Detection of Blood Parasites and Estimation of Hematological Indices in Fish

The influence of vitamin E and trolox on biochemical and hematological indices in turkey hen's blood

Annales UMCS Zootechnica ◽

10.2478/v10083-010-0007-9 ◽

2010 ◽

Vol 28 (2) ◽

Author(s):

Katarzyna Rostek

Keyword(s):

Vitamin E ◽

Hematological Indices

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Identification of Blood Parasites in Individuals from Six Families of Freshwater Turtles

Chelonian Conservation and Biology ◽

10.2744/ccb-1411.1 ◽

2020 ◽

Vol 19 (1) ◽

pp. 85

Author(s):

Stephanie C. Nordmeyer ◽

Gina Henry ◽

Trina Guerra ◽

David Rodriguez ◽

Michael R.J. Forstner ◽

...

Keyword(s):

Blood Parasites ◽

Freshwater Turtles

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Prevalence and genetic diversity of avian haemosporidian parasites in wild bird species of the order Columbiformes

Parasitology Research ◽

10.1007/s00436-021-07053-7 ◽

2021 ◽

Author(s):

Yvonne R. Schumm ◽

Dimitris Bakaloudis ◽

Christos Barboutis ◽

Jacopo G. Cecere ◽

Cyril Eraud ◽

...

Keyword(s):

Host Species ◽

Large Scale ◽

Phylogenetic Reconstruction ◽

Bird Species ◽

Avian Species ◽

Blood Parasites ◽

Migration Strategy ◽

Haemosporidian Parasites ◽

Multiplex Pcr Assay ◽

And Migration

AbstractDiseases can play a role in species decline. Among them, haemosporidian parasites, vector-transmitted protozoan parasites, are known to constitute a risk for different avian species. However, the magnitude of haemosporidian infection in wild columbiform birds, including strongly decreasing European turtle doves, is largely unknown. We examined the prevalence and diversity of haemosporidian parasites Plasmodium, Leucocytozoon and subgenera Haemoproteus and Parahaemoproteus in six species of the order Columbiformes during breeding season and migration by applying nested PCR, one-step multiplex PCR assay and microscopy. We detected infections in 109 of the 259 screened individuals (42%), including 15 distinct haemosporidian mitochondrial cytochrome b lineages, representing five H. (Haemoproteus), two H. (Parahaemoproteus), five Leucocytozoon and three Plasmodium lineages. Five of these lineages have never been described before. We discriminated between single and mixed infections and determined host species-specific prevalence for each parasite genus. Observed differences among sampled host species are discussed with reference to behavioural characteristics, including nesting and migration strategy. Our results support previous suggestions that migratory birds have a higher prevalence and diversity of blood parasites than resident or short-distance migratory species. A phylogenetic reconstruction provided evidence for H. (Haemoproteus) as well as H. (Parahaemoproteus) infections in columbiform birds. Based on microscopic examination, we quantified parasitemia, indicating the probability of negative effects on the host. This study provides a large-scale baseline description of haemosporidian infections of wild birds belonging to the order Columbiformes sampled in the northern hemisphere. The results enable the monitoring of future changes in parasite transmission areas, distribution and diversity associated with global change, posing a potential risk for declining avian species as the European turtle dove.

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Correlation of non-clinical parameters with the hematological indices in type 2 Diabetic Mellitus patients

Heliyon ◽

10.1016/j.heliyon.2021.e07366 ◽

2021 ◽

pp. e07366

Author(s):

Vamitha Paneerselvam Sampathkumar ◽

Prathipa Krishnamurthy ◽

Saravanan Balaraman ◽

Dhivya Balaiya ◽

Ravi Sivaraman ◽

...

Keyword(s):

Clinical Parameters ◽

Diabetic Mellitus ◽

Hematological Indices ◽

Type 2 Diabetic Mellitus ◽

Type 2 Diabetic

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Blood parasites of bird communities in Sri Lanka and their mosquito vectors

Parasitology Research ◽

10.1007/s00436-021-07049-3 ◽

2021 ◽

Vol 120 (2) ◽

pp. 693-703

Author(s):

W. G. D. Chathuranga ◽

B. R. Fernando ◽

T. C. Weereratne ◽

S. H. P. P. Karunaratne ◽

W. A. Priyanka P. De Silva

Keyword(s):

Sri Lanka ◽

Bird Communities ◽

Blood Parasites ◽

Mosquito Vectors

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Lankesterella (Apicomplexa, Lankesterellidae) Blood Parasites of Passeriform Birds: Prevalence, Molecular and Morphological Characterization, with Notes on Sporozoite Persistence In Vivo and Development In Vitro

Animals ◽

10.3390/ani11051451 ◽

2021 ◽

Vol 11 (5) ◽

pp. 1451

Author(s):

Carolina Romeiro Fernandes Chagas ◽

Josef Harl ◽

Vytautas Preikša ◽

Dovilė Bukauskaitė ◽

Mikas Ilgūnas ◽

...

Keyword(s):

Morphological Characterization ◽

Life Cycles ◽

Diagnostic Methods ◽

Host Cells ◽

Blood Parasites ◽

Serinus Canaria ◽

Long Time ◽

Development In Vitro

Recent studies confirmed that some Hepatozoon-like blood parasites (Apicomplexa) of birds are closely related to the amphibian parasite Lankesterella minima. Little is known about the biology of these pathogens in birds, including their distribution, life cycles, specificity, vectors, and molecular characterization. Using blood samples of 641 birds from 16 species, we (i) determined the prevalence and molecular diversity of Lankesterella parasites in naturally infected birds; (ii) investigated the development of Lankesterella kabeeni in laboratory-reared mosquitoes, Culex pipiens forma molestus and Aedes aegypti; and (iii) tested experimentally the susceptibility of domestic canaries, Serinus canaria, to this parasite. This study combined molecular and morphological diagnostic methods and determined 11% prevalence of Lankesterella parasites in Acrocephalidae birds; 16 Lankesterella lineages with a certain degree of host specificity and two new species (Lankesterella vacuolata n. sp. and Lankesterella macrovacuolata n. sp.) were found and characterized. Lankesterella kabeeni (formerly Hepatozoon kabeeni) was re-described. Serinus canaria were resistant after various experimental exposures. Lankesterella sporozoites rapidly escaped from host cells in vitro. Sporozoites persisted for a long time in infected mosquitoes (up to 42 days post exposure). Our study demonstrated a high diversity of Lankesterella parasites in birds, and showed that several avian Hepatozoon-like parasites, in fact, belong to Lankesterella genus.

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Invasive lizard has fewer parasites than native congener

Parasitology Research ◽

10.1007/s00436-021-07233-5 ◽

2021 ◽

Author(s):

Beatriz Tomé ◽

D. James Harris ◽

Ana Perera ◽

Isabel Damas-Moreira

Keyword(s):

18S Rrna ◽

Blood Parasites ◽

Average Intensity ◽

Invasive Potential ◽

Lizard Species ◽

High Prevalence ◽

Podarcis Siculus ◽

Species Establishment ◽

Low Levels ◽

Italian Wall Lizard

AbstractInvasive species can carry parasites to introduced locations, which may be key to understand the success or failure of species establishment and the invasive potential of introduced species. We compared the prevalence and infection levels of haemogregarine blood parasites between two sympatric congeneric species in Lisbon, Portugal: the invasive Italian wall lizard (Podarcis siculus) and the native green Iberian wall lizard (Podarcis virescens). The two species had significant differences in their infection levels: while P. virescens had high prevalence of infection (69.0%), only one individual of P. siculus was infected (3.7%), and while P. virescens exhibited an average intensity of 1.36%, the infected P. siculus individual had an infection rate of only 0.04%. Genetic analyses of 18S rRNA identified two different haemogregarine haplotypes in P. virescens. Due to the low levels of infection, we were not able to amplify parasite DNA from the infected P. siculus individual, although it was morphologically similar to those found in P. virescens. Since other studies also reported low levels of parasites in P. siculus, we hypothesize that this general lack of parasites could be one of the factors contributing to its competitive advantage over native lizard species and introduction success.

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