Inhibitory Effect of Indomethacin on Nuclear Binding of Progesterone in the Uterus and on Differentiation of Decidual Cells in Culture

1981 ◽  
pp. 431-433
Author(s):  
Sara Peleg ◽  
Hans R. Lindner
Keyword(s):  
Inhibitory Effect ◽  
Nuclear Binding ◽  
Cells In Culture ◽  
Decidual Cells

Effects of prostaglandins E1, E2, and F2alpha on the growth of leukaemia cells in culture

1976 ◽  
Vol 20 (1) ◽  
pp. 199-206
Author(s):  
T.J. Yang ◽  
J.B. Dale ◽  
R. Machanoff
Keyword(s):  
Tritiated Thymidine ◽  
Inhibitory Effect ◽  
Soft Agar ◽  
Significant Inhibition ◽  
Leucine Incorporation ◽  
Inhibitory Effects ◽  
Uridine Incorporation ◽  
Cells In Culture ◽  
Mouse Leukaemia

Prostaglandins E1, E2, and F2alpha (PGE1, PGE2, and PGF2alpha) were shown to inhibit the growth of mouse leukaemia lymphoblasts L5178Y in culture. The effects of PGE1 and PGE2 were greater than that of PGF2alpha. PGE1 and PGE2, at the concentration of 100 mug per ml showed significant inhibitory effects on the rates of incorporation of tritiated thymidine, uridine and leucine. At concentrations of 50 and 25 mug per ml, there was significant inhibition of thymidine and uridine incorporation, but not of leucine, PGF2alpha showed significant inhibition of thymidine and uridine incorporation but not leucine incorporation, in all 3 concentrations studied (100, 50, and 25 mug/ml). The ability of the cells to form colonies in soft agar was significantly inhibited by PGE1 and PGE2 at concentrations as low as 1–8 mug/ml. For F2alpha, however, a concentration as high as 56mug/ml was required to show inhibitory effect, but at 1–8 mug/ml it was found to be stimulatory.

scholarly journals Transforming Growth Factor-βs Inhibit Somatostatin Messenger Ribonucleic Acid Levels and Somatostatin Secretion in Hypothalamic Cells in Culture*

Endocrinology ◽  
1997 ◽  
Vol 138 (10) ◽  
pp. 4401-4409 ◽  
Author(s):  
M. Quintela ◽  
R. M. SeñarÍs ◽  
C. Diéguez
Keyword(s):  
Growth Factor ◽  
Messenger Rna ◽  
Transforming Growth Factor ◽  
Inhibitory Effect ◽  
Mrna Levels ◽  
Messenger Ribonucleic Acid ◽  
Cells In Culture ◽  
Somatostatin Secretion ◽  
Hypothalamic Cells ◽  
The Stability

Abstract Treatment of hypothalamic cells in monolayer culture with transforming growth factor-β1 (TGFβ1) significantly reduced both basal and cAMP-induced somatostatin messenger RNA (mRNA) levels and somatostatin secretion. This inhibitory effect was dose- and time-dependent and not mediated by glial cells, as it was also observed in glial-free hypothalamic cell cultures treated with cytosine arabinonucleoside. TGFβ2 and -β3 mimicked the actions of TGFβ1, which indicated that the three isoforms of the TGFβ family expressed in the central nervous system displayed similar effects on the somatostatinergic neurons. The blockade of synthesis of proteins with either cycloheximide or puromycin for 24 h prevented the inhibitory effect of TGFβ1 on somatostatin mRNA. This implied that the reduction of this mRNA by TGFβ1 required de novo protein synthesis. We next studied whether TGFβ1 acted at the transcriptional or posttranscriptional level by altering the stability of somatostatin mRNA. Examination of the rate of disappearance of somatostatin mRNA by Northern blot, after inhibition of mRNA transcription with either actinomycin D (AcD) or 5,6-dichloro-1β-ribofuranosyl benzimidazole revealed that TGFβ1 did reduce the stability of somatostatin mRNA. This effect was observed when we pretreated the cultures with TGFβ1 4 h before the addition of AcD, but not when we administered TGFβ1 simultaneously with AcD or 5,6-dichloro-1β-ribofuranosyl benzimidazole. Altogether these results demonstrated that the treatment of hypothalamic cells in culture with TGFβ1, TGFβ2, or TGFβ3 resulted in a decrease in somatostatin mRNA levels and somatostatin secretion. TGFβ1 reduced the steady state levels of somatostatin mRNA by inducing the synthesis of a protein (s), that appears to accelerate the degradation of the mRNA of somatostatin. Whether TGFβ1 has additional effects on the transcription of the somatostatin gene will require further study.

scholarly journals Inhibitory Effect of Heparin on Collagen Fiber Formation in Hepatic Cells in Culture.

1997 ◽  
Vol 22 (5) ◽  
pp. 533-538 ◽  
Author(s):  
Kazumi Akimoto ◽  
Masashi Ikeda ◽  
Kenji Sorimachi
Keyword(s):  
Collagen Fiber ◽  
Inhibitory Effect ◽  
Fiber Formation ◽  
Cells In Culture ◽  
Hepatic Cells

Effect of GnRH-associated peptide on prolactin secretion from human lactotrope adenoma cells in culture

1987 ◽  
Vol 116 (1) ◽  
pp. 81-84 ◽  
Author(s):  
Miyuki Ishibashi ◽  
Tohru Yamaji ◽  
Fumimaro Takaku ◽  
Akira Teramoto ◽  
Takanori Fukushima ◽  
...  
Keyword(s):  
Pituitary Adenomas ◽  
Inhibitory Effect ◽  
Prolactin Secretion ◽  
Significant Inhibition ◽  
The Other ◽  
Human Pituitary ◽  
Gh Secretion ◽  
Cells In Culture ◽  
Other Hand ◽  
Transsphenoidal Adenomectomy

Abstract. The effect of GnRH-associated peptide on PRL secretion by human pituitary lactotropes in culture was studied. Pituitary adenomas obtained at selective transsphenoidal adenomectomy from a patient with prolactinoma, and two patients with mixed GH- and PRL-secreting pituitary adenomas were cultured in monolayer. When cells were incubated with dopamine (10 nmol/l), a significant inhibition in PRL secretion was observed in all the experiments, which was blocked by co-incubation with haloperidol. In mixed GH- and PRL-secreting adenoma cells, dopamine likewise decreased GH secretion. Incubation of cells with synthetic GnRH-associated peptide at concentrations up to 100 nmol/l, on the other hand, failed to affect both PRL and GH secretion. These results suggest that synthetic GnRH-associated peptide has no inhibitory effect on PRL secretion in human pituitary lactotropes.

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