Genetic diversity and forensic parameters of 12 X-STR included in Argus X-12® marker panel in the population of the Russian Federation

2021 ◽  
pp. 1-13
Author(s):  
Andrei Semikhodskii ◽  
Yevgeniy Krassotkin ◽  
Tatiana Makarova ◽  
Vladislav Zavarin ◽  
Viktoria Ilina ◽  
...  
PIG-BREEDING ◽  
2021 ◽  
pp. 13-15
Author(s):  
BAKOEV N.F. ◽  
◽  
GETMANTSEVA L.V. ◽  

The paper presents the results of a study of the D-loop area in Landrace pigs (n=49). Amplification of the mtDNA D-loop fragment was performed according to Sanger's method. The experiments showed that modern Landrace pigs in the breeding farms of the Russian Federation are distinguished by a sufficient intra-breed diversity and have different haplotypes that belong to both the European and Asian haplogroups.


Author(s):  
D. V. Vorobiev ◽  
V. S. Solomka ◽  
K. I. Plakhova ◽  
D. G. Deryabin ◽  
A. A. Kubanov

Aim. Characteristics of contemporary strains of N. gonorrhoeae using NG-MAST genotyping (.Neisseria gonorrhoeae multi-antigen sequence typing), isolation of dominating variants and analysis of their territorial distribution. Materials and methods. 440 strains of N. gonorrhoeae isolated in 2012 - 2015 in 19 subjects of the Russian Federation were used in the study. Genotyping was carried out based on sequencing of variable regions of porB and tbpB genes. Identification of alleles and sequence-types was carried out according to http://www.ng-mast.net. Results. 172 NG-MAST genotypes were identified, 100 of which -were described for the first time. 807,1152, 1544,5714 and 5941 were predominating sequence-types, typical for several subjects of the Russian Federation and some neighboring countries, as well as sequence-types 8583 and 9476 described for the first time, that were exclusively distributed in the regions of their emergence. Sequence-types 225, 1407 and 2992, that are epidemically significant in distant countries, were represented by single isolates in this study. Conclusion. A peculiar and significant genetic diversity of N. gonorrhoeae strains, circulating in the Russian Federation, with characteristic bond of several NG-MAST genotypes to one or several regions, was shown.


2021 ◽  
Vol 36 ◽  
pp. 01015
Author(s):  
A.V. Lyubimova ◽  
D.I. Eremin ◽  
I.G. Loskutov ◽  
S.A. Levshtanov ◽  
I.B. Trifuntova

Alleles of avenin-coding loci were identified in 24 varieties of common oat origin from 6 different breeding centers of the Russian Federation. It was found that 33% of the studied varieties are homogeneous. Heterogeneous varieties contain from 2 to 10 biotypes. The analysis revealed 67 different genotypes, ten of which are found in the spectra of several varieties at once. Despite the presence of matching genotypes, all the studied varieties differ in the genetic formulas of avenin, the number and frequency of occurrence of biotypes. This makes it possible to effectively identify and distinguish oat varieties sown by Russian breeding using the method of prolamin electrophoresis. The analyzed groups of varieties are characterized by high values of genetic (0.54-0.79) and intra-population diversity, which indicates the stability of populations. The exception is varieties originating from the Irkutsk region, low values of genetic and intra-population diversity in the population of which may indicate the process of genetic erosion. Analysis of the frequency distribution of alleles of avenin-coding loci does not allow to determine whether oat varieties belong to certain breeding centers, which is associated with the introduction of the same genotypes into the breeding programs of different regions.


2019 ◽  
Vol 10 (4) ◽  
pp. 388-393
Author(s):  
E. A. Snegin ◽  
A. S. Kramarenko ◽  
E. A. Snegina ◽  
S. S. Kramarenko

In recent years, special attention has been paid both to the preservation of the genetic diversity of cattle breeds in the Russian Federation and Ukraine, and to the evaluation of the breeds’ ‘purity’, especially in relation to undesirable recessive lethal mutations. The main goal of our work was to assess the genetic diversity of the main cattle breeds bred in the Russian Federation and Ukraine using microsatellite DNA loci, as well as to evaluate the phylogenetic relationships between them. An analysis of molecular genetic diversity was carried out in different cattle breeds represented both in the Russian Federation and Ukraine. A total of 752 individuals, which belonged to eight breeds of the dairy and meat productivity directions were studied. Ten microsatellite loci recommended by ISAG-FAO and arranged in one multiplex panel (ETH3, INRA023, TGLA227, BM1818, TGLA122, SPS115, TGLA53, BM2113, BM1824 and ETH10) were used as DNA markers. Some alleles were found with a very high frequency in most of the studied cattle breeds and, thus, they can be considered as species-specific for the species Bos taurus as a whole. These were alleles 262 and 266 bp (for BM1818), 180 and 188 bp (for BM1824), 125-127 and 133-135 bp (for BM2113), 217-219 bp (for ETH10), 248 and 252 bp (for SPS115). All breeds included in the analysis are characterized by a very high genetic uniqueness based on microsatellite loci; the accuracy of assigning the studied individuals to their own population varied within the range of 95–100%. In general, all breeds studied are grouped into two clusters. Cluster No. 1 contains genetic groups originating from Holstein, and cluster No. 2 contains the remaining breeds. Three breeds (Ayrshire, Simmental and Russian Red Pied) are characterized by very low estimates of the effective population size (less than 50 animals), which may lead to the loss of their genetic uniqueness in the future. In the study of eight dairy and meat cattle breeds that are bred in the Russian Federation and Ukraine, we found that the breeds differ significantly in the genetic polymorphism indicators over ten MS-DNA loci used. Moreover, the level of allelic diversity for the studied breeds was determined, primarily, by the presence and number of rare (and/or “private”) alleles. This may be due to the manifestation of different mechanisms of the formation and maintenance of genetic polymorphism in breeds, based on productivity direction.


2020 ◽  
pp. 3-5
Author(s):  
Y. G. Zakharenko ◽  
N. A. Kononova ◽  
V. L. Fedorin ◽  
Z. V. Fomkina ◽  
K. V. Chekirda

The results of the work to create a complex of high-precision hardware for the unit of length reproduction and transferring carried out at “D. I. Mendeleyev Institute for Metrology (VNIIM)” are represented. This complex will serve as the basis for the further development of the reference base of the Russian Federation in the field of length measurements and will allow reproduction of the unit of length at two wavelengths of 633 nm and 532 nm, as well as measurements of the wavelength of laser sources in vacuum in the range from 500 to 1050 nm.


2018 ◽  
pp. 131-141 ◽  
Author(s):  
A. N. Savrukov ◽  
N. T. Savrukov ◽  
E. A. Kozlovskaya

The article analyzes the current state and level of development of publicprivate partnership (PPP) projects in the subjects of the Russian Federation. The authors conclude that a significant proportion of projects is implemented on a concession basis at the municipal level in the communal sphere. A detailed analysis of the project data showed that the structure of the projects is deformed in favor of the central regions of the Russian Federation, and a significant share in the total amount of financing falls on the transport sector. At the stage of assessing the level of development by the subjects of the Russian Federation, criteria were proposed, and index and integral indicators were used, which ensured comparability of the estimates obtained. At the end of the analysis, the regions were ranked and clustered according to the level of PPP development, which allowed to reveal the number and structure of leaders and outsiders.


2020 ◽  
pp. 105-116
Author(s):  
N. I. Shagaida

The article clarifies the concept of “agricultural holding”, using an approach to assessing the size on the basis of the total revenue of all agricultural organizations within the agricultural holding. It has been revealed that only 100 of the total number of agricultural holdings that were identified can be attributed to large business entities. They comprise about 3% of agricultural organizations in the country, while their share in the proceeds is about 37%. A large share of agricultural holdings — large business subjects under the control of Russian entities operate in one, and under the control of foreign legal entities — in three or more regions of the Russian Federation. Vertical integration within the framework of large agricultural holdings with different schemes for including the stages of processing and sale of products produced in their agricultural organizations allows them to receive advantages. Strengthening the role of large business entities in agriculture puts on the agenda the issue of differentiating approaches to taxation and state support in agriculture, depending on the size of the companies’ agricultural businesses.


2007 ◽  
pp. 63-75 ◽  
Author(s):  
A. Navoi

The article analyzes the situation with attraction of foreign direct investments (FDI) into the Russian Federation. Sharply increased inflow of international financial resources into national economy has highlighted the problem of definitions, the reasons of this phenomenon and its economic contents. The article considers methodological aspects and economic essence of modern FDI. Special accent is made on the estimation of the situation with their attraction into Russia, FDI structure and effectiveness. The conclusions about basic directions of the increase of their effectiveness in the Russian economy are formulated.


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