scholarly journals Empirical Validation of Overlapping Virus lncRNAs and Coding Transcripts by Northern Blot

Author(s):  
Mehmet Kara ◽  
Scott A. Tibbetts
2007 ◽  
Vol 148 (23) ◽  
pp. 1067-1075
Author(s):  
Krisztina Fischer ◽  
Orsolya Galamb ◽  
Béla Molnár ◽  
Zsolt Tulassay ◽  
András Szabó

A gyermekkori nephrosis 90%-a idiopathiás nephrosis szindróma. Az idetartozó három kórkép, a minimal change betegség, a mesangialis proliferatio és a focalis sclerosis hasonló klinikai képpel jelentkező, eltérő prognózisú és terápiás válaszú betegség. Dolgozatunk célja az idiopathiás nephrosis szindrómába tartozó kórképek kialakulásával, progressziójával összefüggő genetikai ismeretek, génexpressziós változások áttekintése és funkcionális csoportosítása. A génexpressziós változások meghatározásának eszközeként, dolgozatunk röviden összefoglalja a northern blot, a ribonuclease protection assay, az in situ RNS-hibridizáció, a kvantitatív RT-PCR és a microarray módszerek lényegét. Az eddig elvégzett vizsgálatok a DNS-szintézis és repair gének, növekedési faktorok, extracelluláris mátrix, extracelluláris ligandreceptorok, extracelluláris jelátvitel zavarai mellett kiemelik a metabolikus és transzporter gének, illetve az immunszabályozó gének molekuláris eltéréseit, amelyek összefüggésben vannak az idiopathiás nephrosis szindróma eddig megismert molekuláris hátterével. A chiptechnológia fejlődésével és elterjedésével ezek a markerek és a hagyományos vizsgálati módszerek párhuzamos alkalmazása rutindiagnosztikai szempontból is fontossá válhat.


2017 ◽  
Author(s):  
Eugenia Isabel Gorlin ◽  
Michael W. Otto

To live well in the present, we take direction from the past. Yet, individuals may engage in a variety of behaviors that distort their past and current circumstances, reducing the likelihood of adaptive problem solving and decision making. In this article, we attend to self-deception as one such class of behaviors. Drawing upon research showing both the maladaptive consequences and self-perpetuating nature of self-deception, we propose that self-deception is an understudied risk and maintaining factor for psychopathology, and we introduce a “cognitive-integrity”-based approach that may hold promise for increasing the reach and effectiveness of our existing therapeutic interventions. Pending empirical validation of this theoretically-informed approach, we posit that patients may become more informed and autonomous agents in their own therapeutic growth by becoming more honest with themselves.


BIO-PROTOCOL ◽  
2013 ◽  
Vol 3 (7) ◽  
Author(s):  
Yuehua Wei
Keyword(s):  

Author(s):  
Kim Fridkin ◽  
Patrick Kenney

This book develops and tests the “tolerance and tactics theory of negativity.” The theory argues that citizens differ in their tolerance of negative campaigning. Also, candidates vary in the tactics used to attack their opponents, with negative messages varying in their relevance to voters and in the civility of their tone. The interplay between citizens’ tolerance of negativity and candidates’ negative messages helps clarify when negative campaigning will influence citizens’ evaluations of candidates and their likelihood of voting. A diverse set of data sources was collected from U.S. Senate elections (e.g., survey data, experiments, content analysis, focus groups) across several years to test the theory. The tolerance and tactics theory of negativity receives strong empirical validation. First, people differ systematically in their tolerance for negativity, and their tolerance changes over the course of the campaign. Second, people’s levels of tolerance consistently and powerfully influence how they assess negative messages. Third, the relevance and civility of negative messages consistently influence citizens’ assessments of candidates competing for office. That is, negative messages focusing on relevant topics and utilizing an uncivil tone produce significant changes in people’s impressions of the candidates. Furthermore, people’s tolerance of negativity influences their susceptibility to negative campaigning. Specifically, relevant and uncivil messages are most influential for people who are least tolerant of negative campaigning. The relevance and civility of campaign messages also alter people’s likelihood of voting, and the impact of negative messages on turnout is more consequential for people with less tolerance of negativity.


Smart Cities ◽  
2021 ◽  
Vol 4 (1) ◽  
pp. 195-203
Author(s):  
Eric Garrison ◽  
Joshua New

While urban-scale building energy modeling is becoming increasingly common, it currently lacks standards, guidelines, or empirical validation against measured data. Empirical validation necessary to enable best practices is becoming increasingly tractable. The growing prevalence of advanced metering infrastructure has led to significant data regarding the energy consumption within individual buildings, but is something utilities and countries are still struggling to analyze and use wisely. In partnership with the Electric Power Board of Chattanooga, Tennessee, a crude OpenStudio/EnergyPlus model of over 178,000 buildings has been created and used to compare simulated energy against actual, 15-min, whole-building electrical consumption of each building. In this study, classifying building type is treated as a use case for quantifying performance associated with smart meter data. This article attempts to provide guidance for working with advanced metering infrastructure for buildings related to: quality control, pathological data classifications, statistical metrics on performance, a methodology for classifying building types, and assess accuracy. Advanced metering infrastructure was used to collect whole-building electricity consumption for 178,333 buildings, define equations for common data issues (missing values, zeros, and spiking), propose a new method for assigning building type, and empirically validate gaps between real buildings and existing prototypes using industry-standard accuracy metrics.


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