Genetic Diversity Among Pseudomonad Strains Associated with Cereal Diseases in Russian Federation

The paper presents the results of a study of the D-loop area in Landrace pigs (n=49). Amplification of the mtDNA D-loop fragment was performed according to Sanger's method. The experiments showed that modern Landrace pigs in the breeding farms of the Russian Federation are distinguished by a sufficient intra-breed diversity and have different haplotypes that belong to both the European and Asian haplogroups.

Download Full-text

NG-MAST GENOTYPING OF NEISSERIA GONORRHOEAE STRAINS ISOLATED IN RUSSIAN FEDERATION IN 2012 - 2015

Journal of microbiology epidemiology immunobiology ◽

10.36233/0372-9311-2016-4-42-51 ◽

2016 ◽

pp. 42-51 ◽

Cited By ~ 2

Author(s):

D. V. Vorobiev ◽

V. S. Solomka ◽

K. I. Plakhova ◽

D. G. Deryabin ◽

A. A. Kubanov

Keyword(s):

Genetic Diversity ◽

Neisseria Gonorrhoeae ◽

Russian Federation ◽

Variable Regions ◽

The Russian Federation ◽

First Time ◽

Sequence Types

Aim. Characteristics of contemporary strains of N. gonorrhoeae using NG-MAST genotyping (.Neisseria gonorrhoeae multi-antigen sequence typing), isolation of dominating variants and analysis of their territorial distribution. Materials and methods. 440 strains of N. gonorrhoeae isolated in 2012 - 2015 in 19 subjects of the Russian Federation were used in the study. Genotyping was carried out based on sequencing of variable regions of porB and tbpB genes. Identification of alleles and sequence-types was carried out according to http://www.ng-mast.net. Results. 172 NG-MAST genotypes were identified, 100 of which -were described for the first time. 807,1152, 1544,5714 and 5941 were predominating sequence-types, typical for several subjects of the Russian Federation and some neighboring countries, as well as sequence-types 8583 and 9476 described for the first time, that were exclusively distributed in the regions of their emergence. Sequence-types 225, 1407 and 2992, that are epidemically significant in distant countries, were represented by single isolates in this study. Conclusion. A peculiar and significant genetic diversity of N. gonorrhoeae strains, circulating in the Russian Federation, with characteristic bond of several NG-MAST genotypes to one or several regions, was shown.

Download Full-text

GENETIC DIVERSITY OF HEPATITIS C VIRUS IN NANAIAN REGION, KHABAROVSK TERRITORY

Russian Journal of Infection and Immunity ◽

10.15789/2220-7619-gdo-1265 ◽

2019 ◽

Author(s):

V. Kotova ◽

L. Balakhontseva ◽

E. Bazykina ◽

O. Trotsenko ◽

V. Beldy ◽

...

Keyword(s):

Genetic Diversity ◽

Chronic Hepatitis ◽

Hepatitis C Virus ◽

Hepatitis C ◽

Chronic Hepatitis C ◽

Russian Federation ◽

Hcv Rna ◽

Central Research ◽

Plasma Samples ◽

Genotype 2

Examining hepatitis C virus (HCV) genetic diversity is of great practical value in molecular-epidemiological research, development of specific prevention tools and outlining therapeutic strategy. Aim of study: to conduct analysis assessing HCV genetic diversity circulating in the Nanaisky region population of the Khabarovsk territory. Materials and Methods. Molecular and genetic analysis of 124 blood plasma samples collected from patients with chronic hepatitis C and residing in the Nanaisky region was conducted. Results. HCV RNA was detected in 84 (67.7±4.2%) plasma samples. HCV genotyping was performed by using AmpliSens – 1/2/3 kit (Central Research Institute of Epidemiology, Moscow, Russian Federation) showing that genotype 3 dominated reaching up to 47.6±5.4% (n=40). Genotype 1 was detected in 30 patients (35.7±5.2%). In thirteen cases (15.5±3.9%) genotype 2 was identified, whereas in one case (1.2±1.2%) virus genotype was unidentified. Phylogenetic analysis of the nucleotide sequences in HCV NS5B region was performed for 60 HCV RNA-positive samples showing subtype ratio as follows: 1a - 2 (3.3±2.3%), 1b - 23 (38.3±6.3%), 2а – 6 (10.0±3.9%), 2с – 2 (3.3±2.3%), 3а – 27(45.0±6.4%). Three samples of RF2k/1b recombinant virus were found. A full NS2 gene nucleotide sequence was cloned in order to confirm the recombination event. The results of the study evidence about a need to conduct multi-layered examination of patients with chronic hepatitis C by using current molecular and biologic methods for assigning proper therapy coupled to characteristics of the isolated strains. The data regarding hepatitis C virus molecular and genetic parameters circulating in the Far Eastern Federal District, Russia, are rather limited. Hence, our study would contribute to current understanding of HCV genovariants circulating in territories of the Russian Federation.

Download Full-text

Analysis of the genetic diversity of Russian common oat varieties using alleles of avenin-coding loci

BIO Web of Conferences ◽

10.1051/bioconf/20213601015 ◽

2021 ◽

Vol 36 ◽

pp. 01015

Author(s):

A.V. Lyubimova ◽

D.I. Eremin ◽

I.G. Loskutov ◽

S.A. Levshtanov ◽

I.B. Trifuntova

Keyword(s):

Genetic Diversity ◽

Frequency Distribution ◽

Russian Federation ◽

Genetic Erosion ◽

Population Diversity ◽

Frequency Of Occurrence ◽

Breeding Programs ◽

Irkutsk Region ◽

The Russian Federation ◽

The Stability

Alleles of avenin-coding loci were identified in 24 varieties of common oat origin from 6 different breeding centers of the Russian Federation. It was found that 33% of the studied varieties are homogeneous. Heterogeneous varieties contain from 2 to 10 biotypes. The analysis revealed 67 different genotypes, ten of which are found in the spectra of several varieties at once. Despite the presence of matching genotypes, all the studied varieties differ in the genetic formulas of avenin, the number and frequency of occurrence of biotypes. This makes it possible to effectively identify and distinguish oat varieties sown by Russian breeding using the method of prolamin electrophoresis. The analyzed groups of varieties are characterized by high values of genetic (0.54-0.79) and intra-population diversity, which indicates the stability of populations. The exception is varieties originating from the Irkutsk region, low values of genetic and intra-population diversity in the population of which may indicate the process of genetic erosion. Analysis of the frequency distribution of alleles of avenin-coding loci does not allow to determine whether oat varieties belong to certain breeding centers, which is associated with the introduction of the same genotypes into the breeding programs of different regions.

Download Full-text

OL-024 Genetic diversity and nucleoside analogues resistance mutations of hepatitis B virus in Russian Federation and Viet Nam

International Journal of Infectious Diseases ◽

10.1016/s1201-9712(10)60031-8 ◽

2010 ◽

Vol 14 ◽

pp. S11

Author(s):

E.A. Elpaeva ◽

A.B. Komissarov ◽

E.A. Poretskova ◽

O.P. Dudanova ◽

D.T. Tuyên ◽

...

Keyword(s):

Genetic Diversity ◽

Hepatitis B Virus ◽

Hepatitis B ◽

Russian Federation ◽

Nucleoside Analogues ◽

Viet Nam ◽

Resistance Mutations ◽

B Virus

Download Full-text

Evaluation of genetic diversity and relationships among eight Russian and Ukrainian cattle breeds based on microsatellite markers

Regulatory Mechanisms in Biosystems ◽

10.15421/021958 ◽

2019 ◽

Vol 10 (4) ◽

pp. 388-393

Author(s):

E. A. Snegin ◽

A. S. Kramarenko ◽

E. A. Snegina ◽

S. S. Kramarenko

Keyword(s):

Genetic Diversity ◽

Genetic Polymorphism ◽

Russian Federation ◽

Microsatellite Loci ◽

Allelic Diversity ◽

Effective Population ◽

Very High Frequency ◽

Cattle Breeds ◽

The Russian Federation ◽

Very High

In recent years, special attention has been paid both to the preservation of the genetic diversity of cattle breeds in the Russian Federation and Ukraine, and to the evaluation of the breeds’ ‘purity’, especially in relation to undesirable recessive lethal mutations. The main goal of our work was to assess the genetic diversity of the main cattle breeds bred in the Russian Federation and Ukraine using microsatellite DNA loci, as well as to evaluate the phylogenetic relationships between them. An analysis of molecular genetic diversity was carried out in different cattle breeds represented both in the Russian Federation and Ukraine. A total of 752 individuals, which belonged to eight breeds of the dairy and meat productivity directions were studied. Ten microsatellite loci recommended by ISAG-FAO and arranged in one multiplex panel (ETH3, INRA023, TGLA227, BM1818, TGLA122, SPS115, TGLA53, BM2113, BM1824 and ETH10) were used as DNA markers. Some alleles were found with a very high frequency in most of the studied cattle breeds and, thus, they can be considered as species-specific for the species Bos taurus as a whole. These were alleles 262 and 266 bp (for BM1818), 180 and 188 bp (for BM1824), 125-127 and 133-135 bp (for BM2113), 217-219 bp (for ETH10), 248 and 252 bp (for SPS115). All breeds included in the analysis are characterized by a very high genetic uniqueness based on microsatellite loci; the accuracy of assigning the studied individuals to their own population varied within the range of 95–100%. In general, all breeds studied are grouped into two clusters. Cluster No. 1 contains genetic groups originating from Holstein, and cluster No. 2 contains the remaining breeds. Three breeds (Ayrshire, Simmental and Russian Red Pied) are characterized by very low estimates of the effective population size (less than 50 animals), which may lead to the loss of their genetic uniqueness in the future. In the study of eight dairy and meat cattle breeds that are bred in the Russian Federation and Ukraine, we found that the breeds differ significantly in the genetic polymorphism indicators over ten MS-DNA loci used. Moreover, the level of allelic diversity for the studied breeds was determined, primarily, by the presence and number of rare (and/or “private”) alleles. This may be due to the manifestation of different mechanisms of the formation and maintenance of genetic polymorphism in breeds, based on productivity direction.

Download Full-text