Analysis of Bacterial Surface Interactions with Mass Spectrometry-Based Proteomics

The diverse surface interactions and functions of a bacterium play an important role in cell signaling, host infection, and colony formation. To understand and synthetically control biological functions of individual...

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Species-dependent hydrodynamics of flagellum-tethered bacteria in early biofilm development

Journal of The Royal Society Interface ◽

10.1098/rsif.2015.0966 ◽

2016 ◽

Vol 13 (115) ◽

pp. 20150966 ◽

Cited By ~ 15

Author(s):

Rachel R. Bennett ◽

Calvin K. Lee ◽

Jaime De Anda ◽

Kenneth H. Nealson ◽

Fitnat H. Yildiz ◽

...

Keyword(s):

Shewanella Oneidensis ◽

Surface Interactions ◽

Bacterial Surface ◽

Flagellar Filament ◽

Transition Event ◽

Surface Detachment ◽

Species Specific ◽

Biofilm Development ◽

The Impact ◽

Spinning Behaviour

Monotrichous bacteria on surfaces exhibit complex spinning movements. Such spinning motility is often a part of the surface detachment launch sequence of these cells. To understand the impact of spinning motility on bacterial surface interactions, we develop a hydrodynamic model of a surface-bound bacterium, which reproduces behaviours that we observe in Pseudomonas aeruginosa , Shewanella oneidensis and Vibrio cholerae , and provides a detailed dictionary for connecting observed spinning behaviour to bacteria–surface interactions. Our findings indicate that the fraction of the flagellar filament adhered to the surface, the rotation torque of this appendage, the flexibility of the flagellar hook and the shape of the bacterial cell dictate the likelihood that a microbe will detach and the optimum orientation that it should have during detachment. These findings are important for understanding species-specific reversible attachment, the key transition event between the planktonic and biofilm lifestyle for motile, rod-shaped organisms.

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Effect of Argon and Hydrogen on Deposition of Silicon from Tetrachlorosilane in Cold Plasmas

MRS Proceedings ◽

10.1557/proc-54-715 ◽

1985 ◽

Vol 54 ◽

Cited By ~ 1

Author(s):

R. R. Manory ◽

R. Avni ◽

A. Grill

Keyword(s):

Mass Spectrometry ◽

Cold Plasma ◽

Surface Interactions ◽

Plasma Surface ◽

Plasma Surface Interactions ◽

Cold Plasmas

ABSTRACTThe roles of Ar and H2 on the decomposition of SiCl4 1n cold plasma were Investigated by Langmulr probes and mass spectrometry. Decomposition of the reactant by Ar only has been found to be very slow. In presence of H2 in the plasma SiCl4 is decomposed by fast radical-molecule reactions which are further enhanced by Ar due to additional 1on-molecule reactions in which more H radicals are produced. A model for the plasma-surface Interactions during deposition of pC-S1 1n the Ar + H2 + SiCl4 system is presented.

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How SIMS microscopy can be used in medicine

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100132649 ◽

1992 ◽

Vol 50 (2) ◽

pp. 1602-1603

Author(s):

Philippe Fragu

Keyword(s):

Mass Spectrometry ◽

Biomedical Applications ◽

Secondary Ion Mass Spectrometry ◽

Chemical Elements ◽

Biological Applications ◽

The Past ◽

Potential Source ◽

Secondary Ion ◽

Chemical Integrity ◽

Scientific Endeavour

The identification, localization and quantification of intracellular chemical elements is an area of scientific endeavour which has not ceased to develop over the past 30 years. Secondary Ion Mass Spectrometry (SIMS) microscopy is widely used for elemental localization problems in geochemistry, metallurgy and electronics. Although the first commercial instruments were available in 1968, biological applications have been gradual as investigators have systematically examined the potential source of artefacts inherent in the method and sought to develop strategies for the analysis of soft biological material with a lateral resolution equivalent to that of the light microscope. In 1992, the prospects offered by this technique are even more encouraging as prototypes of new ion probes appear capable of achieving the ultimate goal, namely the quantitative analysis of micron and submicron regions. The purpose of this review is to underline the requirements for biomedical applications of SIMS microscopy.Sample preparation methodology should preserve both the structural and the chemical integrity of the tissue.

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