The Role of High Throughput Molecular Analysis of Biofluids and Tumors in Patients with Oral Cancer

Abstract Objectives The aim of the present study is to evaluate the role of immunoglobulins (IgA, IgG, and IgM) and circulating immune complexes (CIC) as tumor marker in oral cancer and precancer patients. Materials and Methods The present study was performed on 45 individuals subdivided into three groups, that is, oral precancer, oral cancer and healthy individuals, and levels of immunoglobulins, and CIC was estimated by turbidometry and ELISA method. Results In the present study, the mean serum IgA levels in oral precancer were 161.00 ( ± 118.02) mg/dL, oral cancers were 270.67 ( ± 171.44) mg/dL, and controls were 133.73 ( ± 101.31) mg/dL. Mean serum levels of IgG in oral precancer were 1,430.87 ( ± 316) mg/dL, oral cancers were 1,234.27 ( ± 365.42) mg/dL, and controls were 593.87 ( ± 323.06) mg/dL. Conclusion We found that the levels of serum IgG and IgA were elevated consistently in precancer and cancer group, and Serum IgM levels were increased only in precancer. Also, significant increase in serum CIC levels were seen in oral precancer and cancer group on comparison with control.

Download Full-text

Role of interleukin-10 promoter polymorphisms in oral cancer susceptibility: A meta-analysis

Cancer Investigation ◽

10.1080/07357907.2021.1900217 ◽

2021 ◽

pp. 1-25

Author(s):

Ru-Yue Liu ◽

Lin Li ◽

Ting Wu ◽

Zhong-Ti Zhang

Keyword(s):

Oral Cancer ◽

Cancer Susceptibility ◽

Meta Analysis ◽

Interleukin 10 ◽

Promoter Polymorphisms

Download Full-text

Fast and Efficient 5′P Degradome Library Preparation for Analysis of Co-Translational Decay in Arabidopsis

Plants ◽

10.3390/plants10030466 ◽

2021 ◽

Vol 10 (3) ◽

pp. 466

Author(s):

Marie-Christine Carpentier ◽

Cécile Bousquet-Antonelli ◽

Rémy Merret

Keyword(s):

Gene Expression ◽

Quality Control ◽

Transcriptional Regulation ◽

High Throughput ◽

Library Preparation ◽

Working Day ◽

Set Up ◽

Post Transcriptional Regulation ◽

Commercial Kit

The recent development of high-throughput technologies based on RNA sequencing has allowed a better description of the role of post-transcriptional regulation in gene expression. In particular, the development of degradome approaches based on the capture of 5′monophosphate decay intermediates allows the discovery of a new decay pathway called co-translational mRNA decay. Thanks to these approaches, ribosome dynamics could now be revealed by analysis of 5′P reads accumulation. However, library preparation could be difficult to set-up for non-specialists. Here, we present a fast and efficient 5′P degradome library preparation for Arabidopsis samples. Our protocol was designed without commercial kit and gel purification and can be easily done in one working day. We demonstrated the robustness and the reproducibility of our protocol. Finally, we present the bioinformatic reads-outs necessary to assess library quality control.

Download Full-text

ANLN promotes carcinogenesis in oral cancer by regulating the PI3K/mTOR signaling pathway

Head & Face Medicine ◽

10.1186/s13005-021-00269-z ◽

2021 ◽

Vol 17 (1) ◽

Author(s):

Bing Wang ◽

Xiao-li Zhang ◽

Chen-xi Li ◽

Ning-ning Liu ◽

Min Hu ◽

...

Keyword(s):

Oral Cancer ◽

Signaling Pathway ◽

Cell Cycle Progression ◽

Mtor Signaling ◽

Mtor Signaling Pathway ◽

Cell Behaviors ◽

Rnai Technology ◽

Tumor Tissues ◽

Pi3k Signaling Pathway

Abstract Background Oral cancer is a malignant disease that threatenshuman life and greatly reducespatientquality of life. ANLN was reported to promote the progression of cancer. This study aims to investigate the role of ANLNin oral cancer and the underlying molecular mechanism. Methods ANLN expression was downregulated by RNAi technology. The effect of ANLN on cell behaviors, including proliferation, cell cycle progression, invasion, and apoptosis, was detected. Western blotting analysis was used to explore the mechanism by whichANLN functions in oral cancer. Results Data from TCGA database showed that ANLN was expressed at significantly higher levels in tumor tissues thanin normal control tissues. Patients with higher ANLN expression exhibitedshorter survivaltimes. ANLN was alsoabundantly expressedin the cancer cell lines CAL27 and HN30. When ANLN was knocked down in CAL27 and HN30 cells, cell proliferation and colony formation weredecreased. The cell invasion ability was also inhibited. However, the cell apoptosis rate was increased. In addition, the levels of critical members of the PI3K signaling pathway, includingPI3K, mTOR, Akt, and PDK-1, were significantlyreducedafter ANLN was knocked down in CAL27 cells. Conclusions ANLN contributes to oral cancerprogressionand affects activation ofthe PI3K/mTOR signaling pathway. This study providesa new potential targetfor drug development and treatment in oral cancer.

Download Full-text