The Application of an Alpha Autoradiographic Technique for Determination of Boron-10 Concentrations in a Study of Intratumoral Injection of BSH and CBU-2’

1993 ◽  
pp. 449-451
Author(s):  
Yirun Jiang ◽  
Sherry Ng ◽  
Thomas E. Blue ◽  
Joan Rotaru ◽  
Rolf F. Barth
Keyword(s):  
Intratumoral Injection ◽  
Autoradiographic Technique

scholarly journals Activation Autoradiography: Imaging and Quantitative Determination of Endogenous and Exogenous Oxygen in the Rat Brain

1987 ◽  
Vol 7 (3) ◽  
pp. 272-279 ◽  
Author(s):  
Koichiro Kawashima ◽  
Ren Iwata ◽  
Kyuya Kogure ◽  
Hitoshi Ohtomo ◽  
Hikonojo Orihara ◽  
...  
Keyword(s):  
White Matter ◽  
Quantitative Determination ◽  
Rat Brain ◽  
Present Method ◽  
Hippocampal Formation ◽  
The Individual ◽  
Autoradiographic Technique ◽  
Activation Autoradiography ◽  
Local Oxygen

Endogenous and exogenous oxygen in the rat brain were quantitatively determined using an autoradiographic technique. The oxygen images of frozen and dried rat brain sections were obtained as 18F images by using the 16O (3He,p)18F reaction for endogenous 16O images and the 18O(p,n)18F reaction for endogenous and exogenous 18O images. These autoradiograms demonstrated the different distribution of oxygen between gray and white matter. These images also allowed differentiation of the individual structures of hippocampal formation, owing to the differing water content of the various structures. Local oxygen contents were quantitatively determined from autoradiograms of brain sections and standard sections with known oxygen contents. The estimated values were 75.6 ± 4.6 wt% in gray matter and 72.2 ± 4.0 wt% in white matter. The systematic error in the present method was estimated to be 4.9%.

scholarly journals Detection of surface-bound ligands by freeze-fracture autoradiography.

1985 ◽  
Vol 101 (3) ◽  
pp. 887-890 ◽  
Author(s):  
J L Carpentier ◽  
D Brown ◽  
B Iacopetta ◽  
L Orci
Keyword(s):  
Cholera Toxin ◽  
Accurate Determination ◽  
Freeze Fracture ◽  
Standard Technique ◽  
Coated Pits ◽  
Experimental Conditions ◽  
Surface Distribution ◽  
Surface Localization ◽  
Autoradiographic Technique

This article describes a new freeze-fracture autoradiographic technique for the detection of radioactive ligands associated with the surface of cells in monolayer or suspension culture. Since freeze-fracture replicas are produced in the conventional way, all membrane features normally seen in freeze-fracture are retained, and autoradiographic grains produced by the labeled ligands are seen superimposed on unaltered exoplasmic membrane fracture faces. To assess the feasibility and resolution of this technique, we compared the surface distribution of alpha 2-macroglobulin and cholera toxin, labeled either with 125I or with colloidal gold, on 3T3-L1 fibroblasts. Both by autoradiography and cytochemical gold labeling, alpha 2-macroglobulin was associated specifically with coated pits, whereas cholera toxin was preferentially found over smaller, apparently non-coated membrane invaginations. Together with data on the surface localization of 125I-transferrin on HL-60 myelomonocytic cells, these results demonstrate the application of this technique for the accurate determination of ligand distribution over large areas of plasma membrane. The simplicity and reproducibility of the method should now allow freeze-fracture autoradiography to become a standard technique for investigating the distribution of both endogenous and exogenous cell surface-associated molecules, as well as the redistribution of such molecules under different experimental conditions.

Effects of intralesional 32P in refractory patients with head and neck tumors

2007 ◽  
Vol 25 (18_suppl) ◽  
pp. 16529-16529
Author(s):  
W. C. Dempke ◽  
B. Gunawardena ◽  
B. Hesselt ◽  
R. Linke ◽  
K. A. Alimi
Keyword(s):  
Side Effects ◽  
Head And Neck ◽  
Treatment Strategies ◽  
Intratumoral Injection ◽  
Head And Neck Tumors ◽  
Cytostatic Drug ◽  
Sonographic Guidance ◽  
Neck Tumors ◽  
Novel Treatment Strategies

16529 Background: The clinical outcome of refractory head and neck cancer patients remains poor despite novel treatment strategies. In this pilot study we investigated the efficacy of intratumoral injection of 32-P chromic phosphate in 14 patients with refractory head and neck cancers in terms of response rates and overall survival. Methods: Fourteen patients (median age: 59 years) with either cytostatic drug-resistant tumors or tumors known to be primarily chemotherapy-resistant were entered into the study. After sonographic determination of the tumor volume, 32-P chromic phosphate (74–555 MBq) was injected into the central part of the tumor under sonographic guidance. Follow-up investigations included serial scintigraphy, sonographic examinations, and hematologic studies. Results: Injection of 32-P chromic phosphate into refractory head and neck tumors resulted in remarkable regression. The median survival of all patients was 7.8 months (range: 4–16). The response rate (partial response) was 57% (8 patients). However, 6 patients (43%) did not respond to the treatment. In 3 patients thrombocytopenia (grade I/II) was observed, but no other side effects were apparent. Significant pathologic and anatomic changes within the tumor tissue were demonstrated. In all cases examined, formation of a cyst within the area of central activity, surrounded by a centrifugal necrotic ring and a marginal fibrotic structure, was found. Conclusions: Lack of persistent systemic or local side effects, as well as noteworthy efficacy, are properties of this novel regional treatment modality with 32-P chromic phosphate. This modality deserves consideration for further clinical trials. No significant financial relationships to disclose.

The intrarenal distribution of urea-14C as shown by autoradiography

1968 ◽  
Vol 46 (2) ◽  
pp. 281-283 ◽  
Author(s):  
Robert D. Baynton ◽  
Paul F. Mercer
Keyword(s):  
Osmotic Pressure ◽  
Similar Pattern ◽  
Outer Zone ◽  
The Other ◽  
Constant Infusion ◽  
Outer Medulla ◽  
Mammalian Kidney ◽  
Autoradiographic Technique ◽  
Intrarenal Distribution

By use of an autoradiographic technique sodium and chloride were reported to be distributed in two separate regions in the mammalian kidney, the outer medulla and the papillary tip. Since urea is thought to be the other major component contributing to the overall osmotic pressure of the medulla, a similar pattern to that of sodium and chloride might be expected. Autoradiograms with urea-14C were made from kidneys of rats given a constant infusion of radioactivity and inulin (for the determination of GFR). The urea-14C autoradiograms showed an elevated region of radioactivity in the outer medulla adjacent to a region of lowered deposition in the outer zone of the inner medulla. This latter region was then followed, in the inner medulla, by an increasing deposition of urea-14C towards the papillary tip. This pattern is identical with that previously reported for sodium and chloride, suggesting a similar pattern for the osmotic pressure in the kidney.

Galactic orbits of stars

1966 ◽  
Vol 25 ◽  
pp. 93-97
Author(s):  
Richard Woolley
Keyword(s):  
Globular Cluster ◽  
Potential Field ◽  
High Velocity ◽  
Velocity Vector ◽  
Variable Stars ◽  
The Sun ◽  
Proper Motions ◽  
Rr Lyrae ◽  
The Galaxy

It is now possible to determine proper motions of high-velocity objects in such a way as to obtain with some accuracy the velocity vector relevant to the Sun. If a potential field of the Galaxy is assumed, one can compute an actual orbit. A determination of the velocity of the globular clusterωCentauri has recently been completed at Greenwich, and it is found that the orbit is strongly retrograde in the Galaxy. Similar calculations may be made, though with less certainty, in the case of RR Lyrae variable stars.

Distance to SN 1987A and the LMC

1999 ◽  
Vol 190 ◽  
pp. 549-554
Author(s):  
Nino Panagia
Keyword(s):  
Angular Size ◽  
Large Magellanic Cloud ◽  
Magellanic Cloud ◽  
Light Curves ◽  
Distance Modulus ◽  
Absolute Size ◽  
Sn 1987A

Using the new reductions of the IUE light curves by Sonneborn et al. (1997) and an extensive set of HST images of SN 1987A we have repeated and improved Panagia et al. (1991) analysis to obtain a better determination of the distance to the supernova. In this way we have derived an absolute size of the ringRabs= (6.23 ± 0.08) x 1017cm and an angular sizeR″ = 808 ± 17 mas, which give a distance to the supernovad(SN1987A) = 51.4 ± 1.2 kpc and a distance modulusm–M(SN1987A) = 18.55 ± 0.05. Allowing for a displacement of SN 1987A position relative to the LMC center, the distance to the barycenter of the Large Magellanic Cloud is also estimated to bed(LMC) = 52.0±1.3 kpc, which corresponds to a distance modulus ofm–M(LMC) = 18.58±0.05.

International determination of the total motion of the pole

1961 ◽  
Vol 13 ◽  
pp. 29-41
Author(s):  
Wm. Markowitz
Keyword(s):  
Secular Motion ◽  
The Future

A symposium on the future of the International Latitude Service (I. L. S.) is to be held in Helsinki in July 1960. My report for the symposium consists of two parts. Part I, denoded (Mk I) was published [1] earlier in 1960 under the title “Latitude and Longitude, and the Secular Motion of the Pole”. Part II is the present paper, denoded (Mk II).

Time and Latitude in South Africa

1972 ◽  
Vol 1 ◽  
pp. 27-38
Author(s):  
J. Hers
Keyword(s):  
South Africa ◽  
Cape Town ◽  
Astronomical Observations ◽  
Royal Observatory ◽  
The Republic ◽  
Astronomical Determination ◽  
Limited Accuracy ◽  
Better Than

In South Africa the modern outlook towards time may be said to have started in 1948. Both the two major observatories, The Royal Observatory in Cape Town and the Union Observatory (now known as the Republic Observatory) in Johannesburg had, of course, been involved in the astronomical determination of time almost from their inception, and the Johannesburg Observatory has been responsible for the official time of South Africa since 1908. However the pendulum clocks then in use could not be relied on to provide an accuracy better than about 1/10 second, which was of the same order as that of the astronomical observations. It is doubtful if much use was made of even this limited accuracy outside the two observatories, and although there may – occasionally have been a demand for more accurate time, it was certainly not voiced.

Large-scale Motion of Solar Filaments

2000 ◽  
Vol 179 ◽  
pp. 205-208
Author(s):  
Pavel Ambrož ◽  
Alfred Schroll
Keyword(s):  
Magnetic Flux ◽  
Proper Motion ◽  
Time Scale ◽  
Large Scale ◽  
Accuracy Of Measurements ◽  
Solar Filaments ◽  
General Movement ◽  
Scale Motion ◽  
Velocity Scatter

AbstractPrecise measurements of heliographic position of solar filaments were used for determination of the proper motion of solar filaments on the time-scale of days. The filaments have a tendency to make a shaking or waving of the external structure and to make a general movement of whole filament body, coinciding with the transport of the magnetic flux in the photosphere. The velocity scatter of individual measured points is about one order higher than the accuracy of measurements.

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