Phenotypic Characteristics of Neonatal T Cells

2007 ◽  
pp. 193-218
Keyword(s):  
T Cells ◽  
Phenotypic Characteristics

scholarly journals CD4+ T cell immunity to Salmonella is transient in the circulation

2021 ◽  
Vol 17 (10) ◽  
pp. e1010004
Author(s):  
Newton G. Peres ◽  
Nancy Wang ◽  
Paul Whitney ◽  
Sven Engel ◽  
Meghanashree M. Shreenivas ◽  
...  
Keyword(s):  
T Cells ◽  
Bacterial Pathogen ◽  
Cd4 T Cell ◽  
Phenotypic Characteristics ◽  
Peripheral Tissues ◽  
Cell Immunity ◽  
Serovar Typhimurium ◽  
Ifn Γ ◽  
Systemic Infections ◽  
Over Time

While Salmonella enterica is seen as an archetypal facultative intracellular bacterial pathogen where protection is mediated by CD4+ T cells, identifying circulating protective cells has proved very difficult, inhibiting steps to identify key antigen specificities. Exploiting a mouse model of vaccination, we show that the spleens of C57BL/6 mice vaccinated with live-attenuated Salmonella serovar Typhimurium (S. Typhimurium) strains carried a pool of IFN-γ+ CD4+ T cells that could adoptively transfer protection, but only transiently. Circulating Salmonella-reactive CD4+ T cells expressed the liver-homing chemokine receptor CXCR6, accumulated over time in the liver and assumed phenotypic characteristics associated with tissue-associated T cells. Liver memory CD4+ T cells showed TCR selection bias and their accumulation in the liver could be inhibited by blocking CXCL16. These data showed that the circulation of CD4+ T cells mediating immunity to Salmonella is limited to a brief window after which Salmonella-specific CD4+ T cells migrate to peripheral tissues. Our observations highlight the importance of triggering tissue-specific immunity against systemic infections.

scholarly journals An idiotype-specific helper population that bears immunoglobulin, Ia, and Lyt-1 determinants.

1984 ◽  
Vol 159 (4) ◽  
pp. 1189-1200 ◽  
Author(s):  
D H Sherr ◽  
M E Dorf
Keyword(s):  
T Cells ◽  
B Cells ◽  
Cell Population ◽  
T Helper Cells ◽  
T Helper ◽  
Phenotypic Characteristics ◽  
Surface Phenotype ◽  
Specific Recognition ◽  
Helper Cells ◽  
Helper Activity

A helper cell population with phenotypic characteristics of both B and T cells is described. This helper population, called BH, is present in normal unprimed C57BL/6 mice and preferentially helps the expression of NPb idiotype-bearing plaque-forming B cells in the absence of T helper cells. Its surface phenotype is Lyt-1.2+, Ig+, Lyb-3+, Thy-1.2-, Lyt-2.2-. The helper activity of the BH population is IgH restricted and BH cells selectively bind NPb idiotypic determinants. Collectively the data demonstrate that this unique subpopulation can regulate the response of antibody-secreting B cells through specific recognition of idiotypic determinants.

Leukemic blasts fail to differentiate into mature antigen presenting cells, and may hinder dendritic cell maturation in vitro and in vivo

2007 ◽  
Vol 25 (18_suppl) ◽  
pp. 10556-10556
Author(s):  
J. Rosenblatt ◽  
R. Stone ◽  
C. Lenahan ◽  
Z. Wu ◽  
B. Vasir ◽  
...  
Keyword(s):  
T Cells ◽  
Dendritic Cells ◽  
Tumor Vaccine ◽  
Leukemia Cells ◽  
Phenotypic Characteristics ◽  
Gm Csf ◽  
Maturation In Vitro ◽  
Dc Maturation

10556 Background: Dendritic cells (DC) play a key role in the development of tumor specific immune responses. Dendritic cells differentiated from leukemic blasts (LDC) are being explored as a tumor vaccine in AML. We examined the phenotypic and functional characteristics of LDC, the phenotypic characteristics of native DC in AML patients, and the effect of leukemic blasts on the phenotype of DC generated from normal donors. Methods: Leukemia blasts were isolated from peripheral blood of 24 patients with AML. LDC were generated by culturing blasts in the presence of GM-CSF, IL-4 and TNFa for 7 days. The phenotype of circulating DC1 (CD11C+/lin-) and DC2 (CD123+/ lin-) in AML patients was assessed by multichannel FACS analysis. To assess the effect of blasts on DC maturation, adherent mononuclear cells were isolated from normal donors, combined with leukemia cells in a 10:1 ratio, and cultured with GM-CSF, IL-4, and TNFa. Results: LDC demonstrate only modest expression of the costimulatory molecules CD80 and CD86 (mean expression 10% and 32%) and poorly express the maturation marker CD83 (mean expression 4%). Interferon gamma production by autologous T cells was not higher after stimulation with LDC than with blasts. LDC stimlation resulted in a 2 fold increase in both CD4+/CD25+/CD69+ (activated) and CD4+/CD25+/FOXP3+ (regulatory) T cells. Given the inability of leukemia progenitors to differentiate into phenotypically mature DC, we assessed whether leukemia cells directly inhibit differentiation of DC from normal progenitors. Expression of costimulatory molecules was decreased in DC differentiated in the presence of blasts. Mean expression of CD80, CD83, and CD86 was 16%, 2%, 83% and 49%, 10%, 99% for DCs generated in the presence or absence blasts respectively. Phenotypic characteristics of native DC in patients with AML were examined. In 3 experiments, a predominance of DC2 was seen (ratio DC2/DC1 5), and both DC1 and DC2 poorly expressed CD83 (mean expression 9% DC1, 0.9% DC2). Conclusions: LDC have phenotypic and functional deficiencies, limiting their efficacy as a tumor vaccine. Contact with leukemic blasts may inhibit DC maturation in vitro and in vivo, which may contribute to the lack of effective antitumor immunity in AML patients. No significant financial relationships to disclose.

Mel14+CD4+ T cells from aged mice display functional and phenotypic characteristics of memory cells

1994 ◽  
Vol 6 (8) ◽  
pp. 1227-1234 ◽  
Author(s):  
Ruud Dobber ◽  
Margret Tielemans ◽  
Hellen de Weerd ◽  
Lex Nagelkerken
Keyword(s):  
T Cells ◽  
Cd4 T Cells ◽  
Memory Cells ◽  
Phenotypic Characteristics ◽  
Aged Mice

Virus-Specific CD4+ T Cells Have Functional and Phenotypic Characteristics of Follicular T-Helper Cells in Patients With Acute and Chronic HCV Infections

2016 ◽  
Vol 150 (3) ◽  
pp. 696-706.e3 ◽  
Author(s):  
Bijan Raziorrouh ◽  
Kathrin Sacher ◽  
Rajiv G. Tawar ◽  
Florian Emmerich ◽  
Christoph Neumann-Haefelin ◽  
...  
Keyword(s):  
T Cells ◽  
T Helper Cells ◽  
Cd4 T Cells ◽  
T Helper ◽  
Phenotypic Characteristics ◽  
Helper Cells ◽  
Chronic Hcv ◽  
Follicular T Helper Cells

scholarly journals Regulat ory T-cells in periphera l blood of pat ientswith br onchial ast hma

2011 ◽  
Vol 8 (5) ◽  
pp. 21-25
Author(s):  
E E Kremer ◽  
N A Kirillova ◽  
N V Teplova ◽  
I A Deev ◽  
I V Petrova ◽  
...  
Keyword(s):  
T Cells ◽  
Peripheral Blood ◽  
Allergic Inflammation ◽  
Scientific Data ◽  
Control Mechanisms ◽  
Phenotypic Characteristics ◽  
Becton Dickinson ◽  
Surface Molecules ◽  
Healthy Donors ◽  
Healthy Control

Background. To investigate the expression of surface molecules CD4+CD25high and CD4+FoxP3+ on regulatory T-cells (Treg) in patients with different severity of bronchial asthma (BA). Methods. We included 19 patients with severe BA, 12 patients with mild BA and 17 healthy donors. The circulating percentage of CD4+CD25high and CD4+FoxP3+ T-reg in peripheral blood was estimated by the flow cytometry analysis (FACSCalibur, Becton Dickinson, USA). Results. Numbers of peripheral blood CD4+CD25high was significantly decreased in mild BA compared to healthy control. Patients with severe BA had increased levels of CD4+FoxP3+ and CD4+CD25high compared to mild BA. Conclusion. Our results demonstrate new scientific data on the phenotypic characteristics and the level of T-reg in peripheral blood at different clinical forms of asthma. This fact confirms the existence of immunoregulatory control mechanisms of allergic inflammation involving T-reg.

scholarly journals Whole-blood phenotyping to assess alloimmunization status in transfused sickle cell disease patients

2021 ◽  
Vol 5 (5) ◽  
pp. 1278-1282 ◽  
Author(s):  
Marie Tamagne ◽  
Sadaf Pakdaman ◽  
Pablo Bartolucci ◽  
Anoosha Habibi ◽  
Frédéric Galactéros ◽  
...  
Keyword(s):  
T Cells ◽  
T Lymphocyte ◽  
Whole Blood ◽  
T Helper ◽  
Cell Disease ◽  
Phenotypic Characteristics ◽  
Tfh Cells ◽  
Low Levels ◽  
Inhibitory Signaling ◽  
Transfusion Reactions

Abstract It is essential to limit hemolytic transfusion reactions in polytransfused individuals, and the prevention of alloimmunization is a key solution. CD4+ T lymphocyte (TL) markers, particularly follicular T helper (Tfh) cells, may differentiate between responder and nonresponder alloimmunization statuses. We tested this hypothesis by studying the phenotype of CXCR5+PD1+ TLs in whole blood. Our results suggest that high levels of CXCR5+PD1+CD4+ TLs in whole blood may be a characteristic of nonalloimmunized patients. However, these cells did not display the phenotypic characteristics of active Tfh cells. Instead, a decrease in blood quiescent Tfh-cell levels was observed in nonalloimmunized polytransfused patients. High levels of CXCR5+PD1+CD4+ TLs may be associated with inhibitory signaling functions of T cells, as reflected by the low levels of PD1+ICOS+ cells in the nonalloimmunized polytransfused group. The description of these particular phenotypes, and their comparison among groups of patients, responders, and nonresponders, suggests that new immunological components should be considered when trying to understand posttransfusion alloimmunization.

scholarly journals cDC1 Dependent Accumulation of Memory T Cells Is Required for Chronic Autoimmune Inflammation in Murine Testis

2021 ◽  
Vol 12 ◽  
Author(s):  
Yuchao Jing ◽  
Min Cao ◽  
Bei Zhang ◽  
Xuehui Long ◽  
Xiaoming Wang
Keyword(s):  
T Cells ◽  
Memory T Cells ◽  
Physiological State ◽  
Phenotypic Characteristics ◽  
Autoimmune Inflammation ◽  
Comprehensive Picture ◽  
Autoimmune Orchitis ◽  
Primary Type ◽  
And Function ◽  
With Memory

As an immune privilege site, there are various types of immune cells in the testis. Previous research has been focused on the testicular macrophages, and much less is known about the T cells in the testis. Here, we found that T cells with memory phenotypes were the most abundant leukocyte in the testis except for macrophages. Our results showed that the proportion of testicular T cells increases gradually from birth to adulthood in mice and that the primary type of T cells changed from γδTCR+ T cells to αβTCR+ T cells. In addition, under homeostatic conditions, CD8+ T cells are the dominant subgroup and have different phenotypic characteristics from CD4+ T cells. We found that cDC1, but not cDC2, is necessary for the presence of T cells in the testis under physiological state. A significant decrease of T cells does not have a deleterious effect on the development of the testis or spermatogenesis. However, cDC1-dependent T cells play an indispensable role in chronic autoimmune orchitis of the testis. Collectively, our multifaceted data provide a comprehensive picture of the accumulation, localization, and function of testicular T cells.

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