Fluorescence Correlation and Cross-Correlation Spectroscopy in Zebrafish

2018 ◽  
pp. 67-105
Author(s):  
Xue Wen Ng ◽  
Karuna Sampath ◽  
Thorsten Wohland
Keyword(s):  
Cross Correlation ◽  
Correlation Spectroscopy ◽  
Fluorescence Correlation

scholarly journals Fluorescence Correlation and Cross-Correlation Spectroscopy Unveil Cytoplasmic mRNP Composition and Dynamics

2020 ◽  
Author(s):  
Àngels Mateu-Regué ◽  
Jan Christiansen ◽  
Christian Hellriegel ◽  
Finn Cilius Nielsen
Keyword(s):  
Life Cycle ◽  
Dynamic Analysis ◽  
Cross Correlation ◽  
Rna Binding ◽  
Rna Binding Proteins ◽  
Correlation Spectroscopy ◽  
Live Cells ◽  
Macromolecular Complexes ◽  
Fluorescence Correlation ◽  
Macromolecular Composition

ABSTRACTUnderstanding the mRNA life cycle requires analysis of the dynamic macromolecular composition and stoichiometry of mRNPs. Fluorescence correlation and cross-correlation spectroscopy (FCS and FCCS) are appealing technologies to study mRNP complexes because they readily provide information about the molecular composition, stoichiometry, heterogeneity and dynamics of the particles. We developed FCS protocols for analysis of live cells and cellular lysates, and demonstrate the feasibility of analysing common cytoplasmic mRNPs composed of core factor YBX1, IMPs (or IGF2BPs) and their interactions with other RNA binding proteins such as PABPC1, ELAVL2 (HuB), STAU1 and FMRP. FCCS corroborated previously reported RNA dependent interactions between the factors and provided an estimate of the relative overlap between the factors in the mRNPs. In this way FCS and FCCS provide a new and useful approach for the quantitative and dynamic analysis of mRNP macromolecular complexes that may complement current biochemical approaches.

scholarly journals Interferometric Fluorescent Cross Correlation Spectroscopy

2019 ◽  
Author(s):  
Ipsita Saha ◽  
Saveez Saffarian
Keyword(s):  
Quantum Dots ◽  
Fluorescence Correlation Spectroscopy ◽  
Cross Correlation ◽  
Single Photon ◽  
Theoretical Framework ◽  
Correlation Spectroscopy ◽  
Aqueous Samples ◽  
Cellular Membranes ◽  
Fluorescence Correlation ◽  
Fluorescent Molecules

AbstractWe present a method utilizing single photon interference and fluorescence correlation spectroscopy (FCS) to simultaneously measure transport of fluorescent molecules within aqueous samples. Our method, within seconds, measures transport in thousands of homogenous voxels (100 nm)3 and eliminates photo-physical artifacts associated with blinking of fluorescent molecules. A comprehensive theoretical framework is presented and validated by measuring transport of quantum dots, associated with VSV-G receptor along cellular membranes as well as within viscous gels.

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