Use of Mathematics in Assessing Solute Exchange Across the Lung Epithelium

1990 ◽  
pp. 162-168
Author(s):  
B. T. Peterson ◽  
M. L. Collins ◽  
J. C. Connelly ◽  
J. W. McLarty ◽  
D. Holiday ◽  
...  
Keyword(s):  
Lung Epithelium ◽  
Solute Exchange

scholarly journals Mycobacterium tuberculosis Infection Up-Regulates Sialyl Lewis X Expression in the Lung Epithelium

2021 ◽  
Vol 9 (1) ◽  
pp. 99
Author(s):  
Rita Matos ◽  
Kaori L. Fonseca ◽  
Stefan Mereiter ◽  
Ana Raquel Maceiras ◽  
Joana Gomes ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Biosynthetic Pathway ◽  
Molecular Mechanisms ◽  
Tuberculosis Infection ◽  
Host Susceptibility ◽  
Sialyl Lewis X ◽  
Lung Pathology ◽  
Lung Epithelium ◽  
Lewis X ◽  
Sialyl Lewis

Glycans display increasingly recognized roles in pathological contexts, however, their impact in the host-pathogen interplay in many infectious diseases remains largely unknown. This is the case for tuberculosis (TB), one of the ten most fatal diseases worldwide, caused by infection of the bacteria Mycobacterium tuberculosis. We have recently reported that perturbing the core-2 O-glycans biosynthetic pathway increases the host susceptibility to M. tuberculosis infection, by disrupting the neutrophil homeostasis and enhancing lung pathology. In the present study, we show an increased expression of the sialylated glycan structure Sialyl-Lewis X (SLeX) in the lung epithelium upon M. tuberculosis infection. This increase in SLeX glycan epitope is accompanied by an altered lung tissue transcriptomic signature, with up-regulation of genes codifying enzymes that are involved in the SLeX core-2 O-glycans biosynthetic pathway. This study provides novel insights into previously unappreciated molecular mechanisms involving glycosylation, which modulate the host response to M. tuberculosis infection, possibly contributing to shape TB disease outcome.

Solute Exchange by Convection Within Estuarine Sediments

1996 ◽  
Vol 42 (2) ◽  
pp. 171-183 ◽  
Author(s):  
I.T. Webster ◽  
S.J. Norquay ◽  
F.C. Ross ◽  
R.A. Wooding
Keyword(s):  
Estuarine Sediments ◽  
Solute Exchange

β2-Agonist regulation of cell volume in fetal distal lung epithelium by CAMP-independent Ca2+ release from intracellular stores

1997 ◽  
Vol 75 (8) ◽  
pp. 1030-1033 ◽  
Author(s):  
N Niisato ◽  
T Nakahari ◽  
A K Tanswell ◽  
Y Marunaka
Keyword(s):  
Cell Volume ◽  
Lung Epithelium ◽  
Distal Lung ◽  
Β2 Agonist

The response of lung epithelium to well characterised fine particles

Life Sciences ◽  
1998 ◽  
Vol 62 (19) ◽  
pp. 1789-1799 ◽  
Author(s):  
Samantha A. Murphy ◽  
Kelly A. Bérubé ◽  
Frederick D. Pooley ◽  
Roy J. Richards
Keyword(s):  
Fine Particles ◽  
Lung Epithelium

Effect of Covalent Serpin–Heparinoid Complexes on Plasma Thrombin Generation on Fetal Distal Lung Epithelium

2003 ◽  
Vol 28 (2) ◽  
pp. 150-158 ◽  
Author(s):  
Leslie R. Berry ◽  
Petr Klement ◽  
Maureen Andrew ◽  
Anthony K. C. Chan
Keyword(s):  
Thrombin Generation ◽  
Lung Epithelium ◽  
Distal Lung

Basolateral Cl− uptake mechanisms in Xenopus laevis lung epithelium

2010 ◽  
Vol 299 (1) ◽  
pp. R92-R100 ◽  
Author(s):  
Jens Berger ◽  
Martin Hardt ◽  
Wolfgang G. Clauss ◽  
Martin Fronius
Keyword(s):  
Xenopus Laevis ◽  
Ion Transport ◽  
Anion Exchanger ◽  
Short Circuit ◽  
Ussing Chamber ◽  
Short Circuit Current ◽  
Transport Processes ◽  
Lung Epithelium ◽  
Active Ion Transport ◽  
Uptake Mechanisms

A thin liquid layer covers the lungs of air-breathing vertebrates. Active ion transport processes via the pulmonary epithelial cells regulate the maintenance of this layer. This study focuses on basolateral Cl− uptake mechanisms in native lungs of Xenopus laevis and the involvement of the Na+/K+/2 Cl− cotransporter (NKCC) and HCO3−/Cl− anion exchanger (AE), in particular. Western blot analysis and immunofluorescence staining revealed the expression of the NKCC protein in the Xenopus lung. Ussing chamber experiments demonstrated that the NKCC inhibitors (bumetanide and furosemide) were ineffective at blocking the cotransporter under basal conditions, as well as under pharmacologically stimulated Cl−-secreting conditions (forskolin and chlorzoxazone application). However, functional evidence for the NKCC was detected by generating a transepithelial Cl− gradient. Further, we were interested in the involvement of the HCO3−/Cl− anion exchanger to transepithelial ion transport processes. Basolateral application of DIDS, an inhibitor of the AE, resulted in a significantly decreased the short-circuit current (ISC). The effect of DIDS was diminished by acetazolamide and reduced by increased external HCO3− concentrations. Cl− secretion induced by forskolin was decreased by DIDS, but this effect was abolished in the presence of HCO3−. These experiments indicate that the AE at least partially contributes to Cl− secretion. Taken together, our data show that in Xenopus lung epithelia, the AE, rather than the NKCC, is involved in basolateral Cl− uptake, which contrasts with the common model for Cl− secretion in pulmonary epithelia.

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