Enzymatic Hydrolysis and Ethanol Fermentation of High Dry Matter Wet-Exploded Wheat Straw at Low Enzyme Loading

2007 ◽  
pp. 553-562 ◽  
Author(s):  
Tania I. Georgieva ◽  
Xiaoru Hou ◽  
Troels Hilstrøm ◽  
Birgitte K. Ahring
Keyword(s):  
Enzymatic Hydrolysis ◽  
Wheat Straw ◽  
Ethanol Fermentation ◽  
Dry Matter ◽  
Enzyme Loading ◽  
High Dry Matter

Enzymatic Hydrolysis and Ethanol Fermentation of High Dry Matter Wet-Exploded Wheat Straw at Low Enzyme Loading

2007 ◽  
Vol 148 (1-3) ◽  
pp. 35-44 ◽  
Author(s):  
Tania I. Georgieva ◽  
Xiaoru Hou ◽  
Troels Hilstrøm ◽  
Birgitte K. Ahring
Keyword(s):  
Enzymatic Hydrolysis ◽  
Wheat Straw ◽  
Ethanol Fermentation ◽  
Dry Matter ◽  
Enzyme Loading ◽  
High Dry Matter

scholarly journals Enzymatic hydrolysis at high dry matter content: The influence of the substrates’ physical properties and of loading strategies on mixing and energetic consumption

2018 ◽  
Vol 250 ◽  
pp. 191-196 ◽  
Author(s):  
Federico Battista ◽  
Mélanie Gomez Almendros ◽  
Romain Rousset ◽  
Serge Boivineau ◽  
Pierre-Antoine Bouillon
Keyword(s):  
Enzymatic Hydrolysis ◽  
Physical Properties ◽  
Dry Matter ◽  
Matter Content ◽  
Dry Matter Content ◽  
High Dry Matter

scholarly journals Optimization of Sodium Hydroxide Pretreatment and Enzymatic Hydrolysis of Wheat Straw Powder by Cellulases and Xylanases From Aspergillus Niger HQ-1 Using Response Surface Methodology

2021 ◽  
Author(s):  
Hui Zhang ◽  
Junhui Wu
Keyword(s):  
Response Surface Methodology ◽  
Enzymatic Hydrolysis ◽  
Aspergillus Niger ◽  
Sodium Hydroxide ◽  
Wheat Straw ◽  
Response Surface ◽  
Sodium Hydroxide Solution ◽  
Solid Loading ◽  
Enzyme Loading ◽  
Hydrolysis Of

Abstract To maximize fermentable sugars production, response surface methodology (RSM) was adopted to optimize pretreatment and enzymatic hydrolysis of wheat straw powder (WSP) using the crude cellulases preparation containing xylanases from Aspergillus niger HQ-1. Factors of pretreatment including sodium hydroxide concentration, pretreatment time and temperature were found to have significant effects on sugars production. Results indicated that WSP with particle size 0.3 mm should be pretreated using 1.8% (w/v) sodium hydroxide solution with 25.0% (w/v) of solid loading at 94.0°C for 46.0 min and the optimized pretreatment conditions could result in 90.9% of cellulose recovery, 54.6% of hemicellulose recovery and 72.7% of lignin removal, respectively. Furthermore, variables of enzymatic hydrolysis including enzyme loading, biomass loading and reaction time were proved to have significant effects on sugars yields. After hydrolysis at 50°C for 44.8 h with 7.1% (w/v) of biomass loading, 8.1 FPU/g of enzyme loading and 0.2% (w/v) of Tween-80, maximum yields of reducing sugar (632.92 mg/g) and xylose (149.83 mg/g) could be obtained, respectively. In addition, holocellulose and hemicellulose conversion were 81.6% and 80.0%, respectively. To the best of our knowledge, this is the first report about systematic optimization of sodium hydroxide pretreatment and enzymatic hydrolysis of WSP using RSM.

In situ laccase treatment enhances the fermentability of steam-exploded wheat straw in SSCF processes at high dry matter consistencies

2013 ◽  
Vol 143 ◽  
pp. 337-343 ◽  
Author(s):  
Antonio D. Moreno ◽  
Elia Tomás-Pejó ◽  
David Ibarra ◽  
Mercedes Ballesteros ◽  
Lisbeth Olsson
Keyword(s):  
Wheat Straw ◽  
Dry Matter ◽  
High Dry Matter

scholarly journals Optimisation of enzymatic saccharification of wheat straw pre-treated with sodium hydroxide

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Zhiquan Wang ◽  
Suqing Wu ◽  
Chunzhen Fan ◽  
Xiangyong Zheng ◽  
Wei Zhang ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Wheat Straw ◽  
Treatment Time ◽  
Solid Content ◽  
Reducing Sugar ◽  
Sugar Yield ◽  
Experimental Result ◽  
Enzyme Loading ◽  
Hydrolysis Time ◽  
Pre Treatment

AbstractTo enhance the reducing sugar yield in enzymatic hydrolysis, various factors (NaOH concentration, solid content and pre-treatment time) that affect the pre-treatment process were investigated and evaluated based on the reducing sugar yield of the subsequent enzymatic hydrolysis. The enzymatic hydrolysis was based on the cellulase from Trichoderma reesi ATCC 26921, the optimum NaOH pre-treatment conditions were an NaOH concentration of 1.0% (w/w), a solid content of 5.0% (w/v) and a pre-treatment time of 60 min. Various parameters that affect the enzymatic hydrolysis of wheat straw, including the solid content, enzyme loading, pH and hydrolysis time, were investigated and optimized through a Box–Behnken design and response surface methodology. The predicted optimum conditions for enzymatic hydrolysis were a solid content of 8.0% (w/v), an enzyme loading of 35 FPU/g substrate, a temperature of 50 °C, a pH of 5.3 and a hydrolysis time of 96 h. The experimental result showed that the maximum reducing sugar yield was 60.73% (53.35% higher than the wheat straw without NaOH pre-treatment), which is in accordance with the predicted conditions.

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