scholarly journals DNA Damage Quantitation by Alkaline Gel Electrophoresis

1999 ◽  
pp. 183-202 ◽  
Author(s):  
Betsy M. Sutherland ◽  
Paula V. Bennett ◽  
John C. Sutherland
Keyword(s):  
Dna Damage ◽  
Gel Electrophoresis

Silver Nanoparticles and Carbon Nanotubes Induced DNA Damage in Mice Evaluated by Single Cell Gel Electrophoresis

2015 ◽  
Vol 357 (1) ◽  
pp. 210-217 ◽  
Author(s):  
Kumud Kant Awasthi ◽  
Anjali Awasthi ◽  
Rajbala Verma ◽  
Inderpal Soni ◽  
Kamlendra Awasthi ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
Dna Damage ◽  
Silver Nanoparticles ◽  
Single Cell ◽  
Gel Electrophoresis ◽  
Single Cell Gel Electrophoresis

Malathion Induced DNA Damage in Freshwater Fish, Labeo rohita (Hamilton, 1822) Using Alkaline Single Cell Gel Electrophoresis

2016 ◽  
Vol 11 (2) ◽  
pp. 98-105 ◽  
Author(s):  
Sana Ullah ◽  
Maryam Begum ◽  
Kuldeep Dhama ◽  
Saeed Ahmad ◽  
Said Hassan ◽  
...  
Keyword(s):  
Dna Damage ◽  
Single Cell ◽  
Freshwater Fish ◽  
Gel Electrophoresis ◽  
Labeo Rohita ◽  
Single Cell Gel Electrophoresis

scholarly journals THE BIOCHEMICAL ALTERATION AND DNA DAMAGE IN RATS (RATTUS RATTUS) AFTER CHRONIC INTRAPERITONEALLY INJECTION TO PURIFIED MICROCYSTIN-LR FROM ANABAENA CIRCINALIS

2017 ◽  
Vol 10 (11) ◽  
pp. 277
Author(s):  
Maher M Khadairi ◽  
Moayed Jy Al-amari ◽  
Ayad Mj Al-mamoori
Keyword(s):  
Dna Damage ◽  
Gel Electrophoresis ◽  
Biochemical Markers ◽  
Chronic Exposure ◽  
High Performance ◽  
Rattus Rattus ◽  
Tail Length ◽  
Oxygen Species ◽  
Tail Moment ◽  
Single Cell Gel Electrophoresis

  Objective: This study determined the effect of purified microcystin-leucine arginine (MC-LR) on biochemical and DNA damage parameters in rats.Methods: Utilization of preparative high-performance liquid chromatography in analysis, purification and collection of MC-LR, then intraperitoneally injection of purified MC-LR to rats. At the end of exposure, animals were sacrificed, and liver cell was isolated to measure the biochemical markers such as superoxide dismutase (SOD), catalase (CAT) and glutathione (GSH) as well as measured malondialdehyde (MDA), reactive oxygen species (ROS) and cytochrome P450 (Cyt P450), and DNA damage markers such as comet length, tail length, and tail moment were measured with the single cell gel electrophoresis also called comet assay.Results: The present results showed significantly increased activities of SOD as well as concentration of MDA, ROS with increasing concentration of MC-LR but the activities of CAT and GSH, as well as Cyt P450, were significantly decreased with increasing MC-LR dose while makers of DNA damage such as comet length, tail length, and tail moment also significantly increased with increasing MC-LR dose.Conclusion: This study demonstrated that chronic exposure to MC-LR toxin can induce alteration of biochemical and DNA damage markers.

scholarly journals Inhibition of H2O2-induced DNA damage in single cell gel electrophoresis assay (comet assay) by castasterone isolated from leaves of centella asiatica

Health ◽  
2010 ◽  
Vol 02 (06) ◽  
pp. 595-602 ◽  
Author(s):  
Nishi Sondhi ◽  
Renu Bhardwaj ◽  
Satwinderjeet Kaur ◽  
Madhu Chandel ◽  
Neeraj Kumar ◽  
...  
Keyword(s):  
Dna Damage ◽  
Comet Assay ◽  
Single Cell ◽  
Gel Electrophoresis ◽  
Centella Asiatica ◽  
Single Cell Gel Electrophoresis
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