scholarly journals Fermentation Study on Macroalgae Eucheuma cottonii for Bioethanol Production via Varying Acid Hydrolysis

2012 ◽  
pp. 219-240 ◽  
Author(s):  
Rachel Fran Mansa ◽  
Wei-Fang Chen ◽  
Siau-Jen Yeo ◽  
Yan-Yan Farm ◽  
Hafeza Abu Bakar ◽  
...  
2017 ◽  
Vol 39 (4) ◽  
pp. 423 ◽  
Author(s):  
George Meredite Cunha de Castro ◽  
Norma Maria Barros Benevides ◽  
Maulori Curié Cabral ◽  
Rafael De Souza Miranda ◽  
Enéas Gomes Filho ◽  
...  

 The seaweeds are bio-resource rich in sulfated and neutral polysaccharides. The tropical seaweed species used in this study (Solieria filiformis), after dried, shows 65.8% (w/w) carbohydrate, 9.6% (w/w) protein, 1.7% (w/w) lipid, 7.0% (w/w) moisture and 15.9% (w/w) ash. The dried seaweed was easily hydrolyzed under mild conditions (0.5 M sulfuric acid, 20 min.), generating fermentable monosaccharides with a maximum hydrolysis efficiency of 63.21%. Galactose and glucose present in the hydrolyzed were simultaneously fermented by Saccharomyces cerevisiae when the yeast was acclimated to galactose and cultivated in broth containing only galactose. The kinetic parameters of the fermentation of the seaweed hydrolyzed were Y(P⁄S) = 0.48 ± 0.02 g.g−1, PP = 0.27 ± 0.04 g.L−1.h−1, h = 94.1%, representing a 41% increase in bioethanol productivity. Therefore, S. filiformis was a promising renewable resource of polysaccharides easily hydrolyzed, generating a broth rich in fermentable monosaccharides for ethanol production. 


ALCHEMY ◽  
2018 ◽  
Vol 6 (1) ◽  
pp. 24
Author(s):  
Dewi Yuliani ◽  
Khoirul Achmad Julianto ◽  
Akyunul Jannah

<p class="BodyAbstract">Rice bran is one among many agricultural by-products containing ~50-60 wt.% of carbohydrate. The carbohydrate is a prominent sugar source for bioethanol production. The objective of this research was to study bioethanol production from rice bran by acid and enzymatic treatment. The variations of acid used were dilute hydrochloric acid and sulphuric acid, while variations of enzyme used were amylolytic and cellulolytic enzyme. Ethanol production of acid-hydrolyzed rice bran was 24.95±1.61% (v/v) by hydrochloric acid and 29.57±2.04% (v/v) by sulphuric acid. Ethanol produced by enzymatic hydrolysis was quite low i.e. 6.7±0.04%, and 8.86±0.29% (v/v) for amylolytic and cellulolytic hydrolysate, respectively.</p><p class="BodyAbstract"> </p><p>Keywords: Bioethanol, rice bran, acid hydrolysis, enzymatic hydrolysis</p>


2011 ◽  
Vol 102 (14) ◽  
pp. 7119-7123 ◽  
Author(s):  
Rajendran Velmurugan ◽  
Karuppan Muthukumar

2020 ◽  
Vol 2020 ◽  
pp. 1-11
Author(s):  
Sefrinus Maria Dolfi Kolo ◽  
Deana Wahyuningrum ◽  
Rukman Hertadi

The process of acid hydrolysis using conventional methods at high concentrations results in products having lower yields, and it needs a longer time of process; therefore, it becomes less effective. In this study, we analyzed the effects of microwave-assisted pretreatment and cofermentation on bioethanol production from elephant grass (Pennisetum purpureum). We used a combination of delignification techniques and acid hydrolysis by employing a microwave-assisted pretreatment method on elephant grass (Pennisetum purpureum) as a lignocellulosic material. This was followed by cofermentation with Saccharomyces cerevisiae ITB-R89 and Pichia stipitis ITB-R58 to produce bioethanol. The optimal sugar mixtures (fructose and xylose) of the hydrolysis product were subsequently converted into bioethanol by cofermentation with S. cerevisiae ITB-R89 and P. stipitis ITB-R58, carried out with varying concentrations of inoculum for 5 days (48 h) at 30°C and pH 4.5. The high-power liquid chromatographic analysis revealed that the optimal inoculum concentration capable of converting 76.15% of the sugar mixture substrate (glucose and xylose) to 10.79 g/L (34.74% yield) of bioethanol was 10% (v/v). The optimal rate of ethanol production was 0.45 g/L/d, corresponding to a fermentation efficiency of 69.48%.


2009 ◽  
Vol 43 (2) ◽  
pp. 208-211 ◽  
Author(s):  
Marija B. Tasić ◽  
Budimir V. Konstantinović ◽  
Miodrag L. Lazić ◽  
Vlada B. Veljković

2013 ◽  
Vol 7 (3) ◽  
pp. 376-389 ◽  
Author(s):  
Can Ucuncu ◽  
Canan Tari ◽  
Hande Demir ◽  
Ali Oguz Buyukkileci ◽  
Banu Ozen

2017 ◽  
Vol 28 (3) ◽  
pp. 1 ◽  
Author(s):  
Charlie Marembu Dodo ◽  
Samphson Mamphweli ◽  
Omobola Okoh

Bioethanol production is one of the most promising possible substitutes for fossil-based fuels, but there is a need to make available cost-effective methods of production if it is to be successful. Various methods for the production of bioethanol using different feedstocks have been explored. Bioethanol synthesis from sugarcane, their tops and leaves have generally been regarded as waste and discarded. This investigation examined the use of lignocellulosic sugarcane leaves and tops as biomass and evaluated their hydrolysate content. The leaves and tops were hydrolysed using concentrated and dilute sulphuric acid and compared with a combination of oxidative alkali-peroxide pre-treatment with enzyme hydrolysis using the enzyme cellulysin® cellulase. Subsequent fermentation of the hydrolysates into bioethanol was done using the yeast saccharomyces cerevisae. The problem of acid hydrolysis to produce inhibitors was eliminated by overliming using calcium hydroxide and this treatment was subsequently compared with sodium hydroxide neutralisation. It was found that oxidative alkali pre-treatment with enzyme hydrolysis gave the highest yield of fermentable sugars of 38% (g/g) for 7% (v/v) peroxide pretreated biomass than 36% (g/g) for 5% (v/v) with the least inhibitors. Concentrated and dilute acid hydrolysis each gave yields of 25% (g/g) and 22% (g/g) respectively, although the acid required a neutralisation step, resulting in dilution. Alkaline neutralisation of acid hydrolysates using sodium hydroxide resulted in less dilution and loss of fermentable sugars, compared with overliming. Higher yields of bioethanol of 13.7 g/l were obtained from enzyme hydrolysates than the 6.9 g/l ethanol from dilute acid hydrolysates. There was more bioethanol yield of 13.7 g/l after 72 hours of fermentation with the yeast than the 7.0 g/l bioethanol after 24 hours.This research showed that it is possible to use sugarcane waste material to supplement biofuel requirements and that combining the chemical and biological methods of pretreatments can give higher yields at a faster rate.


2016 ◽  
Vol 3 (3) ◽  
pp. 107
Author(s):  
Wagiman . ◽  
Makhmudun Ainuri ◽  
Rinda Gusvita ◽  
Jumeri .

<p>The aim of this research was study of E. cottonii to produce bioethanol fermentation substrate with a high reduction sugar content and low Hidroxymethilfurfural (HMF). Fermentation done by instant yeast and Saccharomyces cerevisiae culture of FNCC 3012.The best treatment was obtained in the combination of 2% of H2SO4 by time reaction of 120 minutes in 80°C produced 15.61 g/l reducing sugar and 5.03 g/l HMF. In fermented process, the hydrolysate with instant yeast starter delivered much more efficiency in 3.63 ml CO2 volume, 87.53% in fermentation efficiency, and 1.96 g/l reducing sugar on fifth day of fermentation. <br /><strong>Keywords</strong>: bioethanol, Eucheuma cottonii, fermentation, hydrolysis, process design</p>


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