Interactions between Agrobacterium Tumefaciens and Plant Cells

1998 ◽  
pp. 207-229 ◽  
Author(s):  
Paul Bundock ◽  
Paul Hooykaas
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Plant Cells

Identification of the signal molecules produced by wounded plant cells that activate T-DNA transfer in Agrobacterium tumefaciens

Nature ◽  
1985 ◽  
Vol 318 (6047) ◽  
pp. 624-629 ◽  
Author(s):  
Scott E. Stachel ◽  
Eric Messens ◽  
Marc Van Montagu ◽  
Patricia Zambryski
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Plant Cells ◽  
Dna Transfer ◽  
Signal Molecules

scholarly journals Introduksi Gen DefH9-iaaM dan DefH9-RI-iaaM ke dalam Genom Tanaman Tomat Menggunakan Vektor Agrobacterium tumefaciens

2016 ◽  
Vol 6 (1) ◽  
pp. 18
Author(s):  
Ragapadmi S Purnamaningsih
Keyword(s):  
Acetic Acid ◽  
Agrobacterium Tumefaciens ◽  
Genetic Improvement ◽  
Plant Cells ◽  
Fruit Production ◽  
Total Efficiency ◽  
Parthenocarpic Fruit ◽  
Tomato Genome ◽  
Tomato Cultivars ◽  
Indole 3 Acetic Acid

<p>Introduction of DefH9-iaaM and DefH9-RI-iaaM Gene<br />Into Tomato Genome Using Agrobacterium tumefaciens.<br />Ragapadmi Purnamaningsih. Plant genetic improvement<br />can be conducted through genetic engineering.<br />Parthenocarpic fruit production could increase fruit<br />production and its qulities. IAA genes were introduced into<br />three tomato cultivars Ratna, Opal and LV 6117 using two<br />constract genes DefH9-iaaM and DefH9-RI-iaaM. The iaaM<br />gene is able to increase auxin biosynthesis in transgenic<br />plant cells and organs because indol-eacetamide,<br />synthesized by the product of the iaaM gene, is converted<br />either chemically or enzimatically to indole-3-acetic acid<br />(IAA), while the promotor DefH9 enable IAA gene expressed<br />specifically in the ovules. The objectives of this experiment<br />was to identify gene introduction into plant genom of three<br />tomato cultivars. The factors tested were two constract of<br />IAA genes (DefH9-iaaM or DefH9-RI-iaaM), tomato cultivars<br />(Ratna, Opal, and LV 6117) and time of explant inoculation<br />(5, 15, 30 minute). The result showed that the best time<br />inoculation was 5 minute. Otherwise three tomato cultivars<br />response better to DefH9-RI-iaaM than DefH9-iaaM. The total<br />efficiency of regeneration and total efficiency of<br />transformation of both genes were 25.38% and 20.32%. PCR<br />analysis showed that 10 plant have positive PCR, were 1<br />plant carried (Opal) DefH9-iaaM gene and 9 plant (Ratna,<br />Opal, LV 6117) carried DefH9-RI-iaaM gene.</p>

Gene-Expression Following T-DNA Transfer Into Plant Cells Is Aphidicolin-Sensitive

1994 ◽  
Vol 21 (2) ◽  
pp. 125 ◽  
Author(s):  
AM Chaudhury ◽  
ES Dennis ◽  
RIS Brettell
Keyword(s):  
Gene Expression ◽  
Dna Replication ◽  
Agrobacterium Tumefaciens ◽  
Plant Cells ◽  
Nicotiana Plumbaginifolia ◽  
Dna Transfer ◽  
Host Cells ◽  
35S Promoter ◽  
Glucuronidase Activity ◽  
Pass Through

A transient assay for gene-expression was used to study the early events of T-DNA transfer. Particularly, it was asked if gene expression following T-DNA transfer required DNA replication in the host cell. A β-glucuronidase gene, linked to a CaMV 35S promoter (35S-GUS, engineered so that it was inactive in Agrobacterium tumefaciens) was introduced into Nicotiana plumbaginifolia protoplasts via a disarmed supervirulent strain of Agrobacterium tumefaciens. High β-glucuronidase activity appeared after 3 days of co-cultivation. The activity required the presence of the vir functions of agrobacteria. The activity was drastically reduced if the plant cells were treated with aphidicolin, an inhibitor of DNA replication in eukaryotic cells. While double-stranded (ds) 35S-GUS DNA, introduced by electroporation, showed undiminished expression in the presence of aphidicolin, gene expression from single-stranded (ss) 35S-GUS DNA was inhibited by aphidicolin. These results suggest that DNA replication in host cells is not required for gene expression if ds-DNA is introduced by electroporation, but is required if ss-DNA is introduced by electroporation, or if DNA is transferred via A. tumefaciens. The findings are consistent with a model of T-DNA transfer in which ss-DNA molecules, once introduced into plant cells, must pass through an aphidicolin sensitive step before they can be transcribed. The simplest interpretation is that the ss-DNA is replicated by the host cell's aphidicolin-sensitive DNA polymerase before being integrated into the host genome.

scholarly journals Membrane and Core Periplasmic Agrobacterium tumefaciens Virulence Type IV Secretion System Components Localize to Multiple Sites around the Bacterial Perimeter during Lateral Attachment to Plant Cells

mBio ◽  
2011 ◽  
Vol 2 (6) ◽  
Author(s):  
Julieta Aguilar ◽  
Todd A. Cameron ◽  
John Zupan ◽  
Patricia Zambryski
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Plant Cells ◽  
Secretion System ◽  
Type Iv Secretion ◽  
Host Cells ◽  
Type Iv Secretion System ◽  
Secretion Systems ◽  
Content Type ◽  
Protein Substrates ◽  
Type Iv

ABSTRACTType IV secretion systems (T4SS) transfer DNA and/or proteins into recipient cells. Here we performed immunofluorescence deconvolution microscopy to localize the assembled T4SS by detection of its native components VirB1, VirB2, VirB4, VirB5, VirB7, VirB8, VirB9, VirB10, and VirB11 in the C58 nopaline strain ofAgrobacterium tumefaciens, following induction of virulence (vir) gene expression. These different proteins represent T4SS components spanning the inner membrane, periplasm, or outer membrane. Native VirB2, VirB5, VirB7, and VirB8 were also localized in theA. tumefaciensoctopine strain A348. Quantitative analyses of the localization of all the above Vir proteins in nopaline and octopine strains revealed multiple foci in single optical sections in over 80% and 70% of the bacterial cells, respectively. Green fluorescent protein (GFP)-VirB8 expression followingvirinduction was used to monitor bacterial binding to live host plant cells; bacteria bind predominantly along their lengths, with few bacteria binding via their poles or subpoles.vir-induced attachment-defective bacteria or bacteria without the Ti plasmid do not bind to plant cells. These data support a model where multiplevir-T4SS around the perimeter of the bacterium maximize effective contact with the host to facilitate efficient transfer of DNA and protein substrates.IMPORTANCETransfer of DNA and/or proteins to host cells through multiprotein type IV secretion system (T4SS) complexes that span the bacterial cell envelope is critical to bacterial pathogenesis. Early reports suggested that T4SS components localized at the cell poles. Now, higher-resolution deconvolution fluorescence microscopy reveals that all structural components of theAgrobacterium tumefaciens vir-T4SS, as well as its transported protein substrates, localize to multiple foci around the cell perimeter. These results lead to a new model ofA. tumefaciensattachment to a plant cell, whereA. tumefacienstakes advantage of the multiplevir-T4SS along its length to make intimate lateral contact with plant cells and thereby effectively transfer DNA and/or proteins through thevir-T4SS. The T4SS ofA. tumefaciensis among the best-studied T4SS, and the majority of its components are highly conserved in different pathogenic bacterial species. Thus, the results presented can be applied to a broad range of pathogens that utilize T4SS.

scholarly journals Fast-TrACC: A Rapid Method for Delivering and Testing Gene Editing Reagents in Somatic Plant Cells

2021 ◽  
Vol 2 ◽  
Author(s):  
Ryan A. Nasti ◽  
Matthew H. Zinselmeier ◽  
Macy Vollbrecht ◽  
Michael F. Maher ◽  
Daniel F. Voytas
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Rapid Method ◽  
High Throughput ◽  
Promoter Activity ◽  
Gene Editing ◽  
Plant Cells ◽  
Culture Method ◽  
Luciferase Reporter ◽  
Considerable Time ◽  
Or Gene

The production of transgenic or gene edited plants requires considerable time and effort. It is of value to know at the onset of a project whether the transgenes or gene editing reagents are functioning as predicted. To test molecular reagents transiently, we implemented an improved, Agrobacterium tumefaciens-based co-culture method called Fast-TrACC (Fast Treated Agrobacterium Co-Culture). Fast-TrACC delivers reagents to seedlings, allowing high throughput, and uses a luciferase reporter to monitor and calibrate the efficiency of reagent delivery. We demonstrate the use of Fast-TrACC in multiple solanaceous species and apply the method to test promoter activity and the effectiveness of gene editing reagents.

Light strongly promotes gene transfer from Agrobacterium tumefaciens to plant cells

Planta ◽  
2003 ◽  
Vol 216 (4) ◽  
pp. 580-586 ◽  
Author(s):  
Mukund Zambre ◽  
Nancy Terryn ◽  
Janniek De Clercq ◽  
Sylvie De Buck ◽  
Willy Dillen ◽  
...  
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Gene Transfer ◽  
Plant Cells

Erratum: Involvement of circular intermediates in the transfer of T-DNA from Agrobacterium tumefaciens to plant cells

Nature ◽  
1985 ◽  
Vol 313 (6004) ◽  
pp. 714-714
Author(s):  
Zdena Koukolíková-Nicola ◽  
Raymond D. Shillito ◽  
Barbara Hohn ◽  
Kan Wang ◽  
Marc Van Montagu ◽  
...  
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Plant Cells
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