autophagy inhibitors, chloroquine or bafilomycin A1, enhanced edelfosine-induced apoptosis. Primary cultures from pancreatic cancer patients were sensitive to edelfosine, as well as their respective isolated CSCs. Nontumorigenic pancreatic human cell line HPNE and normal human fibroblasts were largely spared. These data suggest that pancreatic CSCs isolated from established cell lines and pancreatic cancer patients are sensitive to edelfosine through its accumulation in the endoplasmic reticulum and induction of endoplasmic reticulum stress.

285 HCV expression in a tetracycline-regulated cell line activates endoplasmic reticulum stress-mediated apoptosis

2004 ◽  
Vol 40 ◽  
pp. 88
Author(s):  
A.R. Ciccaglione ◽  
A. Costantino ◽  
E. Tritarelli ◽  
C. Marcantonio ◽  
M. Equestre ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Endoplasmic Reticulum Stress ◽  
Cell Line
Sign in / Sign up

Export Citation Format

Share Document