Vietnamese as Potential Resources to Explore New Anticancer and : Established Assays for Pharmacological Tests

2021 ◽  
pp. 271-286
Author(s):  
Trang Thi Phuong Nguyen ◽  
Dieu Thi Xuan Nguyen ◽  
Triet Thanh Nguyen
Keyword(s):  
Pharmacological Tests

Some 2-substitution derivatives of 5-(4-oxo-6-hydroxy-3,4-dihydro-5-pyrimidinyl)pentanoic acid

1983 ◽  
Vol 48 (1) ◽  
pp. 304-311 ◽  
Author(s):  
Jiří Křepelka ◽  
Jan Beneš ◽  
Vladimír Pouzar ◽  
Jaroslav Vachek ◽  
Jiří Holubek
Keyword(s):  
Nitrile Group ◽  
Antineoplastic Activity ◽  
Carboxyl Group ◽  
Positional Isomers ◽  
Pentanoic Acid ◽  
Pharmacological Tests ◽  
Derivatives Of

Condensation of triethyl ester of 1,1,5-pentanetricarboxylic acid (XI) with substituted guanidines XXII - XXIX gave acids II - IX, which were converted into esters XI - XIX. The acid II and the ester XI were obtained as mixtures of positional isomers. Analogously, condensation of the triester XXI with dicyanodiamide gave rise to acid X, whose nitrile group, under conditions of esterification of a carboxyl group, produced iminoether XX. In pharmacological tests for antineoplastic activity the compounds prepared exhibited weaker efficacy than 5-(2-amino-6-hydroxy-4-oxo-3,4-dihydro-5-pyrimidinyl)pentanoic acid (I), employed as standard.

Pharmacological Tests

1974 ◽  
pp. 266-269
Author(s):  
J. Janssens ◽  
J. Hellemans
Keyword(s):  
Pharmacological Tests

Intrinsic Properties Shape the Firing Pattern of Ventral Horn Interneurons From the Spinal Cord of the Adult Turtle

2006 ◽  
Vol 96 (5) ◽  
pp. 2670-2677 ◽  
Author(s):  
Morten Smith ◽  
Jean-François Perrier
Keyword(s):  
Spinal Cord ◽  
Activity Patterns ◽  
Ventral Horn ◽  
Inward Rectification ◽  
Intrinsic Properties ◽  
Patch Clamp Technique ◽  
Firing Patterns ◽  
Pharmacological Tests ◽  
Low Threshold

Interneurons in the ventral horn of the spinal cord play a central role in motor control. In adult vertebrates, their intrinsic properties are poorly described because of the lack of in vitro preparations from the spinal cord of mature mammals. Taking advantage of the high resistance to anoxia in the adult turtle, we used a slice preparation from the spinal cord. We used the whole cell blind patch-clamp technique to record from ventral horn interneurons. We characterized their firing patterns in response to depolarizing current pulses and found that all the interneurons fired repetitively. They displayed bursting, adapting, delayed, accelerating, or oscillating firing patterns. By combining electrophysiological and pharmacological tests, we showed that interneurons expressed slow inward rectification, plateau potential, voltage-sensitive transient outward rectification, and low-threshold spikes. These results demonstrate a diversity of intrinsic properties that may enable a rich repertoire of activity patterns in the network of ventral horn interneurons.

scholarly journals Molecular and functional characterization of somatostatin-type signalling in a deuterostome invertebrate

Open Biology ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 200172
Author(s):  
Ya Zhang ◽  
Luis Alfonso Yañez Guerra ◽  
Michaela Egertová ◽  
Cleidiane G. Zampronio ◽  
Alexandra M. Jones ◽  
...  
Keyword(s):  
Functional Characterization ◽  
The Body ◽  
Pharmacological Characterization ◽  
Cardiac Stomach ◽  
Physiological Processes ◽  
Pharmacological Tests ◽  
The Central Nervous System

Somatostatin (SS) and allatostatin-C (ASTC) are structurally and evolutionarily related neuropeptides that act as inhibitory regulators of physiological processes in mammals and insects, respectively. Here, we report the first molecular and functional characterization of SS/ASTC-type signalling in a deuterostome invertebrate—the starfish Asterias rubens (phylum Echinodermata). Two SS/ASTC-type precursors were identified in A. rubens (ArSSP1 and ArSSP2) and the structures of neuropeptides derived from these proteins (ArSS1 and ArSS2) were analysed using mass spectrometry. Pharmacological characterization of three cloned A. rubens SS/ASTC-type receptors (ArSSR1–3) revealed that ArSS2, but not ArSS1, acts as a ligand for all three receptors. Analysis of ArSS2 expression in A. rubens using mRNA in situ hybridization and immunohistochemistry revealed stained cells/fibres in the central nervous system, the digestive system (e.g. cardiac stomach) and the body wall and its appendages (e.g. tube feet). Furthermore, in vitro pharmacological tests revealed that ArSS2 causes dose-dependent relaxation of tube foot and cardiac stomach preparations, while injection of ArSS2 in vivo causes partial eversion of the cardiac stomach. Our findings provide new insights into the molecular evolution of SS/ASTC-type signalling in the animal kingdom and reveal an ancient role of SS-type neuropeptides as inhibitory regulators of muscle contractility.

Autonomic Stimulation, Osmolarity and Prostaglandin Effects in the Eustachian Tube

1976 ◽  
Vol 85 (2_suppl) ◽  
pp. 187-193 ◽  
Author(s):  
Richard T. Jackson
Keyword(s):  
Smooth Muscle ◽  
Eustachian Tube ◽  
Middle Ear ◽  
Sphenopalatine Ganglion ◽  
Greater Petrosal Nerve ◽  
Pharmacological Tests ◽  
Radioimmune Assay ◽  
Human Middle Ear ◽  
Pooled Samples ◽  
Stimulation Of

Stimulation of the vidian nerve in the dog induced an apparent vasodilation in the mucosa of the Eustachian tube. It is presumed that these parasympathetic fibers course from the greater petrosal nerve through the vidian to the sphenopalatine ganglion. The pharyngeal nerve arises from the ganglion and innervates the mucosa of the tube. Perfusion of the dog's Eustachian tube with solutions of various osmolarities caused a shrinking or swelling of the tube mucosa. Ten percent alterations in the concentration of saline, Ringer's or isotonic KCl produced a hypo- or hypertonic effect. Systemic administration of osmotic diuretics was shown to remove water from normal and edematous tubal mucosa. An agent in human middle ear effusions was found to contract smooth muscle. The behavior of this agent in pharmacological tests suggested the presence of prostaglandins (PG). The effusions were tested for the presence of other inflammatory mediators such as bradykinin, acetylcholine and histamine. These were felt to be absent or present in small amounts. A radioimmune assay of pooled samples of middle ear effusions revealed the presence of several PG, notably PGF2α and PGE2. There appeared to be a higher concentration of PG in mucoid effusions than serous effusions. The inflammatory capabilities of these agents are mentioned.

Effect of Chinese Herbal Medicine „Hange-Koboku-To” on Laryngeal Reflex of Cats and in other Pharmacological Tests

Planta Medica ◽  
1983 ◽  
Vol 47 (01) ◽  
pp. 59-62 ◽  
Author(s):  
Aiko Sugaya ◽  
Tadashi Tsuda ◽  
Tadashi Obuchi ◽  
Eiichi Sugaya
Keyword(s):  
Herbal Medicine ◽  
Chinese Herbal Medicine ◽  
Chinese Herbal ◽  
Pharmacological Tests

scholarly journals Efficiently Capturing Mitochondria-Targeted Constituents with Hepatoprotective Activity from Medicinal Herbs

2019 ◽  
Vol 2019 ◽  
pp. 1-18 ◽  
Author(s):  
Xing-Xin Yang ◽  
Li Liang ◽  
Xin Liu ◽  
Feng-Jiao Li ◽  
Jin-Cai Dong ◽  
...  
Keyword(s):  
Energy Production ◽  
Hepatoprotective Activity ◽  
Medicinal Herbs ◽  
Oxygen Species ◽  
Liquid Chromatography Mass Spectrometry ◽  
Pharmacological Tests ◽  
Protective Strategy ◽  
Mitochondria Targeting ◽  
L02 Cells

Targeting mitochondria as a hepatic-protective strategy has gained attention, because of their important roles in energy production, adjustment of apoptosis, and generation of reactive oxygen species. To promote the discovery of natural mitochondria-targeted hepatic-protectants, we established a hepatocellular mitochondria-based capturing method by coupling affinity ultrafiltration with liquid chromatography/mass spectrometry (LC/MS), which is suitable for identifying mitochondrial ligands from medicinal herbs (MHs). After evaluating the feasibility of the method, it was applied for capturing mitochondria-targeting constituents from Peucedani Radix extract. A total of 10 active compounds were identified by LC/MS, all of which were newly identified mitochondrial ligands. The mitochondria-remedying activity of 4 of the 10 hits was confirmed by pharmacological tests in vitro. Additionally, the hepatic-protective abilities of 4 hits were verified in both carbon tetrachloride-damaged liver L02 cells and mice. These results indicated that the method could be used for identifying hepatic mitochondria-targeting constituents in MHs, which might be beneficial for hepatic-protective development.

Versuche, sedativ-hypnotische Wirkstoffe im Hopfen nachzuweisen, II / Narcotic Action of 2-Methyl-3-butene-2-ol Contained in the Exhalation of Hops

1980 ◽  
Vol 35 (11-12) ◽  
pp. 1096-1097 ◽  
Author(s):  
Rudolf Hänsel ◽  
Rainer Wohlfart ◽  
Helmut Coper
Keyword(s):  
Essential Oil ◽  
Water Solubility ◽  
Volatile Fraction ◽  
Abnormal Behaviour ◽  
Pharmacological Tests ◽  
Narcotic Action

Hops are told to promote sleep; manyfold efforts to detect the soporific principle have been unsuccessful so far. Preliminary pharmacological tests lead to the con­clusion that the soporific activity of the exhalation of hops can be explained by its content of 2-methyl-3-butene-2-ol (1) in the volatile fraction. It was found that 1, when given to mices i.p. (0.80 g/kg) produces narcosis for about 8 h; no abnormal behaviour was observed there upon. Due to its water-solubility the concentration of 1 in the essential oil obtained by steam-destination is low; contrary to that, 1, is enriched in the more volatile fraction of hops.

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