The Characteristics of Succinylated Con A Induced Growth Inhibition of 3T3 Cells in Tissue Culture

1975 ◽  
pp. 207-220 ◽  
Author(s):  
Raphael J. Mannino ◽  
Max M. Burger
Keyword(s):  
Tissue Culture ◽  
Growth Inhibition ◽  
3T3 Cells ◽  
Con A

Lectin Binding Studies on RNA Tumor Virus-Infected Cells

1975 ◽  
Vol 33 ◽  
pp. 326-327
Author(s):  
D. C. Hixson
Keyword(s):  
Binding Sites ◽  
Lectin Binding ◽  
Culture Conditions ◽  
3T3 Cells ◽  
Binding Studies ◽  
Con A ◽  
Microscopic Studies ◽  
Tumor Virus ◽  
Infected Cells ◽  
Cell Culture Conditions

The abilities of plant lectins to preferentially agglutinate malignant cells and to bind to specific monosaccharide or oligosaccharide sequences of glycoproteins and glycolipids make them a new and important biochemical probe for investigating alterations in plasma membrane structure which may result from malignant transformation. Electron and light microscopic studies have demonstrated clustered binding sites on surfaces of SV40-infected or tryp- sinized 3T3 cells when labeled with concanavalin A (con A). No clustering of con A binding sites was observed in normal 3T3 cells. It has been proposed that topological rearrangement of lectin binding sites into clusters enables con A to agglutinate SV40-infected or trypsinized 3T3 cells (1). However, observations by other investigators have not been consistent with this proposal (2) perhaps due to differences in reagents used, cell culture conditions, or labeling techniques. The present work was undertaken to study the lectin binding properties of normal and RNA tumor virus-infected cells and their associated viruses using lectins and ferritin-conjugated lectins of five different specificities.

Differences in density of Concanavalin A-binding sites due to differences in surface morphology of suspended normal and transformed 3T3 fibroblasts

1975 ◽  
Vol 19 (1) ◽  
pp. 21-32
Author(s):  
J.G. Collard ◽  
J.H. Temmink
Keyword(s):  
Surface Morphology ◽  
Concanavalin A ◽  
Binding Sites ◽  
3T3 Cells ◽  
Con A ◽  
3T3 Fibroblasts ◽  
Transformed Fibroblasts ◽  
Transmission Electron ◽  
The Difference ◽  
Electron Microscopes

Calculations of the density of Concanavalin A (Con A)-binding sites on normal and transformed fibroblasts have, as yet, been based on the unproven assumption that suspended cells are smooth spheres. We studied the surface morphology of suspended normal and transformed fibroblasts with scanning and transmission electron microscopes, and found a large difference in surface morphology between suspended normal and transformed 3T3 cells. When this difference in surface morphology was taken into account, the estimated cell surface area of normal 3T3 cells was approximately seven times larger than that of transformed 3T3 cells. Since equal numbers of 3H-Con A molecules are bound on normal and transformed cells, the density of Con A-binding sites is approximately seven times greater on transformed than on normal 3T3 cells. The difference in density of Con A-binding sites between normal and transformed fibroblasts might be sufficient to explain the difference in agglutination response, as originally suggested by Burger, and may also be the cause of the different degrees of clustering of Con A-binding sites on the plasma membrane of these cells.

Effects of Ca++ and Light Exposure of 3T3 Cells in Tissue Culture

2015 ◽  
Vol 21 (S5) ◽  
pp. 75-76 ◽  
Author(s):  
Maria José M.S. Morsoleto ◽  
M.I. Mathias ◽  
F.M.R. Morgado ◽  
A.P. Alves de Matos
Keyword(s):  
Tissue Culture ◽  
Light Exposure ◽  
3T3 Cells

scholarly journals Surface morphology and agglutinability with concanavalin A in normal and transformed murine fibroblasts.

1976 ◽  
Vol 68 (1) ◽  
pp. 101-112 ◽  
Author(s):  
J G Collard ◽  
J H Temmink
Keyword(s):  
Surface Morphology ◽  
Concanavalin A ◽  
Ethylenediaminetetraacetic Acid ◽  
Simian Virus 40 ◽  
Cell Surfaces ◽  
3T3 Cells ◽  
Con A ◽  
Transformed Fibroblasts ◽  
Murine Fibroblasts

The surface morphology of attached and suspended normal and transformed fibroblasts has been studied with the scanning electron microscope. Normal murine fibroblasts (3T3) grow in vitro with widely extended leading lamellae. During most parts of the cell cycle the surfaces of these cells are practically free of microvilli. When the cells round up for mitosis, their cell surfaces become adorned with many microvilli. In contrast, simian virus 40-transformed fibroblasts (SV3T3) grow more compact, and their cell surfaces remain smooth throughout the life cycle. When confluent 3T3 and SV3T3 cells are suspended with ethylenediaminetetraacetic acid (EDTA) for agglutination assays, similar differences in surface morphology are found: 3T3 cells always bear many microvilli, whereas most SV3T3 cells are essentially free of microvilli. The addition of concanavalin A (Con A) does not influence the surface morphology of the suspended cells. The morphological differences described here may be important for the agglutination process of the normal and transformed 3T3 cells, because they affect the real cell surface area and thus the density of Con A-binding sites.

scholarly journals Sorting out of normal and virus-transformed cells in cellular aggregates.

1976 ◽  
Vol 68 (2) ◽  
pp. 276-286 ◽  
Author(s):  
H Gershman ◽  
J Drumm ◽  
L Culp
Keyword(s):  
B Cells ◽  
Cultured Cells ◽  
Cell Types ◽  
Transformed Cells ◽  
3T3 Cells ◽  
Con A ◽  
Adhesive Properties ◽  
Established Cell ◽  
Swiss 3T3 ◽  
Cellular Aggregates

The sorting-out behavior (self-segregation of two cell types from mixtures of the two) of five different established cell lines was studied. Eight of the ten possible binary combinations of these lines, cultured as cellular aggregates, were examined. Mouse BALB/c 3T3 cells sorted out internally to the corresponding malignant SV40 virus-transformed 3T3 cells. The transformed 3T3 line (SVT-2) did not sort out from a revertant line selected from SVT-2 cells by resistance to concanavalin A (con A). The revertant cells sorted out externally to the parent BALB/c 3T3 cells, although segregation was generally incomplete. BALB/c 3T3 cells did not sort out from another contact-inhibited line of 3T3 cells derived from Swiss albino mice (Swiss 3T3). Both BALB/c 3T3 and Swiss 3T3 cells sorted out from cells of the contact-inhibited hamster line, NIL B. Instead of a two-layered sphere, however, a three-layered structure was observed with most of the NIL B cells external to the 3T3 cells, and a few NIL B cells comprising the center of the sphere. On the other hand, NIL B cells did not consistently sort out from either the SVT-2 or con A cells. In general, sorting out between pairs of these five lines are slower and less complete than is generally observed between the more extensively studied chick embryonic tissue cells, suggesting that the cultured cells may be more closely related in their adhesive properties. The internal segregation of BALB/c 3T3 cells relative to SVT-2 cells is consistent with the hypothesis that transformed cells are less adhesive than their nontransformed counterparts.

scholarly journals Penyimpanan In Vitro Tanaman Obat Daun Dewa melalui Pertumbuhan Minimal

2016 ◽  
Vol 1 (2) ◽  
pp. 68
Author(s):  
Endang G. Lestari ◽  
Ragapadmi Purnamaningsih
Keyword(s):  
Tissue Culture ◽  
Growth Inhibition ◽  
Shoot Growth ◽  
Basic Medium ◽  
Ms Medium ◽  
Medicinal Crop ◽  
Plant Shoots ◽  
Optimum Capacity

<p class="p1">Daun dewa (<em>Gynura Procumbens</em>) is a medicinal crop commonly used to remedy cancer, diabetes, and dermatitis. It has a bright prospect for future used. Plant preservations through tissue culture is done to anticipate an urgent need. An experiment was carried out to <em>in vitro </em>preservation of Daun Dewa by the minimum growth and regeneration to examine viability of the culture after the preservation. Terminal shoots (±1 cm) were cultured on a 1/2 MS basic medium + paclobutrazol (0, 1, 2, 3, and 4 mg/l) or ABA (1, 2, and 5 mg/l). The trial was arranged in a, completely randomized with 10 replications. The results showed a three-month preservation of the culture on a medium containing ABA inhibited proliferation and expansion of the plant shoots. Increasing ABA concentrations up to 5 mg/l, according to the shoot-growth inhibition, resulted in the height of 0.6 cm. After three month preservation, the shoots were able to produce roots. After 12 month preservation, the optimum capacity of growth inhibition was shown on 1/2 MS medium + ABA (1, 3, and 5 mg/l). The application of paclobutrazol (1, 2, 3, and 4 mg/l) in the medium produced low multiplication level of shoots, the length of the shoots remains higher than those on 1/2 MS medium without paclobutrazol. Seven months after preservation, viability of the plants was still high when cultured on MS medium + 2 mg/l BA combined with paclobutrazol and ABA as previously given. In addition, the rooted culture could be directly acclimatized in the glasshouse. The lowest number of shoot and shortest shoot after 12 month preservation period was found on the medium containing 5 mg/l ABA and 4 mg/l paclobutrazol, this treatment produced two shoots of 4 cm long. The best medium for the explant regeneration after 7 month preservation was MS + 2 mg/l BA. The plant shoots produced roots directly after they were acclimatized in the glasshouse.</p>

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