THE BIOCHEMICAL ALTERATION AND DNA DAMAGE IN RATS (RATTUS RATTUS) AFTER CHRONIC INTRAPERITONEALLY INJECTION TO PURIFIED MICROCYSTIN-LR FROM ANABAENA CIRCINALIS

Objective: This study determined the effect of purified microcystin-leucine arginine (MC-LR) on biochemical and DNA damage parameters in rats.Methods: Utilization of preparative high-performance liquid chromatography in analysis, purification and collection of MC-LR, then intraperitoneally injection of purified MC-LR to rats. At the end of exposure, animals were sacrificed, and liver cell was isolated to measure the biochemical markers such as superoxide dismutase (SOD), catalase (CAT) and glutathione (GSH) as well as measured malondialdehyde (MDA), reactive oxygen species (ROS) and cytochrome P450 (Cyt P450), and DNA damage markers such as comet length, tail length, and tail moment were measured with the single cell gel electrophoresis also called comet assay.Results: The present results showed significantly increased activities of SOD as well as concentration of MDA, ROS with increasing concentration of MC-LR but the activities of CAT and GSH, as well as Cyt P450, were significantly decreased with increasing MC-LR dose while makers of DNA damage such as comet length, tail length, and tail moment also significantly increased with increasing MC-LR dose.Conclusion: This study demonstrated that chronic exposure to MC-LR toxin can induce alteration of biochemical and DNA damage markers.

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Can single cell gel electrophoresis [comet assay] predict the outcome in neonatal sepsis?

National Journal of Clinical Anatomy ◽

10.1055/s-0040-1701730 ◽

2018 ◽

Vol 7 (03) ◽

pp. 153-156

Author(s):

Priyadharshini NA ◽

Parkash Chand ◽

Vishnu Bhatt S.

Keyword(s):

Dna Damage ◽

Comet Assay ◽

Single Cell ◽

Neonatal Sepsis ◽

Gel Electrophoresis ◽

Area Under The Curve ◽

Tail Length ◽

Single Cell Gel Electrophoresis ◽

High Area ◽

Predicting Outcome

Abstract Background & aim : Early diagnosis of neonatal sepsis is important for effective management and recovery. The available methods for diagnosis were not useful in predicting outcome. In the present study the technique of single cell gel electrophoresis [comet assay] which measures DNA damage was used to predict the outcome of neonatal sepsis. Material & methods : Comet Assay [single cell gel electrophoresis, SCGE] was used as a tool to assess the DNA damage in 3 5 term neonates with sepsis .The comet parameters were compared between those who recovered and expired due to sepsis neonatorum. Results were analysed using independent student t test with SPSS software version 19. p values <0.05 was taken as significant. Results : Comet length [174.57 ± 46.66pm] and Tail length [ 114.63 ± 52.92pm] are the prime indicator of DNA damage and were significantly [p Z < .05 ] higher among the expired cases when compared to the recovered cases [113.70 ±32.72 and 46.40±37.57 respectively]. Receiver Operating Characteristic [ROC] curve for comet length and tail length showed comet length and tail length are high. Area under the curve [AUC] 0.867 with cut off value of 140.06pm [71.4% sensitivity, 85.7% specificity] and 0.862 with cut off value of 63.12pm [85% sensitivity, 71.4% specificity] respectively. Conclusion :Weconclude that SCGE can be used for predicting mortality in neonatal sepsis

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